CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free NBP2-36440 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemCRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNBP2-36440
Quantity0.1 ml (also 0.025 ml)
ApplicationsWestern Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Immunoblotting, Immunohistochemistry Whole-Mount
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
FormatPBS
ReactivityBacteria
TargetCRISPR-Cas9
IsotypeIgG1 Kappa
Clone7A9-3A3
HostMouse
ImmunogenThis CRISPR-Cas9 antibody (7A9-3A3) - N-Terminus was raised against Recombinant Cas9 within the N-terminal region of Streptococcus pyogene. [Uniprot: Q99ZW2]
ClonalityMonoclonal
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
PurityProtein G purified
Research AreaGene Editing, Gene Knockouts, Gene Therapy
ValidationKnockout Validated
Molecular Weight158.4 kDa
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Specificity: This CRISPR-Cas9 antibody (7A9-3A3) - N-Terminus is specific toCas9 protein from Streptococcus pyogene serotype M1

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) A HUWE1 defect causes PARP inhibitor resistance by modulating the BRCA1-∆11q splice variantOncogeneSeptember 1, 2023Pettitt SJ, Shao N, Zatreanu D, Frankum J, Bajrami I, Brough R, Krastev DB, Roumeliotis TI, Choudhary JS, Lorenz S, et al.

(2) MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cellsCell reportsMay 30, 2023Zelceski A, Francica P, Lingg L, Mutlu M, Stok C, Liptay M, Alexander J, Baxter JS, Brough R, Gulati A, et al.

(3) ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcomaNature communicationsApril 7, 2023Tanaka M, Chuaychob S, Homme M, Yamazaki Y, Lyu R, Yamashita K, Ae K, Matsumoto S, Kumegawa K, Maruyama R, et al.Primary antibodies used were ASPSCR1 (1:1000, Sigma-Aldrich, HPA026749), Cas9 (1:1000, Novus Biologicals, NBP2-36440), c-Myc (1:1000, Cell Signaling, 5605), CCBE1 (1:1000, Affinity Biosciences, DF10092), SYNGR1 (1:1000,

(4) Canonical cellular stress granules are required for arsenite-induced necroptosis mediated by Z-DNA-binding protein 1Science signalingMarch 14, 2023Szczerba M, Johnson B, Acciai F, Gogerty C, McCaughan M, Williams J, Kibler KV, Jacobs BL.

(5) Secreted PGK1 and IGFBP2 contribute to the bystander effect of miR-10b gene editing in gliomaMolecular Therapy - Nucleic AcidsMarch 14, 2023Yanhong Zhang, Rosalia Rabinovsky, Zhiyun Wei, Rachid El Fatimy, Evgeny Deforzh, Bai Luan, Leonid Peshkin, Erik J. Uhlmann, Anna M. Krichevskyfor 1 h. The cells were incubated with primary antibodies (mouse anti-CRISPR Cas9, Novus Biologicals, NBP2-36440, 1:200 dilution) overnight at 4°C and further incubated with the secondary antibody (goat anti-mouse

(6) Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 proteineLifeMarch 10, 2023Zhang C, Schekman R.,OriGene Technologies,Cat# TA351394,WB (1:1000)IF (1:300) Antibody,Anti-Cas9 (mouse monoclonal),Novus Biologicals,Cat# NBP2-36440,WB (1:1000) Recombinant DNA reagent,pUCOE- EF1a-dCas9-BFP-KRAB (plasmid),"Dr. Jonathan Weissman (Whitehead

(7) Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancerNature cancerDecember 1, 2022Cappellesso F, Orban MP, Shirgaonkar N, Berardi E, Serneels J, Neveu MA, Di Molfetta D, Piccapane F, Caroppo R, Debellis L, et al.

(8) A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor ImmunityCancer discoveryOctober 5, 2022Abrego J, Sanford-Crane H, Oon C, Xiao X, Betts CB, Sun D, Nagarajan S, Diaz L, Sandborg H, Bhattacharyya S, et al.

