Anti-GPCR TGR5 antibody ab72608 from Abcam

Product Specs
ItemAnti-GPCR TGR5 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab72608
Antibody TypePrimary
Conjugate/TagUnconjugated
ClonalityPolyclonal
IsotypeIgG
Quantity100 UL
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Gene NameGPBAR1
ReactivityHuman, Mouse
TargetGPCR TGR5

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Insulin alleviates murine colitis through microbiome alterations and bile acid metabolismJournal of Translational MedicineJuly 25, 2023Shuying He, Jiating Li, Zirong Yao, Zixian Gao, Yonghong Jiang, Xueqing Chen, Liang Pengcocktail (Roche, Basel, Switzerland) and phosphatase inhibitor (Roche). An anti-TGR5 primary antibody (ab72608) was purchased from Abcam, and an anti-GAPDH primary antibody was purchased from Zhonshanjinqiao (Wuhan,

(2) Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species-p90RSK-Vascular Endothelial Growth Factor Receptor 3 PathwayCellsFebruary 6, 2023Banerjee P, Kumaravel S, Roy S, Gaddam N, Odeh J, Bayless KJ, Glaser S, Chakraborty S.,Sigma Aldrich,Y4645,AB_1080624 SUMO1,"IP, WB",1:1000,Cell Signaling Technology,4940,AB_2302825 GPCR TGR5,"IF, WB","1:100, 1:1000",Abcam,ab72608,AB_2112165 S1PR2,"IF, WB","1:100, 1:1000",Thermo Fisher Scientific,PA523208,AB_2540734 FXR,"IF, WB","1:100,

(3) GPBAR1 is associated with asynchronous bone metastasis and poor prognosis of hepatocellular carcinomaFrontiers in OncologyJanuary 23, 2023Nan Chen, Jieqing Wang, Lei Zhou, Baiqiang Hu, Yinzhong Chen, Zhuangchen Zhuto incubate specimens to block unspecific antigen binding. The primary antibody for GPBAR1 (Abcam, ab72608, Cambridge, UK) was administrated to incubate the tissues overnight at 1:100 concentrations, followed

(4) Deoxycholic acid induces gastric intestinal metaplasia by activating STAT3 signaling and disturbing gastric bile acids metabolism and microbiotaGut MicrobesDecember 31, 2022Duochen Jin, Keting Huang, Miao Xu, Hongjin Hua, Feng Ye, Jin Yan, Guoxin Zhang, Yun Wangfor 1 h at room temperature and then stained with the indicated primary antibodies against TGR5 (cat. ab72608, 1:100, Abcam), phospho-Stat3 (Tyr705, cat. #9145, 1:100, CST) and KLF5 (cat. 61099, 1:100, Active Motif)

(5) Investigation of the potential role of TGR5 in pancreatic cancer by a comprehensive molecular experiments and the liquid chromatography mass spectrometry (LC–MS) based metabolomicsDiscover OncologyDecember 1, 2022Yangyang Lei, Guoping Li, Jianke Li, Shanshan Gao, Ming Lei, Gaoquan Gong, Changyu Li, Yi Chen, Chenggang Wang, Xiaolin Wangwith 5% skim milk at room temperature for 2 h and then probed with anti-TGR5 antibody (1:1000, Abcam, ab72608) and anti-β-actin (1:2000, Cell Signaling, Rabbit mAb, 4970) overnight at 4℃. After being rinsed with

(6) The gut microbe Bacteroides fragilis ameliorates renal fibrosis in miceNature communicationsOctober 14, 2022Zhou W, Wu WH, Si ZL, Liu HL, Wang H, Jiang H, Liu YF, Alolga RN, Chen C, Liu SJ, et al.dilution 1:2000 for WB), Occludin (66378-1-Ig, Proteintech, mouse, dilution 1:5000 for WB), anti-TGR5 (ab72608, Abcam, rabbit, dilution 1:1000 for WB and 1:100 for IHC), anti-IL-1β (ab254360, Abcam, rabbit, dilution

