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Antibodies
Anti-CTCF antibody
Anti-CTCF antibody from Abcam
Be the first to write a review!
Citations:
(66)
Supplier Page
Supplier Page from
Abcam for
Anti-CTCF antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-CTCF antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab70303
Antibody Type
Primary
Isotype
IgG
Host
Rabbit
Clonality
Polyclonal
Quantity
100 UL
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Conjugate/Tag
Unconjugated
Format
ab70303 was affinity purified using an epitope specific to CTCF immobilized on solid support
Concentration
0.2 mg/ml
Research Area
Epigenetics
Gene Name
CTCF
Reactivity
Human, Mouse
Target
CTCF
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (66)
(1)
Mitotic bookmarking by SWI/SNF subunits
Nature
June 1, 2023
Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF,
et al
.
H3K9me3 (
Active Motif
,
39161
); H4K20me3 (
Active Motif
,
39671
);
SOX2
(R&D systems,
AF2018
), CTCF (
Abcam
,
ab70303
).
Immunoprecipitates
were washed in 150 and 500 mM NaCl wash buffer as well as a lithium chloride wash.
(2)
A DNA tumor virus globally reprograms host 3D genome architecture to achieve immortal growth
Nature Communications
March 22, 2023
Chong Wang, Xiang Liu, Jun Liang, Yohei Narita, Weiyue Ding, Difei Li, Luyao Zhang, Hongbo Wang, Merrin Man Long Leong, Isabella Hou,
et al.
ZHT, P3HR1 ZHT cells, and
virus
induction were previously described81,82. 1μg anti- CTCF (
Abcam
Cat: #
ab70303
) antibody was used for CUT & Run; 1μg anti-H3K27ac (
Abcam
Cat: #
ab4729
) antibody was used for CUT &
(3)
The aberrant epigenome of DNMT3B-mutated ICF1 patient iPSCs is amenable to correction, with the exception of a subset of regions with H3K4me3- and/or CTCF-based epigenetic memory
Genome Research
February 24, 2023
Varsha Poondi Krishnan, Barbara Morone, Shir Toubiana, Monika Krzak, Salvatore Fioriniello, Floriana Della Ragione, Maria Strazzullo, Claudia Angelini, Sara Selig, Maria R. Matarazzo
(
Merck Millipore
16661
) and anti-
DNMT3B
antibody (
Diagenode
C15410218
) and anti-CTCF antibody (
Abcam
ab70303
). Decrosslinking from beads was performed in a 1% SDS solution containing Tris-HCl pH 8 and 10 mM EDTA
(4)
A Mediator-cohesin axis controls heterochromatin domain formation
Nature Communications
December 1, 2022
Judith H. I. Haarhuis, Robin H. van der Weide, Vincent A. Blomen, Koen D. Flach, Hans Teunissen, Laureen Willems, Thijn R. Brummelkamp, Benjamin D. Rowland, Elzo de Wit
Santa Cruz
),
HSP90
(F-8,
sc-13119
,
Santa Cruz
),
MED12
(
A300-774A
, Bethyl), CCNC (
ab85927
,
Abcam
),
CTCF
(
ab70303
,
Abcam
), Actin (
ab6276
,
Abcam
) and Tubulin (
T5168
,
Sigma-Aldrich
). All primary antibodies were used
(5)
Analysis of sub-kilobase chromatin topology reveals nano-scale regulatory interactions with variable dependence on cohesin and CTCF
Nature Communications
December 1, 2022
Abrar Aljahani, Peng Hua, Magdalena A. Karpinska, Kimberly Quililan, James O. J. Davies, A. Marieke Oudelaar
antibody, rabbit polyclonal,
Abcam
,
ab154769
,
1:1000
; CTCF: anti-CTCF antibody, rabbit polyclonal,
Abcam
,
ab70303
,
1:1000
), and the second piece with lower protein ladder size for the
incubation
with the loading control
(6)
3D‐Epigenomic Regulation of Gene Transcription in Hepatocellular Carcinoma
Adv Genet (Hoboken)
December 1, 2022
Yuliang Feng, Ping Wang, Liuyang Cai, Meixiao Zhan, Fan He, Jiahui Wang, Yong Li, Eva Gega, Wei Zhang, Wei Zhao,
et al.
and immunoprecipitation
was performed using anti‐RNAPII (BioLegend, cat.# 8WG16)/anti‐CTCF antibody (
Abcam
, cat.#
ab70303
) coated protein G beads (
Life Technologies
, cat. no.
