Anti-RecA antibody ab63797 from Abcam

Product Specs
ItemAnti-RecA antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab63797
Antibody TypePrimary
HostRabbit
Quantity100 UG
ClonalityPolyclonal
IsotypeIgG
ApplicationsImmunoprecipitation, Western blot
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameRECA
ReactivityEscherichia coli
TargetRecA

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular SalmonellaPLOS BiologyAugust 18, 2023Karin Mitosch, Martin Beyß, Prasad Phapale, Bernhard Drotleff, Katharina Nöh, Theodore Alexandrov, Kiran R. Patil, Athanasios Typas(TBS-T) for 1 h and probed with the following antibodies by incubation at 4°C overnight: RecA (Abcam ab63797), 1:5,000; GAPDH (Cell Signaling #8884), 1:10,000; VDAC1 (Cell Signaling #4866), 1:1,000; histone H3

(2) Exploring the Potential of a Genome-Reduced Escherichia coli Strain for Plasmid DNA ProductionInternational Journal of Molecular SciencesJuly 21, 2023Thi Thuy Nguyen, Le Minh Bui, Ji-Young Byun, Byung-Kwan Cho, Sun Chang Kim(anti-MutS rabbit polyclonal; GeneCheck; #GC-M001 or anti-RecA antibody rabbit polyclonal; Abcam; #ab63797) at a 1:2000 dilution in TBST containing 0.5% BSA at 4 °C for 12 h with gentle shaking. Following three

(3) RecA and SSB genome-wide distribution in ssDNA gaps and ends in Escherichia coliNucleic Acids ResearchJune 23, 2023Phuong Pham, Elizabeth A Wood, Michael M Cox, Myron F Goodmananti-Chicken IgY beads) was purchased from Aves Labs, inc (Davis, CA). Polyclonal rabbit anti-RecA antibody (ab63797) was purchased from Abcam, and Protein G Dynabeads was from ThermoFisher. Monarch PCR & DNA clean-up

(4) Metabolic and Morphotypic Trade-Offs within the Eco-Evolutionary Dynamics of Escherichia coliMicrobiology SpectrumOctober 26, 2022Nikola Zlatkov, Moa Elsa Cecilia NÃ¤sman, Bernt Eric Uhlinab174384). To detect RecA levels, a polyclonal rabbit antibody against full RecA (Abcam; catalog no. ab63797) was used. The RNase E antiserum used in this study was raised in rabbit (81). A representative replica

(5) Single strand gap repair: The presynaptic phase plays a pivotal role in modulating lesion tolerance pathwaysPLOS GeneticsJune 2, 2022Luisa Laureti, Lara Lee, Gaëlle Philippin, Michel Kahi, Vincent Pagèsdone. For RecA immunoblot 200ng of total protein extract was loaded and primary antibodies from Abcam (ab63797) were used at a 1:3000 dilution. For Pol II immunoblot 20 μg of total protein extract was loaded and

(6) Distribution of Holliday junctions and repair forks during Escherichia coli DNA double-strand break repairPLOS GeneticsAugust 25, 2021Tahirah Yasmin, Benura Azeroglu, Charlotte A. Cockram, David R. F. Leach‘input’. Immunoprecipitation was performed overnight at 4°C using 1/100 anti-RecA antibody (Abcam, ab63797). Immunoprecipitated (IP) samples were then incubated with Protein G Dynabeads (Life Technologies) for

(7) Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pilieLifeJuly 7, 2021Rafael Custodio, Rhian M Ford, Cara J Ellison, Guangyu Liu, Gerda Mickute, Christoph M Tang, Rachel M Exley(1:10,000 dilution) and goat anti-rabbit IgG–HRP (1:5000, sc-2004; Santa Cruz). Anti-RecA (1:5000 dilution, ab63797; Abcam) followed by goat anti-rabbit IgG–HRP and detection with ECL detection Reagent (GE Healthcare)

(8) “Gene accordions” cause genotypic and phenotypic heterogeneity in clonal populations of Staphylococcus aureusNature CommunicationsDecember 1, 2020Darya Belikova, Angelika Jochim, Jeffrey Power, Matthew T. G. Holden, Simon HeilbronnerWestern immunoblotting using standard procedures. Filters were incubated with rabbit α-RecA antibody (1:3000) (Abcam, ab63797)18 and goat α-rabbit IgG-DYLight 800 (LI-COR 926-32211) and fluorescence intensity was quantified using

(9) An intrinsic mechanism for coordinated production of the contact-dependent and contact-independent weapon systems in a soil bacteriumPLOS PathogensOctober 9, 2020Mingming Yang, Shuangshuang Ren, Danyu Shen, Nianda Yang, Bingxin Wang, Sen Han, Xi Shen, Shan-Ho Chou, Guoliang Qianand separated by SDS-PAGE. For Western blots, the following antibodies where used: rabbit anti-RecA (ab63797) (Abcam, UK), polyclonal antibody to Clp (Institute of Microbiology, Chinese Academy of Sciences), His

(10) Alkylative damage of mRNA leads to ribosome stalling and rescue by trans translation in bacteriaeLifeSeptember 17, 2020Erica N Thomas, Kyusik Q Kim, Emily P McHugh, Thomas Marcinkiewicz, Hani S Zaher,Santa Cruz Biotechnology,Cat#: sc-53152; RRID:AB_772487,WB: (1:500) Antibody,Rabbit polyclonal anti-RecA,Abcam,Cat#: ab63797; RRID:AB_1142554,WB: (1:10000) Antibody,Rabbit polyclonal anti-m6A,Synaptic Systems,Cat#: 202 003;

