Anti-CD68 antibody [ED1] ab31630 from Abcam

Product Specs
ItemAnti-CD68 antibody [ED1]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab31630
Antibody TypePrimary
Quantity125 UG
ClonalityMonoclonal
HostMouse, Nude mouse
ApplicationsFlow Cytometry, Immunohistochemistry (Frozen sections)
IsotypeIgG1
CloneED1
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NameCD68
ReactivityRat
TargetCD68

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal DefectsOrganogenesisDecember 31, 2023Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato, et al.CD11b (Biolegend; 1:200; 201807) positive cells were further separated based on CD68 (Abcam; 1:200; ab31630) and CD163 (Abcam; 1:200; ab182422) expression. Animals Peripheral bronchi were harvested from 3-month-old

(2) Gelatin-Based Hydrogel for Three-Dimensional Neuron Culture ApplicationACS OmegaDecember 5, 2023Yu-Yun Gao, You-Ren Ji, Yi-Hua Hsu, Fang-Chun Liao, Nien-Tzu Kao, Abel Po-Hao Huang, Tai-Horng Youngrabbit anti-Iba1 (1:1000; 019-19741; FUJIFILM Wako, Japan) for microglia, and mouse anti-CD68 (1:1000; ab31630; Abcam) for monocytes and macrophages. For signal amplification, the sections were exposed to an avidin–biotin–peroxidase

(3) PRMT5 mediates FoxO1 methylation and subcellular localization to regulate lipophagy in myogenic progenitorsCell ReportsNovember 28, 2023Kun Ho Kim, Stephanie N. Oprescu, Madigan M. Snyder, Aran Kim, Zhihao Jia, Feng Yue, Shihuan KuangPolyclonal anti-SYM10,Millipore,"Cat# 07–412, RRID: AB_310594" Mouse Monoclonal anti-CD68,Abcam,"Cat# Ab31630, RRID: AB_1141557" Mouse Monoclonal anti-Pax7,DSHB,"Cat# Pax7, RRID: AB_2299243" Mouse Monoclonal

(4) Timing of Mesenchymal Stromal Cell Therapy Defines its Immunosuppressive Effects in a Rat Lung Transplantation ModelCell TransplantationNovember 11, 2023Yukinori Tanoue, Tomoshi Tsuchiya, Takuro Miyazaki, Mayumi Iwatake, Hironosuke Watanabe, Hiroshi Yukawa, Kazuhide Sato, Go Hatachi, Koichiro Shimoyama, Keitaro Matsumoto, et al.previously described (see the Supplemental Materials and Methods for details) using mouse anti-CD68 (ab31630, 1:500; Abcam, Cambridge, UK), rabbit anti-CD163 (ab182422, 1:500; Abcam), rabbit anti-CC chemokine

(5) Rab Geranylgeranyltransferase Subunit Beta as a Potential Indicator to Assess the Progression of Amyotrophic Lateral SclerosisBrain SciencesOctober 30, 2023Jing Yang, Cheng Xin, Jia Huo, Xin Li, Hui Dong, Qi Liu, Rui Li, Yaling Liudonkey anti-rat IgG (H+L) highly cross-adsorbed secondary antibody (CD68(1:500, Abcam, Waltham, MA, USA, ab31630), F4/80(1:200, Abcam, Waltham, MA, USA, ab6640), and RABGGTB (1:500, GeneTeX, San-Antonio, TX, USA,

(6) Vascular regeneration and skeletal muscle repair induced by long-term exposure to SDF-1α derived from engineered mesenchymal stem cells after hindlimb ischemiaExperimental & Molecular MedicineOctober 2, 2023Jin-Ju Kim, Jae-Hyun Park, Hyeok Kim, Woo-Sup Sim, Seokbeom Hong, Yeon-Jik Choi, Hyo-Jin Kim, Soon Min Lee, Dongha Kim, Sun-woong Kang, et al.anti-αSMA (Abcam, ab5617; 1:200), rabbit anti-laminin (Sigma, L9393; 1:200), mouse anti-CD68 (Abcam, ab31630; 1:200), rabbit anti-CD206 (Abcam, ab64693; 1:200) and rabbit anti-iNOS (Abcam, ab15323; 1:200). After

