Pacific Blue(TM) Annexin V 640917 from BioLegend

Product Specs
ItemPacific Blue(TM) Annexin V
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640917
Quantity25 tests
ApplicationsFlow Cytometry (FC)
Conjugate/TagPacific Blue
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Nameannexin A5
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat

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BioLegend

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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell LinesBIO-PROTOCOLJuly 20, 2023Kateryna Shkarina, Petr Broznumber: G8741)DRAQ7 (BioLegend, catalog number: 424001)Annexin V Pacific Blue (BioLegend, catalog number: 640918)Annexin V FITC (BioLegend, catalog number: 640906)Annexin V Alexa Fluor 647 (BioLegend, catalog number:

(2) The DREAM complex functions as conserved master regulator of somatic DNA-repair capacitiesNature Structural & Molecular BiologyApril 1, 2023Arturo Bujarrabal-Dueso, Georg Sendtner, David H. Meyer, Georgia Chatzinikolaou, Kalliopi Stratigi, George A. Garinis, Björn Schumacherwere incubated in annexin V binding buffer (BioLegend, 422201) with Pacific Blue annexin V (BioLegend, 640917) and 7-AAD (Thermo Fisher Scientific, 00699350) at 4 °C for 15 min. Cells were measured using a MACSQuant VYB

(3) Inhibition of Autophagy Promotes the Elimination of Liver Cancer Stem Cells by CD133 Aptamer-Targeted Delivery of DoxorubicinBiomoleculesNovember 3, 2022Wang Yin, Cuong V. Pham, Tao Wang, Hadi Al Shamaileh, Rocky Chowdhury, Shweta Patel, Yong Li, Lingxue Kong, Yingchu Hou, Yimin Zhu, et al.apoptosis of cells. The cells were trypsinized and stained with annexin V (BioLegend, San Diego, CA, USA; #640918, 1:20 dilution) and 7-AAD (BioLegend, San Diego, CA, USA; #420404, 1:20 dilution) for 15 min at room

(4) Alternative splicing of apoptosis genes promotes human T cell survivaleLifeOctober 20, 2022Davia Blake, Caleb M Radens, Max B Ferretti, Matthew R Gazzara, Kristen W Lynchflow cytometry analysis. Cell survival analysis was measured by flow cytometry through Annexin V-Pacific Blue (Biolegend: 640917) and Propidium Iodide staining (Thermo Fisher: c34557). Caspase-3/7 activity was measured with FAM-FLICA

(5) Functional requirements for a Samd14-capping protein complex in stress erythropoiesiseLifeJune 17, 2022Suhita Ray, Linda Chee, Yichao Zhou, Meg A Schaefer, Michael J Naldrett, Sophie Alvarez, Nicholas T Woods, Kyle J Hewittlike PE–CD71 (R17217), APC- Ter119 (116212), PE-Cy7- Kit (clone 2B8) (105814), Pacific Blue Annexin V (640917) antibodies for 30 min at 4°C (all from Biolegend). Cells were resuspended with either DAPI or Draq7

(6) T cell stimulation remodels the latently HIV-1 infected cell population by differential activation of proviral chromatinPLOS PathogensJune 6, 2022Birgitta Lindqvist, Bianca B. Jütte, Luca Love, Wlaa Assi, Julie Roux, Anders Sönnerborg, Tugsan Tezil, Eric Verdin, J. Peter Svensson(50 ng/sample, Biolegend, Cat# 421301) and Annexin V (5 μl, Pacific Blue conjugated, Biolegend, Cat# 640917) were added and the cells were incubated for 15 min at 4°C in the dark before analysis on a Cytoflex

(7) Combined Inactivation of CTPS1 and ATR Is Synthetically Lethal to MYC-Overexpressing Cancer CellsCancer researchMarch 15, 2022Sun Z, Zhang Z, Wang QQ, Liu JL.instructions. Annexin V/propidium iodide (PI) staining was used to determine apoptosis. Pacific Blue Annexin V (640918) was purchased from Biolegend. PI (ST511) was purchased from Beyotime. Annexin V/PI staining was performed