(11) Bacterial rhomboid proteases mediate quality control of orphan membrane proteinsThe EMBO JournalMay 18, 2020Guangyu Liu, Stephen E Beaton, Adam G Grieve, Rhiannon Evans, Miranda Rogers, Kvido Strisovsky, Fraser A Armstrong, Matthew Freeman, Rachel M Exley, Christoph M Tanggoat anti‐rabbit IgG‐HRP (Santa Cruz Biotechnology, sc‐2004, 1:5,000). RecA was detected using anti‐RecA antibody (Abcam, ab63797, 1:5,000) and goat anti‐rabbit IgG‐HRP (Santa Cruz Biotechnology, sc‐2004, 1:5,000). Bands were detected

(12) Characterization of a transcriptional TPP riboswitch in the human pathogen Neisseria meningitidisRNA BiologyMay 3, 2020Francesco Righetti, Solange Lise Materne, John Boss, Hannes Eichner, Emmanuelle Charpentier, Edmund Lohand each blot displayed is a representative result. Primary antibodies used were anti-RecA (Abcam, ab63797) and anti-GFP (Clontech Monoclonal Ab JL8, 632381). Secondary antibodies were anti-rabbit IgG-HRP (GE

(13) Contribution of σ ⁷⁰ and σ ^N Factors to Expression of Class II pilE in Neisseria meningitidisJournal of BacteriologyOctober 15, 2019Mariya Lobanovska, Christoph M. Tang, Rachel M. Exley1:10,000) and goat anti-rabbit IgG–HRP (final dilution, 1:10,000; Santa-Cruz). Anti-RecA (1:5,000) (ab63797; Abcam) followed by goat anti-rabbit IgG–HRP (final dilution, 1:10,000; Santa-Cruz) or Coomassie blue

(14) Increasing the bactofection capacity of a mammalian expression vector by removal of the f1 oriCancer Gene TherapyJuly 1, 2019Síle A. Johnson, Michael J. Ormsby, Anne McIntosh, Stephen W. G. Tait, Karen Blyth, Daniel M. Wall(ThermoFisher Scientific) before Western blotting was carried out using antibodies against either RecA (Abcam, ab63797) or GroEL (Abcam, ab90522). Data analysis All values are expressed as the mean±standard error of the

(15) The prokaryotic Argonaute proteins enhance homology sequence-directed recombination in bacteriaNucleic Acids ResearchApril 23, 2019Lei Fu, Caiyun Xie, Zehua Jin, Zizhuo Tu, Li Han, Meilin Jin, Yaozu Xiang, Anding Zhangthen were subjected for immunoblot analysis with rabbit polyclonal antibodies against recA (Abcam, ab63797) or rabbit polyclonal antibodies against Bacteriophage phic31 integrase (Abcam, ab93248). Images were

(16) A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9Nature CommunicationsDecember 1, 2018Lun Cui, Antoine Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikardantibodies to SpCas9 (ab189380, Abcam, diluted 10,000-fold) and rabbit polyclonal antibody to RecA (ab63797, Abcam, diluted 3,000-fold) were used. Goat anti-rabbit horseradish peroxidase (ab6721, Abcam) was used

(17) Hfq, a RNA Chaperone, Contributes to Virulence by Regulating Plant Cell Wall–Degrading Enzyme Production, Type VI Secretion System Expression, Bacterial Competition, and Suppressing Host Defense Response in Pectobacterium carotovorumMolecular Plant-Microbe Interactions®November 1, 2018Chunting Wang, Tianxin Pu, Wangying Lou, Yujie Wang, Zishu Gao, Baishi Hu, Jiaqin Fananti-rabbit lgG-HRP (Abmart), after it had been hybridized with a 1:3,000 dilution of anti-RecA antibody (ab63797) (Abcam, Cambridge, U.K.), which was used as loading control and followed the same steps as above. sRNA

(18) Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA CoproteaseMolecular cellOctober 4, 2018Wipperman MF, Heaton BE, Nautiyal A, Adefisayo O, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies anti-RecA (E. coli),Abcam,Ab63797 Anti-RecA (M. smegmatis),"Pocono Rabbit Farm & Laboratory, Inc.",custom antibodies anti-RpoB,Biolegend,663905 anti-FtsY,"Pocono

(19) RecBCD coordinates repair of two ends at a DNA double-strand break, preventing aberrant chromosome amplificationNucleic Acids ResearchJuly 27, 2018Martin A White, Benura Azeroglu, Manuel A Lopez-Vernaza, A M Mahedi Hasan, David R F Leachpipetting. Immunoprecipitation was performed overnight at 4°C using 1/100 anti-RecA antibody (Abcam, ab63797). Immunoprecipitated samples were then incubated with Protein G Dynabeads® for 2 h with rotation at

(20) Species-specific activity of antibacterial drug combinationsNatureJuly 4, 2018Ana Rita Brochado et al.(A0545 Sigma) at 1:5,000 dilution. Cell loading was controlled with the anti-RecA antibody (rabbit, ab63797 Abcam). For densitometry analysis, pixel intensity of AcrA bands from cell density normalised samples

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Anti-RecA antibody from Abcam

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Anti-RecA antibody from Abcam

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