Fig 1: Western blot analysis of mitochondrial proteins. (A) Twenty µg of total proteins from mutant and wild type zebrafish were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with antibodies for 6 subunits of OXPHOS (four encoded by mtDNA and two encoded by nuclear genes), Kars, Lars2, Yars2, Eftu, Tfb2m as well as Tom20 as a loading control. Quantification of levels of OXPHOS subunits (B) and other mitochondrial proteins (C). Average content of Nd1, Nd6, Cytb, Co2 and Atp5a, Sdhb, Kars, Lars2, Yars2, Eftu and Tfb2m was normalized to the average content of Tom20 in mutant and wild type zebrafish. The values for the mutant zebrafish are expressed as percentages of the average values for the wild type zebrafish. The horizontal dashed lines represent the average values of four proteins encoded by mtDNA or two proteins encoded by nuclear genes for each group. The calculations were based on three independent determinations. Graph details and symbols are explained in the legend to the Figure 3.