Anti-Serotonin antibody ab66047 from Abcam

Product Specs
ItemAnti-Serotonin antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab66047
Antibody TypePrimary
Quantity100 UL
HostGoat
ApplicationsImmunohistochemistry (PFA perfusion fixed frozen sections)
ClonalityPolyclonal
IsotypeIgG
Conjugate/TagUnconjugated
TargetSerotonin
ReactivityRat (Rt)

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Modulatory Effects of Noradrenergic and Serotonergic Signaling Pathway on Neurovascular CouplingResearch SquareJuly 13, 2023Robert B Renden, Robert Institoris, Kushal Institoris, Cam Ha T. SharmaInvitrogen). 5-HT neurons were immunostained by incubating with goat anti-5-HT antibody (1:500, Abcam ab66047) and similarly labeled with Alexa 647-conjugated donkey anti-goat secondary antibody (1:1000, Invitrogen). Confocal Microscopy

(2) Long-Term Soft-Food Rearing in Young Mice Alters Brain Function and Mood-Related BehaviorNutrientsMay 20, 2023Furukawa M, Tada H, Raju R, Wang J, Yokoi H, Yamada M, Shikama Y, Matsushita K.ab126751; Abcam), anti-T-cadherin (1:50, #PA5-20158; Invitrogen, Waltham, MA, USA), anti-serotonin (1:400, ab66047; Abcam), anti-5-hydroxy-tryptamine (5-HT) subtype 2A (5-HT2A; 1:300, ab66049; Abcam), anti-5-HT subtype

(3) Baixiangdan capsule and Shuyu capsule regulate anger-out and anger-in, respectively: GB1–mediated GABA can regulate 5-HT levels in multiple brain regionsAging (Albany NY)March 31, 2023Xiaoju Liu, Haijuan Wang, Xiaoyu Wang, Yinxia Ning, Wei Liu, Jie Gaoup, and anti-GB1 antibody (1:1000, Abcam, USA, ab55051) and anti-5-HT antibody (1:1200, Abcam, USA, ab66047) were added to incubate for 1 h at room temperature and then overnight at 4°C. After washing three times

(4) Enteroendocrine cell lineages that differentially control feeding and gut motilityeLifeFebruary 22, 2023Marito Hayashi, Judith A Kaye, Ella R Douglas, Narendra R Joshi, Fiona M Gribble, Frank Reimann, Stephen D Liberlesanti-NTS (Immunostar 20072, 1:2000), rabbit anti-SST (Novus 906552 MAB2358, 1:1000), goat anti-5HT (Abcam ab66047, 1:2000), donkey anti-rabbit Alexa488, Cy3, Cy5, Alexa680 (Jackson Immuno Research, Thermo Fisher, 1:500),

(5) Neurexins in serotonergic neurons regulate neuronal survival, serotonin transmission, and complex mouse behaviorseLifeJanuary 25, 2023Cheung A, Konno K, Imamura Y, Matsui A, Abe M, Sakimura K, Sasaoka T, Uemura T, Watanabe M, Futai K.(guinea pig, 1 µg/ml, Frontier Institute, HTT-GP-Af1400, RRID: AB_2571777), anti-5-HT (goat, 1:1000, Abcam, ab66047, AB_1142794), anti-RIM1/2 (rabbit, 1:1000, Synaptic Systems, Rb 140203, AB_887775), or anti-RFP (Rabbit,

(6) Targeted Methylation Profiling of Single Laser-Capture Microdissected Post-Mortem Brain Cells by Adapted Limiting Dilution Bisulfite Pyrosequencing (LDBSP)International Journal of Molecular SciencesDecember 8, 2022Renzo J. M. Riemens, Gunter Kenis, Jennifer Nolz, Sonia C. Susano Chaves, Diane Duroux, Ehsan Pishva, Diego Mastroeni, Kristel Van Steen, Thomas Haaf, Daniël L. A. van den HovePBS. Sections were then placed in a dilution of the primary antibody against serotonin (5-HT; Abcam, ab66047) in PBS for 10 min at room temperature. After the incubation, sections were washed three times in PBS