10009D
). Then on‐beads A‐tailing was performed
(7)
The 3D enhancer network of the developing T cell genome is shaped by SATB1
Nature communications
November 14, 2022
Zelenka T, Klonizakis A, Tsoukatou D, Papamatheakis DA, Franzenburg S, Tzerpos P, Tzonevrakis IR, Papadogkonas G, Kapsetaki M, Nikolaou C,
et al
.
long isoform (targeting only the extra peptide present in the long isoform54), 7 µg anti-CTCF (
Abcam
,
ab70303
) and 2 µg anti-H3K27ac (
Abcam
,
ab4729
). Antibody-coupled beads were incubated with
chromatin
at 4 °C
(8)
Multimodal regulatory elements within a hormone-specific super enhancer control a heterogeneous transcriptional response
Molecular cell
February 17, 2022
Hoffman JA, Trotter KW, Day CR, Ward JM, Inoue K, Rodriguez J, Archer TK.
RRID:AB_2732926" RAD21,
Abcam
,"
ab992
, RRID:AB_2176601" SMC3,
Abcam
,"
ab9263
, RRID:AB_307122" CTCF,
Abcam
,"
ab70303
, RRID:AB_1209546" E2F1 (C20),
Santa Cruz
,"
sc-193
, RRID:AB_631394" E2F1,
Millipore Sigma
,"
05-379
, RRID:AB_2096772" ,, "Chemicals,
(9)
Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells
Cellular and molecular life sciences : CMLS
January 3, 2022
Tian GG, Zhao X, Hou C, Xie W, Li X, Wang Y, Wang L, Li H, Zhao X, Li J,
et al
.
anti-H3K4me3 (
ab8580
;
Abcam
), anti-H3K27ac (
ab4729
;
Abcam
), anti-H3K27me3 (
07-449
;
Millipore
), and anti-CTCF (
ab70303
;
Abcam
). After reverse crosslinking,
immunoprecipitated
DNA and input DNA were end-repaired and adapters
(10)
Large parental differences in chromatin organization in pancreatic beta cell line explaining diabetes susceptibility effects
Nature Communications
December 1, 2021
Xing Jian, Gary Felsenfeld
carried out according to the manufacturer’s instructions with an anti-CTCF antibody (
Abcam
, catalog no.
ab70303
), and 3 µL antibody was used in each experiment (
1:33
dilution). The crosslink was reversed by heating,
(11)
Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes
Virology Journal
December 1, 2021
Xin Li et al.
H3K27me3 (
ab6002
) and IgG (
ab46540
) were from
Abcam
. CTCF polyclonal antibodies were bought from
Abcam
(
ab70303
), CTCF monoclonal antibodies were from
Millipore
(
17-10044
).
Alexa Fluor 594 Goat Anti-Mouse IgG (H + L) Antibody
(12)
Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture
Nature Communications
December 1, 2021
Hye Ji Cha et al.
antibodies diluted
1:1000
were used: Esco2 (bethyl, A301-689A),
Matr3
(Abcam,
ab84422
), CTCF (
Abcam
,
ab70303
), Rad21 (
Abcam
,
ab992
), Smc3 (
Abcam
,
ab9263
), Mbd1 (
Abcam
,
ab187734
). Uncropped blots are provided in
(13)
3'HS1 CTCF binding site in human β-globin locus regulates fetal hemoglobin expression
eLife
September 29, 2021
Himadewi P, Wang XQD, Feng F, Gore H, Liu Y, Yu L, Kurita R, Nakamura Y, Pfeifer GP, Liu J,
et al
.
BioMag Plus Concanavalin A (BangsLabs, Inc). Cells were permeabilized and incubated with CTCF antibody (
ab70303
,
Abcam
) overnight. After washing away unbound antibody, pA-MNase (a gift from Dr. Steven Henikoff) was
(14)
Acute depletion of CTCF rewires genome-wide chromatin accessibility
Genome biology
August 24, 2021
Xu B, Wang H, Wright S, Hyle J, Zhang Y, Shao Y, Niu M, Fan Y, Rosikiewicz W, Djekidel MN,
et al
.