(7) Abnormal bile acid metabolism is an important feature of gut microbiota and fecal metabolites in patients with slow transit constipationFrontiers in Cellular and Infection MicrobiologyJuly 28, 2022Yadong Fan et al.phosphate buffered saline for 15 min on ice. Antigens of Takeda G protein-coupled receptor (TGR5, Lot: ab72608, Abcam, England) and nuclear farnesoid X receptor (FXR, Lot: bs-12867R, Bioss, China) were unmasked

(8) Iron deficiency linked to altered bile acid metabolism promotes Helicobacter pylori–induced inflammation–driven gastric carcinogenesisJournal of Clinical InvestigationMay 16, 2022Jennifer M. Noto et al.analysis was performed on a gastric cancer TMA using a rabbit polyclonal anti-GPCR TGR5 antibody (Abcam ab72608). The gastric cancer TMA was generated at Vanderbilt University Medical Center with tissues from the

(9) Ileo-Colonic Delivery of Conjugated Bile Acids Improves Glucose Homeostasis via Colonic GLP-1-Producing Enteroendocrine Cells in Human Obesity and DiabetesSSRN Electronic JournalApril 4, 2022Gerardo Calderon et al.(ab23468, Abcam, 1:200, RRID:AB_470325), FXR (PA540755, Invitrogen, 1:100, RRID:AB_2605921), TGR5 (ab72608, Abcam, 1:100, RRID: AB_2112165), or ASBT (sc-27493, Santa Cruz Biotechnology, 1:100, RRID:AB_2188364).

(10) Changes of farnesoid X receptor and Takeda G‑protein coupled receptor 5 following biliary tract external drainage in hemorrhagic shockExperimental and Therapeutic MedicineDecember 21, 2021Lu Wang, Huai-Wu He, Xiang Zhou, Yun Longantibodies were rabbit polyclonal anti-FXR (cat. no. ab28676) and anti-TGR-5 (both 1:500; cat. no. ab72608; both Abcam) and mouse monoclonal anti-β-actin (Santa Cruz Biotechnology, Inc.; cat. no. sc517582) (1:1,000).

(11) Evolutionary metabolic landscape from preneoplasia to invasive lung adenocarcinomaNature communicationsNovember 10, 2021Nie M, Yao K, Zhu X, Chen N, Xiao N, Wang Y, Peng B, Yao L, Li P, Zhang P, et al.room temperature. Membranes were incubated with antibodies including TGR5 (1:1000, Abcam, catalog No: ab72608) and α-Tubulin (1:3000, ABclonal, catalog No: AC012). For detection, HRP-linked anti-rabbit and anti-mouse

(12) Flaxseed Powder Attenuates Non-Alcoholic Steatohepatitis via Modulation of Gut Microbiota and Bile Acid Metabolism through Gut–Liver AxisInternational Journal of Molecular SciencesOctober 8, 2021Chao Yang, Min Wan, Dengfeng Xu, Da Pan, Hui Xia, Ligang Yang, Guiju SunPrimary antibodies used were FXR (1:1000, mAb #72105, Cell Signalling Technology, Beverly, MA), TGR5 (ab72608, Abcam, Cambridge, United Kingdom), CYP7A1 (1:1000, ab138497, RRID: AB_2828001, Abcam, Cambridge, United

(13) TGR5 Expression Is Associated with Changes in the Heart and Urinary Bladder of Rats with Metabolic SyndromeLife (Basel)July 15, 2021Chia-Chen Hsu, Kai-Chun Cheng, Yingxiao Li, Ping-Hao Hsu, Juei-Tang Cheng, Ho-Shan Niufor p-STAT3 (1:1000, phosphor-Y705, ab76315, Abcam), STAT3 (1:1000, ab68153, Abcam), TGR5 (1:1000, ab72608, Abcam), or β-actin (1:5000, A5316, Sigma-Aldrich, St. Louis, MO, USA). The bound primary antibodies