(7) Distribution Features and Potential Effects of Serotonin in the Cerebrum of SOD1 G93A Transgenic MiceeneuroOctober 20, 2022Pei He, Binjun He, Shu Li, Wen Chai, Wei Rao, Yu Zhu, Wenzhi Chen, Ping Zhang, Xiong Zhang, Haili Pan, et al.PBS and incubated with the following primary antibodies: goat anti-serotonin (5-HT) antibody 1:200 (ab66047, Abcam Ltd.), rabbit anti-TPH2 antibody 1:400 (ab184505, Abcam Ltd.), rabbit anti-Mash1 antibody 1:100

(8) Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyeliaEBioMedicineMarch 1, 2022Xu T, Li X, Guo Y, Uhlin E, Holmberg L, Mitra S, Winn D, Falk A, Sundström E.(Chemicon, AB1782, 1:1000), S100A10 (Thermo, PA595505, 1:250), ED1 (Bio-Rad, MCA341R, 1:100), 5HT (Abcam, ab66047, 1:500), AQP 4 (Sigma, HPA014784, 1:500),IL1β (R&D systems, AF-501-NA,1:20),TNFα (R&D systems, AF-510-NA,1:20),IL1RA

(9) Cellular and Molecular Gradients in the Ventral Horns With Increasing Distance From the Injury Site After Spinal Cord ContusionFrontiers in Cellular NeuroscienceFebruary 10, 2022Ilyas M. Kabdesh, Yana O. Mukhamedshina, Svetlana S. Arkhipova, Davran K. Sabirov, Maxim S. Kuznecov, Alexandra B. Vyshtakalyuk, Albert A. Rizvanov, Victoria James, Yuri A. Chelyshev(IEM),Sigma–Aldrich (N8912) ALDH1L1,rabbit,1:100 (IEM),Abcam (ab87117) 5-HT,goat,1:400 (IHC),Abcam (ab66047) NeuN,rabbit,1:100 (IHC),Sigma-Aldrich (SAB4300883) Olig2,rabbit,1:75 (IHC),Santa Cruz (sc-48817) Anti-goat

(10) Foxa2 and Pet1 Direct and Indirect Synergy Drive Serotonergic Neuronal DifferentiationFrontiers in neuroscienceJanuary 1, 2022Aydin B, Sierk M, Moreno-Estelles M, Tejavibulya L, Kumar N, Flames N, Mahony S, Mazzoni EO.#R960-25; Tuj1 (MS): Covance #mms-435p; Tuj1 (Rb): Sigma #T2200;5-HT (Rb): Sigma #S5545; 5-HT (Gt): Abcam # Ab66047; TH (Rb): Peel-Freez #P40101-0; TH (Ms): Chemicon #MAB318; TPH1/2 (Sheep): Millipore #AB1541. Alexa

(11) Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammationScience advancesNovember 5, 2021Lee JS, Lee SB, Kim DW, Shin N, Jeong SJ, Yang CH, Son CG.Wako Biologicals), anti-rat C1q monoclonal (1:10; ab11861, Abcam), anti-goat 5-HT polyclonal (1:200; ab66047, Abcam), anti-rabbit TPH2 polyclonal (1:500; NB100-74555, Novus), anti-rabbit TH polyclonal (1:500;

(12) Korean Red Ginseng Ameliorates Fatigue via Modulation of 5-HT and Corticosterone in a Sleep-Deprived Mouse ModelNutrientsSeptember 6, 2021Kang JY, Kim DY, Lee JS, Hwang SJ, Kim GH, Hyun SH, Son CG.buffer (5% normal chicken serum and 0.3% Triton X-100 in ice-cold PBS) and incubated with a 5-HT (1:200; ab66047, Abcam), TPH2 (1:200; NB100-74555, Novus), DA (1:100; NB110-2538, Novus), or TH (1:200; NB300-109, Novus)

(13) STAT3 in the dorsal raphe gates behavioural reactivity and regulates gene networks associated with psychopathologyMolecular PsychiatryJuly 1, 2021Sonali N. Reisinger, Spyros Sideromenos, Orsolya Horvath, Sophia Derdak, Ana Cicvaric, Francisco J. Monje, Martin Bilban, Martin Häring, Micaela Glat, Daniela D. Pollak5-HT/STAT3, Iba1/STAT3, GFAP/STAT3, STAT3 and NeuN stainings (primary antibodies: goat anti-5-HT, Abcam, ab66047, 1:200; rabbit anti-STAT3, Santa Cruz Biotechnology, sc-7179, 1:100; rabbit anti-Iba1, Fujifilm Wako

