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Antibodies
Anti-TRPV4 antibody
Anti-TRPV4 antibody from Abcam
Be the first to write a review!
Citations:
(61)
Supplier Page
Supplier Page from
Abcam for
Anti-TRPV4 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-TRPV4 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab39260
Antibody Type
Primary
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Isotype
IgG
Host
Rabbit
Clonality
Polyclonal
Quantity
100 UG
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Research Area
Neuroscience
Gene Name
TRPV4
Reactivity
Horse, Mouse
Target
TRPV4
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (61)
(1)
The role of TRPV4 in the regulation of retinal ganglion cells apoptosis in rat and mouse
Heliyon
July 1, 2023
Yi Wang, Wei Zhang, Guozheng Xu, Changwei Shi, Xiang Wang, Jianfeng Qu, Hongmei Wang, Chunhua Liu
solution for 30 min, washed with PBS for 3 × 1 min, and a primary antibody-containing TRPV4 (
Abcam
,
ab39260
) was added at 4 °C overnight. A fluorescent-labeled second antibody (a32731) was used for signal detection,
(2)
Dental Pulp Stem Cell-Derived Exosomes Alleviate Mice Knee Osteoarthritis by Inhibiting TRPV4-Mediated Osteoclast Activation
International Journal of Molecular Sciences
March 3, 2023
Yu Fu, Shengjie Cui, Yanheng Zhou, Lixin Qiu
Santa Cruz Biotechnology
), IL-1β (
1:100
,
sc-52012
;
Santa Cruz Biotechnology
, Dallas, TX, USA), and TRPV4 (
1:200
,
ab39260
;
Abcam
, Cambridge, UK) at 4 °C overnight. After being washed with PBS twice, the sections were incubated
(3)
Mechanoregulatory role of TRPV4 in prenatal skeletal development
Science Advances
January 27, 2023
Nidal S. Khatib, James Monsen, Saima Ahmed, Yuming Huang, David A. Hoey, Niamh C. Nowlan
with 5% normal
goat
serum for 2 hours, incubated with primary antibodies against TRPV4 protein (
1:500
;
ab39260
,
Abcam
) or pHH3 (
1:500
;
ab5176
,
Abcam
) at 4°C overnight, followed by Alexa Fluor 488–conjugated secondary
(4)
Curved Nanofiber Network Induces Cellular Bridge Formation to Promote Stem Cell Mechanotransduction
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
January 1, 2023
Sun Q, Pei F, Zhang M, Zhang B, Jin Y, Zhao Z, Wei Q.
monoclonal anti‐
HDAC1
(D5C6U) (
Cell signaling
,
34589S
,
1:200
dilution), rabbit monoclonal anti‐TRPV4 (
Abcam
,
ab39260
,
1:200
dilution), rabbit monoclonal anti‐Piezo1 (Alpmone labs, APC‐087,
1:200
dilution), rabbit monoclonal
(5)
Blocking TRPV4 Ameliorates Osteoarthritis by Inhibiting M1 Macrophage Polarization via the ROS/NLRP3 Signaling Pathway
Antioxidants (Basel, Switzerland)
November 23, 2022
Sun H, Sun Z, Xu X, Lv Z, Li J, Wu R, Fei Y, Tan G, Liu Z, Liu Y,
et al
.
analyze the protein greyscale values. 2.9. Antibodies The antibodies used were: rabbit anti-TRPV4 (#
ab39260
,
Abcam
, Cambridge, Britain); rabbit anti-iNOS (#
13120
,
Cell Signaling Technology
, Danvers, MA, USA);
(6)
Mechanical Confinement and DDR1 Signaling Synergize to Regulate Collagenâ€Induced Apoptosis in Rhabdomyosarcoma Cells
Advanced Science
October 1, 2022
Jordi Gonzalezâ€Molina, Katharina Miria Kirchhof, Bhavik Rathod, Lidia Moyanoâ€Galceran, Maria Calvoâ€Noriega, Georgia Kokaraki, Astrid Bjørkøy, Monika Ehnman, Joseph W. Carlson, Kaisa Lehti
sc‐47778
;
Santa Cruz Biotechnology
;
1:2000
), MMP14 (
clone
LEM‐2/15.8;
MAB3328
;
Merk
;
1:2000
), and TRPV4 (
ab39260
;
Abcam
;
1:750
). Secondary antibodies
for western blot
used were
goat
anti‐mouse Immunoglobulins/HRP
(7)
Glucosylsphingosine evokes pruritus via activation of 5‐HT
2A
receptor and TRPV4 in sensory neurons
British Journal of Pharmacology
May 1, 2022
Babina Sanjel, Bo‐Hyun Kim, Myung‐Hyun Song, Earl Carstens, Won‐Sik Shim
blocking. The samples were then incubated overnight at 4°C with TRPV4 rabbit polyclonal antibody (
Abcam
,
ab39260
, RRID:AB_1143677) at
1:200
dilution and HTR2A
goat
polyclonal antibody (
Abcam
,
ab140824
) at
1:200
dilution.
