Anti-HEF1/NEDD-9 antibody [2G9] ab18056 from Abcam

Product Specs
ItemAnti-HEF1/NEDD-9 antibody [2G9]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab18056
Antibody TypePrimary
IsotypeIgG1
ClonalityMonoclonal
Clone2G9
HostMouse, Nude mouse
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity100 UL
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameNEDD9
ReactivityHuman
TargetHEF1 / NEDD-9

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Histone deacetylase inhibitors promote breast cancer metastasis by elevating NEDD9 expressionSignal transduction and targeted therapyJanuary 6, 2023Hu Z, Wei F, Su Y, Wang Y, Shen Y, Fang Y, Ding J, Chen Y.dehydrated, paraffin-embedded, and deparaffinized before being incubated with NEDD9 antibody (1:200, #ab18056, Abcam), Anti-Histone H3 (acetyl K9) (1:200, #ab10812, Abcam) and Phospho-FAK (Tyr397) (1:200, # 44-624

(2) miR-107 is involved in the regulation of NEDD9-mediated invasion and metastasis in breast cancerBMC CancerDecember 1, 2022Jiamin Zhou, Xianglin Sun, Xinyu Zhang, Huan Yang, Zhenglin Jiang, Qianqian Luo, Yifei Liu, Guohua Wangacetate membrane. After blocking with 5% skim milk, the membrane was incubated with NEDD9 (1:1000, ab18056, Abcam, USA) and β-actin (1:10000, A5316, Sigma, USA) antibodies at 4 °C overnight. The membrane was

(3) NEDD9 Restrains dsDNA Damage Response during Non-Small Cell Lung Cancer (NSCLC) ProgressionCancers (Basel)May 20, 2022Mariya Tikhomirova, Iuliia Topchu, Aleksandra Mazitova, Vitaly Barmin, Ekaterina Ratner, Alexey Sabirov, Zinaida Abramova, Alexander Y. Deneka(Ser216) (#9528), p-H2AX (Ser139) (#3257000) (1:1000; Cell Signaling Technology, Danvers, MA, USA); NEDD9 (ab18056, 1:1000), beta-Actin (ab49900, 1:100,000) (Abcam; Cambridge, MA, USA), Vinculin (700062) (1:1000, Invitrogen,

(4) Susceptibility-Associated Genetic Variation in NEDD9 Contributes to Prostate Cancer Initiation and ProgressionCancer ResearchJuly 15, 2021Dong Han et al.protease inhibitor cocktail (Thermo Fisher Scientific) and anti-ERG (ab110639, Abcam), anti-NEDD9 (ab18056, Abcam), anti-NANOG (3580, Cell Signaling), anti-p-Akt S473 (4060, Cell Signaling), anti-Akt (9272,

(5) Melatonin Inhibits the Progression of Oral Squamous Cell Carcinoma via Inducing miR-25-5p Expression by Directly Targeting NEDD9Frontiers in OncologyDecember 2, 2020Yanling Wang, Bo Tao, Jiaying Li, Xiaoqun Mao, Wei He, Qinbiao Chen120 min. Subsequently, immunoblotting was performed with speciﬁc antibodies against NEDD9 (1:1000, ab18056, Abcam, Cambridge, UK) and b-actin (1:2000, b-actin, Abcam, Cambridge, UK). b-actin was used as an internal

(6) ZMYND10, an epigenetically regulated tumor suppressor, exerts tumor-suppressive functions via miR145-5p/NEDD9 axis in breast cancerClinical epigeneticsDecember 4, 2019Wang Y, Dan L, Li Q, Li L, Zhong L, Shao B, Yu F, He S, Tian S, He J, et al.blocking buffer for 1 h. After treatment, the slides were incubated with anti-HEF1 (NEDD9, 1:150; #ab18056; Abcam, USA) at 4 °C. After 20 h, cells were incubated with Alexa Fluor 555-conjugated goat anti-mouse

(7) Vimentin and Non-Muscle Myosin IIA are Members of the Neural Precursor Cell Expressed Developmentally Down-Regulated 9 (NEDD9) Interactome in Head and Neck Squamous Cell Carcinoma CellsTranslational oncologyJanuary 1, 2019Semelakova M, Grauzam S, Betadthunga P, Tiedeken J, Coaxum S, Neskey DM, Rosenzweig SA.subjected to immunoprecipitation followed by Western blot analysis. Primary antibodies used were: NEDD9 (ab18056, Abcam), Vimentin (RV202: sc-32322 mouse monoclonal antibody, Santa Cruz), Vimentin (D21H3, XP Rabbit mAb

(8) NEDD9 promotes oncogenic signaling, a stem/mesenchymal gene signature, and aggressive ovarian cancer growth in miceOncogeneAugust 1, 2018Gabbasov R, Xiao F, Howe CG, Bickel LE, O'Brien SW, Benrubi D, Do TV, Zhou Y, Nicolas E, Cai KQ, et al.pSTAT3Y705 (cat# 9145S), pan-keratin (cat# 4545) (Cell Signaling Technology; Danvers, MA, USA), NEDD9 (cat# ab18056) (Abcam; Cambridge, MA, USA), SV40-TAg (cat# sc-147), β-actin (cat# sc-8432), E-cadherin (cat# sc-7870),

(9) Zoledronic Acid Suppresses Epithelial-to-Mesenchymal Transition and Invasion via Degradation of Ubiquitinated NEDD9 in PC-3 Prostate Cancer CellsJournal of Cancer Science & TherapyJanuary 1, 2018Tomoaki Tanaka, Kazuya Morimoto, Tatsuya Nakatanithe final concentration was 0.5 μM for all the experiments. Antibodies Mouse monoclonal anti-NEDD9(ab18056), anti-β-actin(ab8226), and rabbit polyclonal anti-Snail+Slug (ab63371) antibodies were obtained from

(10) Progesterone and calcitriol reduce invasive potential of endometrial cancer cells by targeting ARF6, NEDD9 and MT1-MMPOncotargetDecember 26, 2017Waheed S, Dorjbal B, Hamilton CA, Maxwell GL, Rodriguez GC, Syed V.

