Fig 2: Staining of the isolated cells with fluorophore-conjugated specific antibodies.Exemplary immunostainings of the cells isolated from liver specimen (H22-13, see Table 1). (A) The cells labeled with anti-Alb (left panel) and anti-A1AT (right panel) antibodies were found predominantly in the P2 population (97.5% and 86.2% of the P2, respectively) and identified as hepatocytes. (B) Nonparenchymal liver cells were stained with anti-CD45 (populations P3 = 80.3% and P4 = 74.1%, left panel) and anti-CD14 antibodies (population P4 = 43.4%, right panel) (see Table 2 for the list of antibodies). Alb- and A1AT-positive cells–green dots; CD45-positive cells–pink dots; CD14-positive cells–blue dots. Abbr.: Alb, albumin; A1AT, a-1-antitrypsin; FSC, forward scatter; SSC, side scatter.