(8)
Capsaicin inhibits intestinal Cl- secretion and promotes Na+ absorption by blocking TRPV4 channels in healthy and colitic mice
Journal of Biological Chemistry
May 1, 2022
Hanxing Wan, Xiong Ying Chen, Fenglian Zhang, Jun Chen, Fenglan Chu, Zachary M. Sellers, Feng Xu, Hui Dong
and colonic tissues from C57BL/6 and TRPV4 KO
mice
were incubated with an anti-TRPV4 antibody (
Abcam
,
ab39260
;
1:400
) overnight at 4 °C. Then, the primary antibodies were detected with biotinylated goat anti-rabbit
(9)
Multiubiquitination of TRPV4 reduces channel activity independent of surface localization
The Journal of biological chemistry
April 1, 2022
Aisenberg WH, McCray BA, Sullivan JM, Diehl E, DeVine LR, Alevy J, Bagnell AM, Carr P, Donohue JK, Goretzki B,
et al
.
with
incubation
in Dynabeads Protein G preconjugated with anti-TRPV4 antibody (
Abcam
; catalog no.:
ab39260
). For
Drosophila
, 30 heads were collected from 3-day-old adult
flies
expressing TRPV4 variants selectively
(10)
TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease
Journal of neuroinflammation
January 29, 2022
Liu N, Bai L, Lu Z, Gu R, Zhao D, Yan F, Bai J.
Tween-20
) overnight at 4 °C. The
membranes
were incubated by primary antibodies against TRPV4 (
1:1000
;
ab39260
,
Abcam
), sarco/
endoplasmic reticulum
Ca2+-ATPase 2 (
SERCA2
,
1:2000
;
ab137020
,
Abcam
), glucose-regulated
(11)
Activation of TRPV4 by lactate as a critical mediator of renal fibrosis in spontaneously hypertensive rats after moderate- and high-intensity exercise
Frontiers in physiology
January 1, 2022
Zhao B, Xu Y, Chen Y, Cai Y, Gong Z, Li D, Kuang H, Liu X, Zhou H, Liu G,
et al
.
with normal
goat
serum for 30 min at room temperature, and incubated with the anti-
TRPV4
(
1:500
;
Abcam
,
AB39260
) or anti-TGFβ1 (
1:200
; Santa, SC-130348) antibody, followed by
staining
with the tetramethylrhodamine-conjugated
(12)
Cutaneous Neuroimmune Interactions of TSLP and TRPV4 Play Pivotal Roles in Dry Skin-Induced Pruritus
Frontiers in Immunology
December 2, 2021
Wook-Joo Lee, Won-Sik Shim
The samples were then incubated overnight at 4°C with a TRPV4 rabbit polyclonal antibody (catalog #
ab39260
;
Abcam
, Cambridge, UK) at a dilution of
1:200
. The samples were washed with PBS the following day and
(13)
Regulation of the Membrane Trafficking of the Mechanosensitive Ion Channels TRPV1 and TRPV4 by Zonular Tension, Osmotic Stress and Activators in the Mouse Lens
International journal of molecular sciences
November 23, 2021
Nakazawa Y, Petrova RS, Sugiyama Y, Nagai N, Tamura H, Donaldson PJ.
antibody (amino acid residues 880 to the C-terminus of
mouse
TRPV4 protein) was obtained from
Abcam
(#
ab39260
, Cambridge, MA, USA).
