Fig 1: Locally Synthesized Rps4x/eS4 Is Required to Maintain Ribosome Function in Axons(A) Diagram (left) and image of RGC axons after FITC-morpholino introduction in a microfluidic chamber.(B–F) Images (B, D, and E) and QIF plots (bars, average, 95% CI, and distribution of normalized levels) (C and F) of Rps3a/eS1, Rps4x/eS4, Rpl17/uL22, and puromycin immunostaining in axons treated with control morpholinos (Cont.) or with morpholinos against rps3a (eS1) (B and C) or against rps4x (eS4) (D–F), with Welch t test (*p = 0.030, C; 0.015, F; ***p = 0.0004; n.s., not significant).(G) qRT-PCR quantification, normalized to control MO, of 18S rRNA in axonal samples treated with control or rps4x (eS4) morpholinos (n.s., not significant in Mann-Whitney U test).