(11) Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migrationNature communicationsOctober 30, 2017Liu JA, Rao Y, Cheung MPL, Hui MN, Wu MH, Chan LK, Ng IO, Niu B, Cheah KSE, Sharma R, et al.(1:200; Santa Cruz SC-271915 or 1:500; BD Transduction Lab 612020), mouse anti-NEDD9 (1:1000; Abcam ab18056), rabbit anti-GFP (1:1000; Abcam ab6556), mouse anti-FAK (1:500; BD Transduction Lab 610088) and Alexa Fluor 568 Phalloidin

(12) The Prognostic Role of NEDD9 and P38 Protein Expression Levels in Urinary Bladder Transitional Cell CarcinomaJournal of oncologyJanuary 1, 2017Harb OA, Haggag R, Ali MM, El Shorbagy S, Abdelbary AM, Abdelaziz LA, Salim RA, Abdel Wahab KM.binding. Then the slides were incubated overnight with mouse monoclonal anti-NEDD9 antibody (NEDD9 clone ab18056, dilution 1 : 100 Abcam, Cambridge, UK) and rabbit polyclonal anti-p38 protein antibody (clone ab197348)

(13) Tumor-suppressive role of HACE1 in hepatocellular carcinoma and its clinical significanceOncology reportsDecember 1, 2016Gao ZF, Wu YN, Bai ZT, Zhang L, Zhou Q, Li X.three times, the specimens were incubated with rabbit monoclonal antibody against human HACE1 (1:200, ab18056; Abcam) at 4˚C overnight. Following washing with PBS for three times, the tissue sections were incubated

(14) Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvationBiochimica et biophysica actaJune 1, 2016Kim JH, Ki SM, Joung JG, Scott E, Heynen-Genel S, Aza-Blanc P, Kwon CH, Kim J, Gleeson JG, Lee JE.mouse anti-MPS1 (cat. #ab11108, Abcam), mouse anti-PLK1 (cat. #ab17057, Abcam), mouse anti-HEF1 (cat. #ab18056, Abcam), mouse anti-beta-Actin (C4) (cat. #sc-47778, Santa Cruz Biotechnology), and mouse anti-Katanin

(15) High expression of HEF1 predicts a poorer prognosis of hepatocellular carcinoma: A retrospective studyMolecular and clinical oncologyFebruary 1, 2016Li M, Yan S, Cai M, Lu J, Zhang M, Yang P, Luo R.binding. Subsequently, the tissue sections were incubated with rabbit anti‑human anti ‑HEF1 (cat. no. ab18056; 10 µg/ml; Abcam, Burlingame, CA, USA) at 4˚C overnight. Replacement of the primary antibody with phosphate

(16) Expression of NEDD9 in hepatocellular carcinoma and its clinical significanceOncology reportsMay 1, 2015Lu P, Wang ZP, Dang Z, Zheng ZG, Li X, Zhou L, Ding R, Yue SQ, Dou KF.washing, the sections were incubated with a monoclonal antibody against human NEDD9 (1:1,000; Abcam; ab18056) in PBS at 4˚C overnight. Biotinylated goat anti-mouse immunoglobulin G (IgG; 1:400; Abcam; ab128976)

(17) β-catenin inhibitor ICAT modulates the invasive motility of melanoma cellsCancer researchApril 1, 2014Domingues MJ, Rambow F, Job B, Papon L, Liu W, Larue L, Bonaventure J.

(18) The aryl hydrocarbon receptor regulates focal adhesion sites through a non-genomic FAK/Src pathwayOncogeneApril 4, 2013Tomkiewicz C, Herry L, Bui LC, Métayer C, Bourdeloux M, Barouki R, Coumoul X.METHODS Reagents and antibodies Antibodies to integrin beta 1 (ab24693 and ab52971), paxillin (ab32084), HEF1(ab18056), FAK (ab40794) and phospho Tyr397 FAK (ab4803) were from Abcam (Paris, France). Antibodies to E-cadherin

(19) The overexpression of scaffolding protein NEDD9 promotes migration and invasion in cervical cancer via tyrosine phosphorylated FAK and SRCPloS oneJanuary 1, 2013Sima N, Cheng X, Ye F, Ma D, Xie X, Lü W.quantification quantitative analysis. Primary antibodies were specific for following proteins: NEDD9 (ab18056) from Abcam, FAK (3285), pFAK (3283), Src (2109), pSRC (2101), Phospho-Tyrosine (9411), Vimentin (3932)

(20) The metastasis gene NEDD9 product acts through integrin β3 and Src to promote mesenchymal motility and inhibit amoeboid motilityJournal of cell scienceApril 1, 2012Ahn J, Sanz-Moreno V, Marshall CJ.

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Anti-HEF1/NEDD-9 antibody [2G9] from Abcam

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Anti-HEF1/NEDD-9 antibody [2G9] from Abcam

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