(14) Optogenetic activation of local colonic sympathetic innervations attenuates colitis by limiting immune cell extravasationImmunityMay 11, 2021Schiller M, Azulay-Debby H, Boshnak N, Elyahu Y, Korin B, Ben-Shaanan TL, Koren T, Krot M, Hakim F, Rolls A.ab18207; RRID: AB_444319 Rat anti-DAT,Abcam,Cat# ab5990; RRID: AB_305226 Goat anti-serotonin,Abcam,Cat# ab66047; RRID: AB_1142794 Rabbit anti-SERT,Millipore,Cat# PC177L; RRID: AB_2122553 Goat anti-CD31,R&D systems,Cat#

(15) Activation of PPG neurons following acute stressors differentially involves hindbrain serotonin in male ratsNeuropharmacologyApril 1, 2021Leon RM, Borner T, Stein LM, Urrutia NA, De Jonghe BC, Schmidt HD, Hayes MR.Peninsula Laboratories), mouse anti-c-Fos (1:500; sc-271243; Santa Cruz), donkey anti-5-HT (1:500; ab66047; ABCAM), and mouse anti-beta subunit Cholera Toxin (1:500; ab62429; ABCAM). Secondary antibodies were

(16) Dietary Lipid Modulation of Intestinal Serotonin in Ballan Wrasse (Labrus bergylta)—In Vitro AnalysesFront Endocrinol (Lausanne)March 23, 2021Angela Etayo, Hoang T. M. D. Le, Pedro Araujo, Kai K. Lie, Øystein Sælestored at -20°C until further use. A commercial monoclonal antibody was used to detect 5-HT in the gut (ab66047, Goat Anti 5-HT antibody, 1:800, Abcam). Sections were left at room temperature (RT) overnight, post-fixed

(17) Altered Visual Function in a Larval Zebrafish Knockout of Neurodevelopmental Risk Gene pdzk1Investigative ophthalmology & visual scienceMarch 1, 2021Xie J, Jusuf PR, Bui BV, Dudczig S, Sztal TE, Goodbourn PT.an NMDA receptor subunit; 114 011, 1:400; Synaptic Systems, Goettingen, Germany), or anti-serotonin (ab66047, 1:500; Abcam) primary antibodies diluted in FBS. Slides were subsequently incubated for 2 hours in

(18) Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal HomeostasisImmunityJanuary 12, 2021Chen Z, Luo J, Li J, Kim G, Stewart A, Urban JF Jr, Huang Y, Chen S, Wu LG, Chesler A, et al.resource,Source,Identifier Antibodies,, Anti-Tublin-βIII,Biolegend,801202 Anti-GFP,Abcam,ab290 Anti-ChgA,Abcam,ab15160 Anti-ST2 for histology,Proteintech,60112–1-Ig Anti-5-HT,Abcam,ab66047 Brilliant Violet 421™ anti-ST2,Biolegend,145309 APC anti-EpCam,Biolegend,118214 Anti-TRPA1,Novus Biologicals

(19) A Comparison of Isolation Stress and Unpredictable Chronic Mild Stress for the Establishment of Mouse Models of Depressive DisorderFrontiers in Behavioral NeuroscienceJanuary 7, 2021Jin-Seok Lee, Ji-Yun Kang, Chang-Gue Sonionized calcium-binding adaptor molecule 1 (Iba-1, 1:200, 019-19741, Wako) or 5-hydroxytryptamine (5-HT, 1:200, ab66047, Abcam) overnight at 4◦ C. Subsequently, the sections were incubated with goat anti-rabbit IgG HRP (1:400,

(20) Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironmentScientific reportsDecember 29, 2020Bellák T, Fekécs Z, Török D, Táncos Z, Nemes C, Tézsla Z, Gál L, Polgári S, Kobolák J, Dinnyés A, et al.18-0063, Thermo Fisher Scientific), rat anti-MOG (1:200, MAB2439, R&D Systems), goat anti-5-HT (1:500, ab66047, Abcam), mouse anti-CS-56 (1:200, C8035, Sigma-Aldrich), biotinylated Griffonia Simplicifolia isolectin

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Anti-Serotonin antibody from Abcam

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Anti-Serotonin antibody from Abcam

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