Fig 1: PACSIN1 is essential for assembly of SNARE complex.(A) WT or PACSIN1 KO HeLa cells were transfected with GFP-STX17. The lysates were immunoprecipitated with GFP-trap beads and immunoblotted with the indicated antibodies. (B) Quantification data show the amount of SNAP29 or VAMP8 normalized by GFP. Mean ± s.e.m (n = 3). *p < 0.05 (two-tailed, unpaired t-test). (C) WT or PACSIN1 KO HeLa cells were transfected with GFP-YKT6. The lysates were immunoprecipitated with GFP-trap beads and immunoblotted with the indicated antibodies. (D) Quantification data show the amount of SNAP29 or STX7 normalized by GFP. Mean ± s.e.m (n = 3). *p < 0.05 (two-tailed, unpaired t-test). (E) WT HeLa cells were transfected with FLAG-SNAP29 and GFP-PACSIN1. The lysates were immunoprecipitated with FLAG-M2 beads and immunoblotted with anti-GFP antibody.