Fig 1: IL-34 antibody validation. Results shown employed a rabbit polyclonal to IL-34 (Abcam no. AB74548). Similar results were obtained using mouse monoclonal to IL-34 (AB101443) and sheep polyclonal to IL-34 (R and D Systems, AF5265). (A–C) Immunocytochemistry staining of HEK cells transfected with control plasmid (GFP) (A) or plasmid coding IL-34 (B,C). Specific immunostaining (green) was only detected with IL-34 plasmid. Cells were counterstained with DAPI (blue). Low magnification (B) and higher magnification (C). (D) Western blot showing detected bands using AB74548 antibody on recombinant IL-34 (lane 1), and different IL-34 transfected HEK cell samples (lanes 2–4), and different GFP plasmid transfected HEK cell samples (lanes 5–7). The cell expressed IL-34 had molecular weight of approximately 39 kDa. (E) Western blot showing a single band (45 kDa) detected in human brain samples (Br) but not in microglia (MG) or endothelial cell (EC) samples.
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