(9) Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availabilityMolecular cellAugust 4, 2022Unlu G, Prizer B, Erdal R, Yeh HW, Bayraktar EC, Birsoy K.RRID:AB_2213813 ALAS1,Proteintech,Cat#16200-1-AP; RRID:AB_2878227 CRISPR-Cas9,Novus Biologicals,Cat#NBP2-36440; Clone 7A9-3A3 TFRC (CD71),Cell Signaling Technology,Cat#13113S; RRID:AB_2715594 GFP (for immunofluorescence),Aves Labs

(10) A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cellsMolecular therapy. Nucleic acidsJune 14, 2022Park SB, Uchida T, Tilson S, Hu Z, Ma CD, Leek M, Eichner M, Hong SG, Liang TJ.manufacturer’s instruction. The following antibodies were purchased commercially: anti-Cas9 (Cat#: NBP2-36440; Novus Biologicals, Centennial, CO, USA), anti-p53 (Cat#: NBP2-29453, Novus Biologicals), anti-PTEN

(11) Antispacer peptide nucleic acids for sequence-specific CRISPR-Cas9 modulationNucleic acids researchJune 10, 2022Economos NG, Quijano E, Carufe KEW, Perera JDR, Glazer PM.

(12) Endothelial Retargeting of AAV9 In VivoAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2022Bozoglu T, Lee S, Ziegler T, Jurisch V, Maas S, Baehr A, Hinkel R, Hoenig A, Hariharan A, Kim CI, et al.

(13) CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem CellsMethods in molecular biology (Clifton, N.J.)January 1, 2022Sakashita A, Ariura M, Namekawa SH.

(14) Genetically edited hepatic cells expressing the NTCP-S267F variant are resistant to hepatitis B virus infectionMolecular therapy. Methods & clinical developmentDecember 10, 2021Uchida T, Park SB, Inuzuka T, Zhang M, Allen JN, Chayama K, Liang TJ.proteins were used for the analysis: Cas9 (1:100 dilution; Novus Biologicals, Centennial, CO, cat. #NBP2-36440) and actin (1:100 dilution; Abcam, Cambridge, UK, cat. #ab8227). Sequencing analysis and genotyping

(15) Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2Life science allianceNovember 1, 2021Bailey ML, Tieu D, Habsid A, Tong AHY, Chan K, Moffat J, Hieter P.GeneTex, STAG1 (GTX129912) and ACO1 (GTX128976); from Millipore, RAD21 (05-908); from Novus, CAS9 (NBP2-36440); from Santa Cruz, STAG2 (sc81852), IRP2 (sc33682), and TFRC (sc65582); from Sigma-Aldrich, FLAG M2

(16) Genetic Determinants of EGFR-Driven Lung Cancer Growth and Therapeutic Response In VivoCancer DiscoveryJuly 1, 2021Giorgia Foggetti et al.(1:300, CST, 9664), anti-mKate (1:2500, Evrogen, AB233), anti-Cas9 (1:500, Novus Biologicals, 7A9–3A3, NBP2–36440), anti-β-catenin (1:500, CST, 8814), anti-Rbm10 (1:200, abcam, AB224149), and anti-Keap1 (1:500, abcam,

(17) A Cas12a-based gene editing system for Phytophthora infestans reveals monoallelic expression of an elicitorMolecular plant pathologyJune 1, 2021Ah-Fong AMV, Boyd AM, Matson MEH, Judelson HS.using chemiluminescence, and digitally imaged. Primary antibodies were against Cas9 (Novus Biologicals NBP2‐36440), GFP (Novus Biologicals NB100‐56401), or LbCas12a (Millipore Sigma MABE1824). Microscopy was performed

(18) Trib1 promotes acute myeloid leukemia progression by modulating the transcriptional programs of Hoxa9BloodJanuary 7, 2021Seiko Yoshino, Takashi Yokoyama, Yoshitaka Sunami, Tomoko Takahara, Aya Nakamura, Yukari Yamazaki, Shuichi Tsutsumi, Hiroyuki Aburatani, Takuro Nakamura(#39133) ChIP ERG Rabbit abcam (ab92513) WB c-Myc Mouse Santa Cruz (sc-40) WB CRISPR-Cas9 Mouse Novus (NBP2-36440) WB GAPDH Mouse HyTest (5G4) WB α-Tubulin Mouse Sigma (T5168) WB *Antibodies were used for Western blotting

(19) Characterization of promoters for adeno-associated virus mediated efficient Cas9 activation in adult Cas9 knock-in murine cochleaeHearing researchSeptember 1, 2020Zhao X, Jin C, Dong T, Sun Z, Zheng X, Feng B, Cheng Z, Li X, Tao Y, Wu H.

(20) Adeno-associated virus vector enables safe and efficient Cas9 activation in neonatal and adult Cas9 knockin murine cochleaeGene therapyAugust 1, 2020Kang W, Zhao X, Sun Z, Dong T, Jin C, Tong L, Zhu W, Tao Y, Wu H.

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7/20/2023Cas9 Antibody for Western Blotting From Novus Biologicals Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (42)

Reviews

CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus - BSA Free from Novus Biologicals, a Bio-Techne Brand

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