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Antibodies
Anti-PAI1 antibody
Anti-PAI1 antibody from Abcam
Be the first to write a review!
Citations:
(66)
Supplier Page
Supplier Page from
Abcam for
Anti-PAI1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-PAI1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab66705
Antibody Type
Primary
Host
Rabbit
Quantity
100 UG
Isotype
IgG
Conjugate/Tag
Unconjugated
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Concentration
1 mg/ml
Gene Name
SERPINE1
Reactivity
Human
Target
PAI1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (66)
(1)
Disruption of the Clock Component Bmal1 in Mice Promotes Cancer Metastasis through the PAI-1-TGF-β-myoCAF-Dependent Mechanism
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
August 1, 2023
Wu J, Jing X, Du Q, Sun X, Holgersson K, Gao J, He X, Hosaka K, Zhao C, Tao W,
et al
.
620 184, BIO‐RAD).
Immunoblotting
was carried out with primary antibodies specific for
PAI‐1
(Cat.
Ab66705
,
Abcam
), α‐SMA (Cat.
MA5‐11547
, Thermo Fisher Scientific), FAP (Cat.
NBP2‐89135
,
Novus Biologicals
),
(2)
An Imbalance in the Pro/mature BDNF Ratio Occurs in Multiple Brain Regions During Normal Ageing in Wild-Type Mice
Journal of Molecular Neuroscience
June 14, 2023
Shaun Cade, Xin-Fu Zhou, Larisa Bobrovskaya
azide) for 1 h at room temperature, shaking.
Membranes
were incubated with Rabbit anti-PAI-1 (
Abcam
, cat#
ab66705
,
1:1,500
), Rabbit anti-p75NTR (Home-made,
1:1,000
), Rabbit
anti-Sortilin
(Home-made,
1:10,000
), or Goat
(3)
SIRT6-PAI-1 axis is a promising therapeutic target in aging-related bone metabolic disruption
Scientific reports
May 17, 2023
Aobulikasimu A, Liu T, Piao J, Sato S, Ochi H, Okawa A, Tsuji K, Asou Y.
(
R&D systems
; catalog Number:
AF1589
) (
1:200
), and anti-PAI-1 (
Abcam
Biochemicals; catalog number:
ab66705
) (
1:200
). After washing with T-PBS buffer for three times (5 min each), the sections were incubated
(4)
Influenza A(H1N1)pdm09 Virus Aggravates Pathology of Blood Vessels in Wistar Rats with Premorbid Acute Cardiomyopathy
Viruses
May 4, 2023
Vladimir Marchenko, Irina Zelinskaya, Yana Toropova, Ekaterina Podyacheva, Mikhail Martynov, Daria Mukhametdinova, Dmitry Lioznov, Irina N. Zhilinskaya
antibody (
Abcam
, Bostin, MA, USA,
ab76198
), polyclonal rabbit anti-PAI-1 antibody (
Abcam
, Bostin, MA, USA,
ab66705
), and monoclonal mouse anti-tPA antibody (Novus Biologicals, Littleton, CO, USA, AH54-10) were used.
(5)
SARS‐CoV‐2 infection results in upregulation of Plasminogen Activator Inhibitor‐1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated with disease severity
eJHaem
May 1, 2023
Kevin H. Toomer, Gloria F. Gerber, Yifan Zhang, Laetitia Daou, Michael Tushek, Jody E. Hooper, Ivo M. B. Francischetti
Diagnostics) at 96°C for 64 min. Primary antibodies, including rabbit polyclonal anti‐PAI‐1 (
1:200
,
ab66705
;
Abcam
), rabbit polyclonal anti‐Neuroserpin (
1:250
, PA‐5110636; ThermoFisher), and rabbit polyclonal
(6)
Nasal symbiont Staphylococcus epidermidis restricts the cellular entry of influenza virus into the nasal epithelium
npj Biofilms and Microbiomes
December 1, 2022
Ara Jo, Jina Won, Chan Hee Gil, Su Keun Kim, Kang-Mu Lee, Sang Sun Yoon, Hyun Jik Kim
(molecular weight 45 kDa, primary antibody
1:500
) was purchased from
Abcam
(Waltham, MA, USA, cat #
ab66705
) and
mouse
Serpine1 blocking peptide from
MyBioSource
(San Diego, CA, USA, cat #MBS8309331). San Diego,
(7)
Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats
Viruses
November 14, 2022
Vladimir Marchenko, Darya Mukhametdinova, Irina Amosova, Dmitry Lioznov, Irina Zhilinskaya
anti-eNOS antibody (
Abcam
, Bostin, MA, USA,
ab76198
), polyclonal rabbit anti-PAI-1 antibody (
Abcam
, USA,
ab66705
) and monoclonal mouse anti-tPA antibody (Novus Biologicals, Littleton, CO, USA, AH54-10) were used.
