Fig 1: Characterisation of the antibodies used in this study.(a) E-cadherin, (b) EMP1, (c) 5T4, (d) N-cadherin and (e) CD44 expression assessed using (i) western blot and (ii) immunofluorescence microscopy analysis. Anti-E-cadherin antibody 610181 (BD Biosiences, UK) assessed using A549 cell lysates and normal oral epithelium biopsy; Anti-EMP1 antibody ab173224 (Abcam Plc, UK) assessed using MCF7 cell lysates and normal oral epithelium biopsy; Anti-5T4 antibody ab134162 (Abcam Plc, UK) was assessed using BicR56 cell lysates and normal oral epithelium biopsy; Anti-N-cadherin antibody ab76057 (Abcam Plc, UK) was assessed using A549 cell lysates and T4 OSCC biopsy; Anti-CD44 antibody ab40983 (Abcam Plc, UK) was assessed using MB-MDA-231 cell lysates and normal oral epithelium biopsy. Insets show appropriate negative control Ab staining. Green–antigen staining; Blue shows DAPI nuclear stain.
Supplier Page from Abcam for Anti-N Cadherin antibody