(8) Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanismseLifeFebruary 3, 2022Wang X, Jiao A, Sun L, Li W, Yang B, Su Y, Ding R, Zhang C, Liu H, Yang X, et al.109814; RRID:AB_389211 Antibody,anti-mouse CD3 Pacific Blue (Armenian Hamster monoclonal),Biolegend,Cat# 640918; RRID:AB_493645,Cat# 640918; RRID:AB_493645 Antibody,anti-mouse CD27 FITC (Armenian Hamster monoclonal),Biolegend,Cat#

(9) The inhibition of ABCB1/MDR1 or ABCG2/BCRP enables doxorubicin to eliminate liver cancer stem cellsScientific ReportsDecember 1, 2021Wang Yin et al.(1:20 dilution), VioBright FITC CD133/1 antibody (1:50 dilution), Pacific Blue Annexin V (Biolegend, #640918, 1:20 dilution), and 7-AAD (Biolegend, #420404, 1:20 dilution) for 15 min at room temperature in the

(10) Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammationImmunityAugust 10, 2021Medina CB, Chiu YH, Stremska ME, Lucas CD, Poon I, Tung KS, Elliott MR, Desai B, Lorenz UM, Bayliss DA, et al.Corp.,Cat# LS004176 7AAD Viability Stain,Thermo Fisher,Cat# A1310 Annexin V Pacific Blue,Biolegend,Cat# 640918 EdU,Thermo Fisher,Cat# A10044 Protease Inhibitors,Calbiochem,Cat# 539131 CFSE,Invitrogen,Cat# C34554 ATP,Sigma-Aldrich,Cat#

(11) Tumor regression and immunity in combination therapy with anti-CEA chimeric antigen receptor T cells and anti-CEA-IL2 immunocytokineOncoImmunologyApril 2, 2021Seung E. Cha, Maciej Kujawski, Paul J. Yazaki, Christine Brown, John E. ShivelyAPC-conjugated antibody (R&D, FAB412181A). Mouse cells were stained with Pacific Blue Annexin V (Biolegend, 640918) or Horizon Fixable Viability Stain 510 (BD, 564406) for cell viability. Flow cytometry was performed

(12) In oxygen-deprived tumor cells ERp57 provides radioprotection and ensures proliferation via c-Myc, PLK1 and the AKT pathwayScientific reportsMarch 30, 2021Ocklenburg T, Neumann F, Wolf A, Vogel J, Göpelt K, Baumann M, Baumann J, Kranz P, Metzen E, Brockmeier U.× 105 cells were incubated with 80 µg/ml PI and 9.6 µg/ml Annexin V conjugated to PacificBlue (No. 640917, Biolegend, San Diego, CA, USA) in Annexin V binding buffer (No. 422201, Biolegend) for 15 min at room

(13) Erythropoietin Promotes Infection Resolution and Lowers Antibiotic Requirements in E. coli- and S. aureus-Initiated InfectionsFrontiers in immunologyJanuary 1, 2021Liang F, Guan H, Li W, Zhang X, Liu T, Liu Y, Mei J, Jiang C, Zhang F, Luo B, et al.(406421[Poly4064]; BioLegend, San Diego, CA, USA), p-Jak2 (Ab219728[E132]; Abcam, Cambridge, UK), annexin V (640918; BioLegend, San Diego, CA, USA), E. coli antibody (GTX40856[polyclonal]; GeneTex, Irvine, CA, USA),

(14) Obesity induces gut microbiota alterations and augments acute graft-versus-host disease after allogeneic stem cell transplantationScience Translational MedicineNovember 25, 2020Lam T. Khuat et al.single-cell suspensions (1 – 5 × 106 cells/ml were stained with Annexin V-Pacific Blue (Biolegend Cat# 640918, San Diego, CA, USA). Cells then were incubated in dark for 15 minutes and stained with PI (Life Technologies,