(8)
Connexin 43 overexpression induces lung cancer angiogenesis in vitro following phosphorylation at Ser279 in its C-terminus
Oncology letters
September 1, 2022
Zhou Z, Chai W, Liu Y, Liu Y, Pan H, Wu Q, Zhang X.
Inc.), vWF antibody (product no.
65707
;
Cell Signaling Technology
, Inc.), PAI-1 antibody (product code
ab66705
;
Abcam
), phosphorylated (p)-
Cx43
(Tyr265) antibody (cat. no.
AF2306
;
Affinity Biosciences
), p-
Cx43
(Ser279)
(9)
Secretory SERPINE1 Expression Is Increased by Antiplatelet Therapy, Inducing MMP1 Expression and Increasing Colon Cancer Metastasis
International journal of molecular sciences
August 24, 2022
Kim WT, Mun JY, Baek SW, Kim MH, Yang GE, Jeong MS, Choi SY, Han JY, Kim MH, Leem SH.
Waltham, MA, USA). Primary antibodies against
GAPDH
(Cat #AbC-1001, AbClon, Seoul, Korea), SERPINE1 (Cat #
Ab66705
,
Abcam
, Cambridge, MA, USA),
MMP1
(Cat #
sc137044
,
Santa Cruz Biotechnology
, Santa Cruz, CA, USA),
p38
(10)
The PLAUR signaling promotes chronic pruritus
The FASEB Journal
June 1, 2022
Weiwei Chen et al.
followed by
incubation
with rabbit antibody to
TLR2
(
1:300
,
Abcam
ab213676
), Serpin E1 (
1:300
,
Abcam
ab66705
),
PLAUR
(
1:300
, Abcam
ab103791
),
BNP
(
1:100
,
Abcam
ab236101
),
OSM
(
1:75
,
Thermo
PA576861
),
mouse
antibody
(11)
Upregulation of Atypical Cadherin FAT1 Promotes an Immunosuppressive Tumor Microenvironment via TGF-β
Frontiers in Immunology
May 26, 2022
Khushboo Irshad et al.
TGF-β2 (
mouse
monoclonal,
1:1,000
,
Abcam
, #
ab36495
), Serpine1/PAI-1 (rabbit polyclonal,
1:1,000
,
Abcam
, #
ab66705
), SMAD2/3 (rabbit monoclonal,
1:1,000
,
Cell Signaling Technology
, #
8685
), and β-actin (
mouse
monoclonal,
(12)
The uPA System Differentially Alters Fibroblast Fate and Profibrotic Ability in Skin Fibrosis
Frontiers in Immunology
March 16, 2022
Ming-Li Zou et al.
were as follows: anti-uPA (
PA5-34638
,
Invitrogen
),
anti-uPAR
(
ab103791
,
ab221680
,
Abcam
), anti-PAI-1 (
ab66705
,
Abcam
), anti-Collagen I (
ab260043
,
Abcam
; bs-10423, Bioss), anti-α-SMA (
ab124964
,
Abcam
;
19245
,
Cell Signaling
(13)
Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma
Cancers (Basel)
January 17, 2022
Marine Lottin et al.
