Fig 1: Glyco-code subtypes are associated with EMT and coexist in PDAC tumours.a Graphical representation of the top five gene sets enriched in each cluster. b GSEA representation of the gene set ‘HALLMARK Epithelial to Mesenchymal transition’. c EMT Score of both glyco-clusters A and B. Statistics: Mann–Whitney test (*p = 0.05, **p = 0.01, ***p = 0.001, ****p = 0.0001). d Analysis of single-cell RNA-seq identify clusters of tumour cells associated with the Classical and Basal molecular subtypes defined by Moffit et al. Data presented as violin plots with boxplots indicating the median, 25th and 75th percentiles (hinge) and whiskers represent 1.5 times the interquartile range. e Quantification of the Classical (blue) and Basal (beige-orange) tumour clusters in different patients. f Expression of EMT markers E-Cadherin (CDH1), Vimentin (VIM) and Fucosylation-related genes in different clusters in scRNA-seq. Correlation of EMT scores g O-glycosylation h and Fucosylation pathway i with Classical score. j Our results suggest defined changes in glycosylation during EMT in PDAC.