(7) Microencapsulated stem cells reduce cartilage damage in a material dependent manner following minimally invasive intra-articular injection in an OA rat modelMaterials Today BioOctober 1, 2023Castro Johnbosco, Lisanne Karbaat, Nicoline M. Korthagen, Kelly Warmink, Michelle Koerselman, Katja Coeleveld, Malin Becker, Bas van Loo, Bram Zoetebier, Sanne Both, et al.blocking with 0.3% H2O2 for 10 min, and 5 w/v % PBS-BSA in PBS for 15 min. CD68 & iNOS antibodies (Abcam ab31630), 1:250 and (Santa Cruz sc7271), 1:100 were incubated for 1 h at room temperature. After washing, the

(8) Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regenerationBioactive materialsOctober 1, 2023Sun Y, Zhang H, Zhang Y, Liu Z, He D, Xu W, Li S, Zhang C, Zhang Z.Abcam, ab10404), anti-β-catenin (1:1000, Cell Signaling Technology, #8480), anti-CD68 (1:100, Abcam, ab31630), anti-Mannose Receptor (1:100, Abcam, ab64693), anti-iNOS (1:70, Abcam, ab15323), and the secondary

(9) Effects of different obesogenic diets on joint integrity, inflammation and intermediate monocyte levels in a rat groove model of osteoarthritisFrontiers in PhysiologyJuly 13, 2023K. Warmink, J. L. Rios, D. R. van Valkengoed, P. Vinod, N. M. Korthagen, H. WeinansPrimary antibody incubation was done overnight at 4°C with 0.75 μg/mL CD68 mouse monoclonal antibody (ab31630, Abcam, Cambridge, United Kingdom) or with 0.75 μg/mL normal mouse IgG1 as negative control (sc3877,

(10) The effect of chronic kidney disease on tissue formation of in situ tissue-engineered vascular graftsAPL BioengineeringJune 1, 2023Paul J. Besseling, Merle M. Krebber, Joost O. Fledderus, Martin Teraa, Krista den Ouden, Melanie van de Kaa, Petra M. de Bree, Aurelie Serrero, Carlijn V. C. Bouten, Patricia Y. W. Dankers, et al.standard techniques. Graft sections were stained (60 min at RT) using CD68 (pan-macrophage marker, ab31630, 1:250; Abcam,) and CD163 (M2 macrophage marker; ab182422, 1:500; Abcam) and visualized with NovaRED

(11) The neuroprotective N-terminal amyloid-β core hexapeptide reverses reactive gliosis and gliotoxicity in Alzheimer’s disease pathology modelsJournal of NeuroinflammationMay 27, 2023Megan J. Lantz, Alyssa M. Roberts, Donovan D. Delgado, Robert A. Nicholsisolated cortical mixed glial cultures (see next section) using anti-rat CD68 (1:500; Abcam catalog # ab31630; RRID:AB_1141557), rabbit anti-TNFα (1:500; Abcam catalog # ab183218; RRID: AB_2889388), or rabbit monoclonal

(12) EphB4 monomer inhibits chronic graft vasculopathy in an aortic transplant modelJVS-Vascular ScienceMay 22, 2023John T. Langford, Luis Gonzalez, Ryosuke Taniguchi, Anand Brahmandam, Weichang Zhang, Alan Dardikserum albumin for 1 hour at room temperature and then incubated overnight at 4°C with either CD68 (ab31630; Abcam) or CD3 (ab11089). Sections were then treated with secondary antibodies at room temperature for

(13) An Atmospheric Plasma Jet Induces Expression of Wound Healing Genes in Progressive Burn Wounds in a Comb Burn Rat Model: A Pilot StudyJournal of burn care & research : official publication of the American Burn AssociationMay 2, 2023Lee Y, Ricky S, Lim TH, Kim H, Lee EJ, Song Y, Lee S, Jang Y.Cell Signaling Technology, Massachusetts), anti-elastase (ab21595, 1:200, rabbit polyclonal, Abcam), and anti-CD68 (ab31630, 1:100, mouse monoclonal, Abcam) primary antibodies in a humidified chamber at 4°C overnight. SignalStain® Boost IHC detection reagents

