Fig 2: The correlations of SPA17 expression and the infiltration levels of CD4+ T cells, CAF, progenitor, Endo, Eos, HSC, Tfh, ?dT, NKT, regulatory T cells (Tregs), B cells, neutrophils, monocytes, macrophages, dendritic cells, NK cells, Mast cells and CD8+ T cells in cancers. Positive correlation in red and negative correlation in blue.

Fig 3: (A) The Spearman correlation heatmap shows the correlation between the expression of SPA17 and 47 kinds of immune regulators in pan-cancer. Red represents positive correlation and blue represents negative correlation. (B) Correlations between SPA17 expression and tumor mutation burden in pan-cancer. (C) Correlations between SPA17 expression and microsatellite instability in pan-cancer. (D) Kaplan-Meier curve of low and high-SPA17 subgroup in IMvigor210 cohort (anti-PD-L1), and the proportion of tumors (including kidney cancer) in the low-SPA17 and high-SPA17 subgroups in the IMvigor210 cohort who responded to PD-1 therapy. (E) Kaplan-Meier curve of GSE91061 (anti-PD-L1 melanoma) in the low and high-SPA17 patient groups, and the proportion of melanoma patients in the GSE91061 low and high-SPA17 subgroups that responded to anti-PD-1 therapy. The labelled asterisk indicated the statistical p-value (*p < 0.05, **p < 0.01, ***p < 0.001).

Fig 4: The hallmarks gene set enrichment analysis (GSEA) of SPA17 in pan-cancer. The size of the circle represents the false discovery rate (FDR) value of each cancer enrichment item, and the color represents the normalized enrichment score (NES) of each enrichment item.

Fig 5: (A) Based on the univariate Cox regression and Kaplan-Meier models, summary of the correlation between SPA17 expression and overall survival (OS), disease-specific survival (DSS), disease-free interval (DFI) and progression-free interval (PFI). Red indicates that SPA17 is a risk factor for the prognosis of cancer patients, while blue represents a protective factor. Only p-values < 0.05 are shown. (B) The forest plot showed the prognostic role of SPA17 in cancers by univariate Cox regression. The red type of cancer represents SPA17 as a statistically significant risk factor, and blue represents a protective factor. (C–F) Kaplan-Meier overall survival curves of SPA17 in BRCA (C), KIRC (D), LGG (E) and TGCT (F).