(14) Liver Bile Acid Changes in Mouse Models of Alzheimer’s DiseaseInternational Journal of Molecular SciencesJuly 12, 2021Harpreet Kaur, Drew Seeger, Svetlana Golovko, Mikhail Golovko, Colin Kelly Combsvitamin D receptor (VDR) (cat.no:, ab3508, 1:700), Takeda G-protein coupled receptor 5 (TGR-5) (cat. no: ab72608, 1:1000), Anti-amyloid precursor protein (Y188) (cat. no: ab32136, 1:750) and CYP46A1 (cat.no: ab244241,

(15) TGR5 Regulates Macrophage Inflammation in Nonalcoholic Steatohepatitis by Modulating NLRP3 Inflammasome ActivationFrontiers in ImmunologyFebruary 22, 2021Yong Shi et al.#13120, #14994, #5397, #9177, #56554, Cell Signaling Technology, Danvers, MA, USA), and TGR5, and CD206 (#ab72608, #ab64693, Abcam, Cambridge, MA, USA) were diluted 1:1000 in a primary antibody buffer (Beyotime Biotechnology

(16) TGR5 activation attenuates neuroinflammation via Pellino3 inhibition of caspase-8/NLRP3 after middle cerebral artery occlusion in ratsJournal of neuroinflammationFebruary 2, 2021Liang H, Matei N, McBride DW, Xu Y, Zhou Z, Tang J, Luo B, Zhang JH.incubated overnight at 4 °C with primary antibodies, including: rabbit polyclonal anti-TGR5 (1:100; ab72608; Abcam, Cambridge, MA,USA), mouse monoclonal anti-Pellino3 (1:100; sc-376466; Santa Cruz Biotechnology,

(17) Paeoniflorin Improved Constipation in the Loperamide-Induced Rat Model via TGR5/TRPA1 Signaling-Mediated 5-Hydroxytryptamine SecretionEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2021Zhan Y, Wen Y, Zhang LL, Shen XL, Chen XH, Wu XH, Tang XG.was used to block the membranes. The corresponding protein antibodies were as follows: TGR5 (Abcam, ab72608; 1/1000), transient receptor potential ankyrin 1 (TRPA1; Alomone Labs, ACC-037; 1/1000), phospholipase

(18) TGR5-HNF4α axis contributes to bile acid-induced gastric intestinal metaplasia markers expressionCell Death DiscoveryDecember 1, 2020Zhen Ni et al.Cell Signaling Technology (Danvers, MA, USA). Primary antibodies against human KLF4 (ab215036, 1:1000), TGR5 (ab72608, 1:1000), mucin 5AC (MUC5AC) (ab24071, 1:1000), and mucin 13 (MUC13) (ab235450, 1:1000) were from Abcam

(19) Ileo-colonic delivery of conjugated bile acids improves glucose homeostasis via colonic GLP-1-producing enteroendocrine cells in human obesity and diabetesEBioMedicineMay 1, 2020Calderon G, McRae A, Rievaj J, Davis J, Zandvakili I, Linker-Nord S, Burton D, Roberts G, Reimann F, Gedulin B, et al.(ab23468, Abcam, 1:200, RRID:AB_470325), FXR (PA540755, Invitrogen, 1:100, RRID:AB_2605921), TGR5 (ab72608, Abcam, 1:100, RRID:AB_2112165), or ASBT (sc-27493, Santa Cruz Biotechnology, 1:100, RRID:AB_2188364).

(20) A dysregulated bile acid-gut microbiota axis contributes to obesity susceptibilityEBioMedicineMay 1, 2020Wei M, Huang F, Zhao L, Zhang Y, Yang W, Wang S, Li M, Han X, Ge K, Qu C, et al.(Sakura, USA) and cut into frozen intestinal sections. The slides were treated with TGR5 antibody (ab72608, Abcam, RRID:AB_2112165) and GLP-1 (ab23468, Abcam, RRID:AB_470325), and were then exposed to Alexa

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Anti-GPCR TGR5 antibody from Abcam

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Anti-GPCR TGR5 antibody from Abcam

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