1:10,000
),
USF1
(
Proteintech
,
22327–1-AP
,
1:2000
),
USF2
(
Novus
,
NBP1-92649
,
1:2000
), and CTCF (
Abcam
,
ab70303
,
1:1000
) at 4 °C for 12 h with gentle shaking. The
membranes
were washed three times for 30 min and
(15)
The role of CTCF in the organization of the centromeric 11p15 imprinted domain interactome
Nucleic Acids Research
June 21, 2021
Natali S Sobel Naveh, Daniel F Deegan, Jacklyn Huhn, Emily Traxler, Yemin Lan, Rosanna Weksberg, Arupa Ganguly, Nora Engel, Jennifer M Kalish
parameters: PIP 75, DF 5%, CPB 200, 6°C setpoint for 16 min total, then precipitated using anti-CTCF (
ab70303
;
Abcam
) and rabbit IgG. To quantify CTCF occupancy,
qPCR
was performed with primers listed in Supplementary
(16)
Regulation of host and viral promoters during human cytomegalovirus latency via US28 and CTCF
Journal of General Virology
May 27, 2021
Elizabeth G. Elder, Benjamin A. Krishna, Emma Poole, Marianne Perera, John Sinclair
blocked in 5 % milk in tris buffered saline (TBS) with 0.1 % Tween-20. Antibodies used: anti-CTCF (
Abcam
ab70303
), anti-GAPDH (
Abcam
ab9485
), anti-beta actin (
Abcam
ab6276
). Chromatin immunoprecipitation (ChIP)
Chromatin immunoprecipitation
(17)
Sp1 Targeted PARP1 Inhibition Protects Cardiomyocytes From Myocardial Ischemia–Reperfusion Injury via Downregulation of Autophagy
Frontiers in Cell and Developmental Biology
May 25, 2021
Yifeng Xu, Boqian Wang, Xiaoxiao Liu, Yunfei Deng, Yanqi Zhu, Feng Zhu, Yanyan Liang, Hongli Li
1:10
, Cambridge, United Kingdom), YYI (
Abcam
,
ab109237
,
1:10
, Cambridge, United Kingdom), CTCF (
Abcam
,
ab70303
,
1:10
, Cambridge, United Kingdom), β-actin (
Abcam
,
ab8227
,
1:5000
, Cambridge, United Kingdom), GAPDH
(18)
Noncoding RNA processing by DIS3 regulates chromosomal architecture and somatic hypermutation in B cells
Nature Genetics
February 1, 2021
Brice Laffleur et al.
(0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-Cl (pH 8.0) and 167 mM NaCl). CTCF (
Abcam
,
ab70303
) and RAD21 (
Abcam
,
ab992
) antibodies were added to
chromatin
, and samples were rotated at 4 °C for 16
(19)
Generation of onco-enhancer enhances chromosomal remodeling and accelerates tumorigenesis
Nucleic Acids Research
December 2, 2020
Peiwei Chai et al.
in this study: anti-NTS (
Abcam
,
ab233107
, USA), anti-EP300 (
Abcam
,
ab10485
, USA), anti-CTCF (
Abcam
,
ab70303
, USA), anti-SMC1 (
Abcam
,
ab9262
, USA) and GAPDH (Sigma-Aldrich).
Co-IP
Co-IP
was performed according
(20)
Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen
eLife
October 1, 2020
Zhang H, Zhang Y, Zhou X, Wright S, Hyle J, Zhao L, An J, Zhao X, Shao Y, Xu B,
et al
.
AB_11007053" Antibody,Anti-
USF1
,
Proteintech
,
22327–1-AP
,"IB (
1: 2,000
) AB_2060867" Antibody,Anti-CTCF (Rabbit polyclonal),
Abcam
,
ab70303
,"IB (
1:1,000
) AB_1209546" Antibody,Anti-MYC (Rabbit polyclonal),
Cell Signaling Technology
,
9402
,IB (
1:1000
)
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Reviews
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Anti-CTCF antibody from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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