GFP Antibody 600-901-215 from Rockland Immunochemicals, Inc.

Product Specs
ItemGFP Antibody
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number600-901-215
Quantity100 µg
ImmunogenThe immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
HostChicken
Conjugate/TagUnconjugated
ClonalityPolyclonal
ApplicationsELISA,Immunohistochemistry,IF Microscopy,Western Blot
PurityAnti-GFP IgY was prepared from egg yolks by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins
Concentration1.17 mg/ml
FormatLiquid (sterile filtered)
ReactivityGFP, eGFP, rGFP
TargetGFP
Antibody TypePrimary
StorageStore GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Note: Polyclonal anti-GFP is designed to detect GFP and its variants. Anti-GFP Antibody has been tested by ELISA, dot blot, and western and is suitable in immunofluorescence. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) A rise-to-threshold process for a relative-value decisionNatureJuly 1, 2023Vijayan V, Wang F, Wang K, Chakravorty A, Adachi A, Akhlaghpour H, Dickson BJ, Maimon G.Sigma-Aldrich), 1:10,000 rabbit anti-vGluT78 (gift from A. DiAntonio), 1:1,000 chicken anti-GFP (no. 600-901-215, Rockland) and 1:30 mouse anti-Bruchpilot (no. nc82, Developmental Studies Hybridoma Bank) for all but

(2) Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopyCell Reports MethodsApril 24, 2023Suva Roy, Depeng Wang, Andra M. Rudzite, Benjamin Perry, Miranda L. Scalabrino, Mishek Thapa, Yiyang Gong, Alexander Sher, Greg D. Fieldand GCaMP expressing cells. The following primary antibodies were used: anti-GFP (1:1000, Rockland, 600-901-215), anti-ChAT (1:500, Millipore Sigma, AB144P), anti-PCP2 (1:500, Santa Cruz Biotechnology, Inc. sc-137064),

(3) Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidneyNature communicationsApril 25, 2022Lamontagne JO, Zhang H, Zeid AM, Strittmatter K, Rocha AD, Williams T, Zhang S, Marneros AG.anti-cleaved caspase-3 (Asp175) (Cell Signaling Technology Cat# 9664, RRID:AB_2070042), chicken anti-GFP (Rockland Cat# 600-901-215, RRID:AB_1537402; 1:200 dilution), rabbit anti-GFP (Thermo Fisher Scientific Cat# A-11122, RRID:AB_221569),

(4) Erebosis, a new cell death mechanism during homeostatic turnover of gut enterocytesPLoS biologyApril 1, 2022Ciesielski HM, Nishida H, Takano T, Fukuhara A, Otani T, Ikegawa Y, Okada M, Nishimura T, Furuse M, Yoo SK.for 30 minutes at RT, the samples were incubated with chicken polyclonal anti-GFP antibody (1:2,000; #600-901-215; Rockland Immunochemicals, Limerick, PA) for overnight at RT. The samples were washed 3 times with 0.05%

(5) A biophysical account of multiplication by a single neuronNatureMarch 1, 2022Groschner LN, Malis JG, Zuidinga B, Borst A.

(6) 4D imaging analysis of the aging mouse neural stem cell niche reveals a dramatic loss of progenitor cell dynamism regulated by the RHO-ROCK pathwayStem cell reportsFebruary 8, 2022Zhao X, Fisher ES, Wang Y, Zuloaga K, Manley L, Temple S.h. Primary antibodies were incubated overnight at 4°C; for NSCs: chicken anti-GFP (1:100; Rockland, 600-901-215), rat anti-GFAP (1:500; Invitrogen, 13-0300) and for TAPs: rabbit anti-MASH1 (ASCL1) (1:100; Abcam,

(7) Building an allocentric travelling direction signal via vector computationNatureJanuary 1, 2022Lyu C, Abbott LF, Maimon G.

(8) Experience-dependent myelination following stress is mediated by the neuropeptide dynorphinNeuronNovember 17, 2021Osso LA, Rankin KA, Chan JR.,Thermo Fisher Scientific,"Cat # G10362, RRID:AB_2536526" "Chicken polyclonal anti-GFP, 1:1000",Rockland Immunochemicals,"Cat # 600-901-215, RRID:AB_1537402" "Rabbit polyclonal anti-OLIG2, 1:1000",EMD Millipore,"Cat # AB9610, RRID:AB_570666" "Mouse

(9) Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexesPLoS geneticsApril 1, 2021Li Y, Gong H, Zhan R, Ouyang S, Park KT, Lutkenhaus J, Du S.

(10) Oxytocin Influences Male Sexual Activity via Non-synaptic Axonal Release in the Spinal CordCurrent biology : CBJanuary 11, 2021Oti T, Satoh K, Uta D, Nagafuchi J, Tateishi S, Ueda R, Takanami K, Young LJ, Galione A, Morris JF, et al.above, the sections were incubated with the primary chicken antiserum against GFP (1:2,000 dilution) (600-901-215; Rockland, Gilbertsville, PA, RRID: AB_1537402). After rinsing, the sections were incubated with biotinylated

(11) The Extracellular Superoxide Dismutase Sod5 From Fusarium oxysporum Is Localized in Response to External Stimuli and Contributes to Fungal PathogenicityFrontiers in plant scienceJanuary 1, 2021Wang Q, Pokhrel A, Coleman JJ.

(12) Competing Protein-RNA Interaction Networks Control Multiphase Intracellular OrganizationCellApril 16, 2020Sanders DW, Kedersha N, Lee DSW, Strom AR, Drake V, Riback JA, Bracha D, Eeftens JM, Iwanicki A, Wang A, et al.

(13) let-7-Complex MicroRNAs Regulate Broad-Z3, Which Together with Chinmo Maintains Adult Lineage Neurons in an Immature StateG3 (Bethesda, Md.)April 9, 2020Wu YC, Chawla G, Sokol N.

(14) Lrig1 expression prospectively identifies stem cells in the ventricular-subventricular zone that are neurogenic throughout adult lifeNeural developmentMarch 17, 2020Nam HS, Capecchi MR.

(15) GABAergic Restriction of Network Dynamics Regulates Interneuron Survival in the Developing CortexNeuronJanuary 8, 2020Duan ZRS, Che A, Chu P, Modol L, Bollmann Y, Babij R, Fetcho RN, Otsuka T, Fuccillo MV, Liston C, et al.

(16) Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelopeJournal of molecular cell biologyDecember 23, 2019Liu W, Zheng F, Wang Y, Fu C.

(17) Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum RedistributionToxinsJuly 17, 2019Brito C, Mesquita FS, Bleck CKE, Sellers JR, Cabanes D, Sousa S.

(18) Novel window for cancer nanotheranostics: non-invasive ocular assessments of tumor growth and nanotherapeutic treatment efficacy in vivoBiomedical optics expressJanuary 1, 2019Goswami M, Wang X, Zhang P, Xiao W, Karlen SJ, Li Y, Zawadzki RJ, Burns ME, Lam KS, Pugh EN Jr.

(19) Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in DrosophilaNature communicationsOctober 12, 2018Li MT, Cao LH, Xiao N, Tang M, Deng B, Yang T, Yoshii T, Luo DG.

(20) Visual Projection Neurons Mediating Directed Courtship in DrosophilaCellJuly 26, 2018Ribeiro IMA, Drews M, Bahl A, Machacek C, Borst A, Dickson BJ.

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GFP Antibody from Rockland Immunochemicals, Inc.

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Specifications/Features

Rockland Immunochemicals, Inc.

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GFP Antibody from Rockland Immunochemicals, Inc.

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