Sigma Aldrich), anti-uPA (
Ab169754
,
Abcam
, Paris, France), anti-
tPA
(
Ab157469
,
Abcam
), anti-PAI-1 (
Ab66705
,
Abcam
), anti-PAR-1 (E9J9L Rabbit mAb, #
79109
,
Cell Signaling Technology
, Danvers, MA, USA), anti-PD-L1
(14)
Clostridium butyricum RH2 Alleviates Chronic Foot Shock Stress-Induced Behavioral Deficits in Rats via PAI-1
Frontiers in pharmacology
January 1, 2022
Zhang W, Ding T, Zhang H, Chen Y, Liu L, Jiang J, Song S, Cheng H, Wu C, Sun J,
et al
.
SANTA CRUZ
, USA;
1:200
),
mouse
anti-β-Actin (AF0003, Beyotime, China;
1:1,000
) antibodies, rabbit anti-PAI-1 (
ab66705
,
Abcam
, United Kingdom;
1:1,000
) and rabbit anti-tPA (
10147-1-AP
,
Proteintech
, China,
1:1,000
), respectively.
(15)
Role of intestinal extracellular matrix-related signaling in porcine epidemic diarrhea virus infection
Virulence
December 1, 2021
Li Y, Li J, Wang X, Wu Q, Yang Q.
quantify the expression of
CD44
and PAI-1 proteins, anti-pig
CD44
(cat. no.
ab157107
) and PAI-1 (cat. no.
ab66705
) mAbs were purchased from
Abcam
(Cambridge, UK). The nuclear factor (NF)-κB pathway was assessed using
(16)
Hepsin regulates TGFβ signaling via fibronectin proteolysis
EMBO reports
November 4, 2021
Denis Belitškin et al.
anti‐
SNAIL
(
CST
#
3879S
), anti‐
GAPDH
(
CST
#
2118S
), anti‐β‐tubulin (
Abcam
,
ab6046
), anti‐PAI‐1 (
Abcam
,
ab66705
), and anti‐V5 (
Invitrogen
, #
R96025
).
Animal models
and experiments All work and sample isolation from
(17)
Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer
Glia
September 1, 2021
Connolly NP, Galisteo R, Xu S, Bar EE, Peng S, Tran NL, Ames HM, Kim AJ, Woodworth GF, Winkles JA.
X-100/0.02% Tween and labeled overnight at 4°C with the following primary antibodies: PAI-1 (
1:1000
;
ab66705
,
Abcam
), NF-κB
p100
/52 (
1:100
;
NB100-82063
,
Novus
Biological),
CD163
(
1:100
;
bs-2527R
-TR,
Bioss
),
CD68
(18)
Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production
International Journal of Molecular Sciences
August 31, 2021
Kazuki Kai et al.
1:1000
dilution;
Abcam
), rabbit anti-IL-8 (
ab106350
,
1:500
dilution;
Abcam
), and rabbit anti-PAI-1 (
ab66705
,
1:1000
dilution;
Abcam
). Samples were washed with TBST, and 100–400 μL of DAB (Peroxidase Stain DAB
(19)
p16-3MR: A Novel Model to Study Cellular Senescence in Cigarette Smoke-Induced Lung Injuries
International journal of molecular sciences
May 3, 2021
Kaur G, Sundar IK, Rahman I.
anti-p21 (Cat#
556430
,
BD Biosciences
, San Jose, CA, USA), anti-Fn1 (Cat#
ab2413
), anti-PAI-1 (Cat#
ab66705
), anti-NF-κB(Cat#
ab32360
), anti-MMP9 (Cat#
ab38898
) (
Abcam
, Cambridge, UK), anti-
MMP12
(Cat#
NBP2-67344
,
(20)
Circadian time series proteomics reveals daily dynamics in cartilage physiology
Osteoarthritis and Cartilage
May 1, 2021
M. Dudek, C. Angelucci, D. Pathiranage, P. Wang, V. Mallikarjun, C. Lawless, J. Swift, K.E. Kadler, R.P. Boot-Handford, J.A. Hoyland,
et al.
performed according to standard
procedures
. Primary antibodies for CTGF (
Abcam
ab6992
), PAI-1 (
Abcam
ab66705
),
MATN1
(
Abcam
ab106384
) and alpha tubulin (
Sigma
T9026
) were used in
1:2000
dilution. Secondary antibodies
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Reviews
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Anti-PAI1 antibody from Abcam
Supplier Page
View Company Product Page
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Product Specs
Company Info
Citations
Reviews
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