Fig 1: Trichoplein acts as the activator of Aurora A. (A–C) Western blot and quantification of TCHP and p-Aurora A (T288) after stromal cells were transfected with siTCHP for 24 h then in vitro decidualization for 48 h. (D) ARL13B immunofluorescence after stromal cells were transfected with siTCHP for 24 h then in vitro decidualization for 48 h. Scale bars, 5 µm. (E,F) Ciliated cells and cilia length were analyzed after stromal cells were transfected with siTCHP for 24 h then in vitro decidualization for 48 h. (G–I) Relative mRNA levels of TCHP, IGFBP1, and PRL after stromal cells were transfected with siTCHP for 24 h then in vitro decidualization for 48 h. In (A,D), the representative images of three biologically independent experiments are shown. In (F), the results are pooled from three independent experiments and presented as violin plots. The thick bar indicates the median and the dotted lines the first and third quartiles. In (B,C,E–I), data are presented as means ± SD from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by two-tailed Student’s t test for comparing two groups or one-way ANOVA test for comparing more than two groups.