Goat
antirabbit secondary antibody conjugated to Alexa Fluor 488 and the
membrane
(14)
Evaluation of AQP4/TRPV4 Channel Co-expression, Microvessel Density, and its Association with Peritumoral Brain Edema in Intracranial Meningiomas
Journal of Molecular Neuroscience
September 1, 2021
Konstantinos Faropoulos, Afroditi Polia, Chrisi Tsakona, Eleanna Pitaraki, Athanasia Moutafidi, George Gatzounis, Martha Assimakopoulou
were subsequently incubated with primary antibodies anti-TRPV4 rabbit polyclonal antibody of
Abcam
(
ab39260
; Cambridge, UK) (dilution
1:200
),
anti AQP4 (4/18
)
mouse
monoclonal antibody (
sc32739
) of
Santa Cruz
(15)
Activation of TRPV4 channels leads to a consistent tocolytic effect on human myometrial tissues
European Journal of Obstetrics & Gynecology and Reproductive Biology: X
April 1, 2021
Daniela Villegas, Olivier Giard, Karine Brochu-Gaudreau, Éric Rousseau
dilution
1:200
), anti-
TRPV3
(Abcam, Cambridge, UK; cat #
ab231150
; dilution
1:200
), anti-TRPV4 (
Abcam
; cat #
ab39260
dilution
1:200
) or rabbit isotype IgG (Dako; Glostrup Denmark; Cat#X0903) as negative control. Diaminobenzidine
(16)
Neuropathy-causing TRPV4 mutations disrupt TRPV4-RhoA interactions and impair neurite extension
Nature communications
March 4, 2021
McCray BA, Diehl E, Sullivan JM, Aisenberg WH, Zaccor NW, Lau AR, Rich DJ, Goretzki B, Hellmich UA, Lloyd TE,
et al
.
rabbit TRPV4 (WB
1:1000
,
Cosmo Bio USA
,
KAL-KM119
), rabbit anti-TRPV4 (WB
1:1000
, IP 5 μg/ml,
Abcam
,
ab39260
), nonspecific rabbit IgG (IP 5 μg/ml, Cell Signaling Technology, 3900), and anti-
mouse
RhoA
(WB
1:1000
,
(17)
Effect of Alpha-Glucosyl-Hesperidin Consumption on Lens Sclerosis and Presbyopia
Cells
February 12, 2021
Nakazawa Y, Doki Y, Sugiyama Y, Kobayashi R, Nagai N, Morisita N, Endo S, Funakoshi-Tago M, Tamura H.
Dallas, antibody Alomone, Jerusalem, Millipore, Billerica, MA, USA), antibody ACC-030, Israel), or TRPV4 antibody (
1:100
,TRPV1
ab39260
,
Abcam
,(
1:100
, Cambridge, UK) Alomone, antibodiesJerusalem, prepared Israel), or TRPV4 antibody (
1:100
,were ab39260, Cambridge, UK)
(18)
Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior
International Journal of Molecular Sciences
February 5, 2021
Jin Kono, Masakatsu Ueda, Atsushi Sengiku, Sylvia O. Suadicani, Osamu Ogawa, Hiromitsu Negoro
Louis, MO, USA,
1:10000
), Anti-Cx26 (Life Technologies/Invitrogen, Waltham, MA, USA,
1:500
), anti-TRPV4 (
ab39260
,
Abcam
, Cambridge, UK, 2 µg/mL), and anti-
GAPDH
(
2118
,
Cell Signaling Technology
, Danvers, MA, USA,
(19)
Cell Deformation at the Air-Liquid Interface Evokes Intracellular Ca2+ Increase and ATP Release in Cultured Rat Urothelial Cells
Frontiers in Physiology
February 3, 2021
Jiliang Wen, Zhenghao Chen, Mengmeng Zhao, Shulu Zu, Shengtian Zhao, Shaoyong Wang, Xiulin Zhang
5%
goat
serum for 1 h. The primary antibodies used were rabbit polyclonal anti-TRPV4 antibody (
Abcam
ab39260
,
1:50
);
mouse
monoclonal anti-cytokeratin AE1/AE3 antibody (
Abcam
ab86734
,
1:100
); rabbit polyclonal
(20)
The nuclear piston activates mechanosensitive ion channels to generate cell migration paths in confining microenvironments
Science Advances
January 8, 2021
Hong-pyo Lee, Farid Alisafaei, Kolade Adebawale, Julie Chang, Vivek B. Shenoy, Ovijit Chaudhuri
reagents were applied
for immunohistochemistry
: anti–NHE-1 (
1:200
;
ab67314
,
Abcam
), anti-TRPV4 (
1:200
;
ab39260
,
Abcam
), anti–β1 integrin (
1:300
;
ab24693
,
Abcam
), anti-vimentin (
1:200
;
ab92547
,
Abcam
), anti-nesprin-3
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Anti-TRPV4 antibody from Abcam
Supplier Page
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Company Info
Citations
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