(14) Epigallocatechin-3-Gallate Attenuates Leukocyte Infiltration in 67-kDa Laminin Receptor-Dependent and -Independent Pathways in the Rat Frontoparietal Cortex following Status EpilepticusAntioxidants (Basel)April 20, 2023Ji-Eun Kim, Duk-Shin Lee, Tae-Cheon KangNumber),Dilution 67LR,IgG,Rabbit,Abcam(ab133645),1:100 CCR2,IgG,Rabbit,Abcam(ab227015),1:100 CD68,IgG,Mouse,Abcam(ab31630),1:100 GFAP,IgG,Mouse,"Millipore, Burlington, MA, USA($MAB3402)",1:4000 IB4,lectin,-,"Vector Laboratories,

(15) 3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve RegenerationAdvanced ScienceApril 1, 2023Yongcong Fang, Chengjin Wang, Zibo Liu, Jeonghoon Ko, Li Chen, Ting Zhang, Zhuo Xiong, Lei Zhang, Wei Sunsections were stained with anti‐CD68 (M0, pan‐macrophage) (1:100, mouse monoclonal antibody, Abcam, ab31630), anti‐iNOS (M1, pro‐inflammatory) (1:70, rabbit polyclonal antibody, Abcam, ab15323), and anti‐Mannose Receptor

(16) Macrophage contribution to the survival of transferred expanded skin flap through angiogenesisAnnals of Translational MedicineMarch 31, 2023Zhaosong Huang, Jianke Ding, Yajuan Song, Wei Liu, Chen Dong, Yu Zhang, Tong Wang, Jing Du, Shaoheng Xiong, Qiang He, et al.4',6-diamidino-2-phenylindole. Primary antibodies against the pan-macrophage marker mouse anti-rat CD68 antibody (1:100; ab31630; Abcam), M1 macrophage marker rabbit anti-rat inducible nitric oxide synthase antibody (iNOS; 1:25;

(17) Targeting CXCL1 chemokine signaling for treating cisplatin ototoxicityFrontiers in ImmunologyMarch 31, 2023Raheem F. H. Al Aameri, Entkhab M. A. Alanisi, Adu Oluwatosin, Dheyaa Al Sallami, Sandeep Sheth, Ian Alberts, Shree Patel, Leonard P. Rybak, Vickram Ramkumarpolyclonal IgG CXCL1 (ab269939), Rabbit monoclonal IBA1 (ab178846) and Mouse monoclonal IgG1 CD68 (ab31630) were purchased from Abcam, Mouse monoclonal IgG2a CD45 (05-1410) and anti-glutamate receptor 2 (GluR2)

(18) Multi-omic analysis of the cardiac cellulome defines a vascular contribution to cardiac diastolic dysfunction in obese female miceBasic research in cardiologyMarch 29, 2023Dona MSI, Hsu I, Meuth AI, Brown SM, Bailey CA, Aragonez CG, Russell JJ, Krstevski C, Aroor AR, Chandrasekar B, et al.activity. Hearts were incubated with antibodies against CD3 (1:100; Abcam #ab5690), CD68 (1:100; Abcam #ab31630), or mast cell tryptase (1:100; Abcam #ab2378). Washed slides were incubated with the appropriate HRP

(19) A stretchable wireless wearable bioelectronic system for multiplexed monitoring and combination treatment of infected chronic woundsScience advancesMarch 24, 2023Shirzaei Sani E, Xu C, Wang C, Song Y, Min J, Tu J, Solomon SA, Li J, Banks JL, Armstrong DG, et al.on cryosections. For IHC staining, two primary antibodies [anti-CD3 [SP7] (ab16669) and anti-CD68 (ab31630), Abcam] and two secondary antibodies [donkey anti-mouse, Alexa Fluor 568– and goat anti-rabbit, Alexa

(20) Immunomodulatory hybrid micro-nanofiber scaffolds enhance vascular regenerationBioactive MaterialsMarch 1, 2023Siyang Liu, Liying Yao, Yumeng Wang, Yi Li, Yanju Jia, Yueyue Yang, Na Li, Yuanjing Hu, Deling Kong, Xianhao Dong, et al.at room temperature, and then incubated with primary antibodies for mouse anti-CD68 (1:100, Abcam, ab31630, USA), and rabbit anti-Mannose Receptor antibodies (1:200, Abcam, ab64693, USA) overnight at 4 °C. Alexa

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3/16/2015Specific marker for macrophages Qing YangOur research investigates inflammation during bladder regeneration. Macrophages are one of the inflammatory cells we study. We were trying to find a specific antibody to detect macrophages on rat ... Read Review

Anti-CD68 antibody [ED1] from Abcam

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Anti-CD68 antibody [ED1] from Abcam

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