(15) EZH2-Deficient T-cell Acute Lymphoblastic Leukemia Is Sensitized to CHK1 Inhibition through Enhanced Replication StressCancer DiscoveryJuly 1, 2020Theresa E. León et al.(control), cells were collected and washed with PBS, stained using Pacific Blue-Annexin V (BioLegend; Cat. 640917) and analyzed by flow cytometry using a BD FACSymphonyTM cytometer (BD Biosciences). 10,000 events were

(16) Diaphanous-related formin mDia2 regulates beta2 integrins to control hematopoietic stem and progenitor cell engraftmentNature communicationsJune 23, 2020Mei Y, Han X, Liu Y, Yang J, Sumagin R, Ji P.above, followed by Cy5-Annexin V (Catalog #559934, BD Pharmingen) or Pacific blue-Annexin V (Catalog #640918, BioLegend) staining (1:200 diluted in Annexin V-binding buffer) at room temperature for 15–20 min.

(17) STING Gain-of-Function Disrupts Lymph Node Organogenesis and Innate Lymphoid Cell Development in MiceCell reportsJune 16, 2020Bennion BG, Croft CA, Ai TL, Qian W, Menos AM, Miner CA, Frémond ML, Doisne JM, Andhey PS, Platt DJ, et al.Recombinant murine FLT3-ligand,Peprotech,Cat# 250-31L Promega RNasin Plus Rnase Inhibitor,Promega,Cat# N2611 Annexin V,Biolegend,Cat# 640917 Annexin V,BD Biosciences,Cat# 556421 Critical Commercial Assays,, Mouse IgA ELISA Kit,"Immunology

(18) Disruption of TFIIH activities generates a stress gene expression response and reveals possible new targets against cancerOpen biologyJune 1, 2020Uriostegui-Arcos M, Aguayo-Ortiz R, Valencia-Morales MDP, Melchy-Pérez E, Rosenstein Y, Dominguez L, Zurita M.(Invitrogen, Cat. 65-0865) and Biolegend Pacific Blue Annexin V or FITC Annexin V (BioLegend, Cat. 640918 or Cat. 640906, respectively). Intracellular protein staining was performed as previously described

(19) Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic DevelopmentCell reportsMarch 3, 2020Ramstead AG, Wallace JA, Lee SH, Bauer KM, Tang WW, Ekiz HA, Lane TE, Cluntun AA, Bettini ML, Round JL, et al.H1.2F3,Biolegend,Cat#104508 Rat anti-mouse CD62L Clone MEL-14,Biolegend,Cat#104412 Annexin V,Biolegend,Cat#640918 7-AAD Viability Staining Solution,Biolegend,Cat#420404 Rat anti-mouse CD117 Clone ACK2,Biolegend,Cat#135106 Ultra-LEAF

(20) Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4⁺ T Cell Pathogenicity and Suppresses AutoimmunityCell metabolismFebruary 4, 2020Angiari S, Runtsch MC, Sutton CE, Palsson-McDermott EM, Kelly B, Rana N, Kane H, Papadopoulou G, Pearce EL, Mills KHG, et al.K-12049-D50 Propidium Iodide,Invitrogen,Cat #: P1304MP; CAS: 25535-16-4 Pacific Blue™ Annexin V,BioLegend,Cat #: 640918 PMA,Sigma Aldrich,Cat #: P8139; CAS:16561-29-8 Ionomycin,Sigma Aldrich,Cat #: I0634; CAS: 56092-82-1 Brefeldin A

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1/23/2019Pacific Blue Annexin V Anup KumarI am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This antibody is good for detecting Annexin V on mouse immune cells to ... Read Review

Pacific Blue(TM) Annexin V from BioLegend

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Pacific Blue(TM) Annexin V from BioLegend

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