Anti-ABCA1 antibody [HJ1] ab66217 from Abcam

Product Specs
ItemAnti-ABCA1 antibody [HJ1]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab66217
Antibody TypePrimary
Quantity10 UG, 100 UG
IsotypeIgG2b
CloneHJ1
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostMouse, Nude mouse
ClonalityMonoclonal
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaNeuroscience
Gene NameABCA1
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetABCA1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organizationBiological ResearchJune 13, 2023Ambroise Wu, Ewa Mazurkiewicz, Piotr Donizy, Krzysztof Kotowski, Małgorzata Pieniazek, Antonina J. Mazur, Aleksander Czogalla, Tomasz TrombikPoland). Immunohistochemical analysis was performed using mouse monoclonal anti-ABCA1 antibody (clone HJ1 (ab66217), dilution 1:200, Abcam) on 4-µm-thick paraffin sections mounted on silanized slides (Agilent DAKO,

(2) MiR-122-5p regulates the mevalonate pathway by targeting p53 in non-small cell lung cancerCell death & diseaseApril 1, 2023Zheng YK, Zhou ZS, Wang GZ, Tu JY, Cheng HB, Ma SZ, Ke C, Wang Y, Jian QP, Shu YH, et al.control, anti-p53 (Proteintech, cat#10442-1-AP, dilution 1/2000 in 5% milk/TBS-T), anti-ABCA1 (Abcam, cat#ab66217, dilution 1/1000 in 5% milk/TBS-T), anti-SREBP2 (Proteintech, cat#28212-1-AP, dilution 1/1000 in 5%

(3) Resveratrol Improves the Progression of Osteoarthritis by Regulating the SIRT1-FoxO1 Pathway-Mediated Cholesterol MetabolismMediators of inflammationJanuary 1, 2023Liang C, Xing H, Wang C, Xu X, Hao Y, Qiu B.,1 : 1000 MMP-13 (54KD),Abcam,Ab39012,1 : 3000 GAPDH (37KD),GOODHERE,AB-P-R 001,1 : 1000 ABCA1 (254KD),Abcam,Ab66217,1 : 1000 APOA1 (31KD),Affinity,DF6264,1 : 1000 LXRα (50KD),Affinity,DF6864,1 : 1000 Antibodies

(4) A reduction of Syndecan-4 in macrophages promotes atherosclerosis by aggravating the proinflammatory capacity of macrophagesJournal of Translational MedicineDecember 1, 2022Jiaxin Hu, Ying Zhang, Liaoping Hu, Haiting Chen, Han Wu, Jianzhou Chen, Jun Xie, Biao Xu, Zhonghai Weiinstructions. The antibodies used in the experiment were as follows: GAPDH (ProMab,20,035); ABCA1 (Abcam,ab66217); ABCG1 (Abcam,ab52617); and p-PKCα (Abcam, ab32502;PKCα (Abcam, ab32376). Enzyme-linked immunosorbent assay

(5) Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticityiScienceMay 20, 2022Lu Q, Wu F, Jiao J, Xue L, Song R, Shi Y, Kong Y, Sun J, Gu N, Han MH, et al.primary mouse ApoA1 antibodies (1:500; Invitrogen, MIA1404), mouse ABCA1 antibodies (1:500, Abcam, ab66217), rabbit NeuN antibodies (1:100, Abcam, ab236870), rabbit iba-1 antibodies (1:500, Wako, 019–19741)

(6) Cholesterol and saturated fatty acids synergistically promote the malignant progression of prostate cancerNeoplasiaFebruary 1, 2022Xiaoying Wang et al.which were blocked in 3% milk for 1 h at RT and blotted with antibody overnight at 4°C: anti- ABCA1 (ab66217, AbCam; 1:1000). Peroxidase-conjugated 2° antibody (ECL) was used at 1:3,000 for 1hr at RT. Statistics:

(7) Qihuang Zhuyu Formula Attenuates Atherosclerosis via Targeting PPARγ to Regulate Cholesterol Efflux and Endothelial Cell InflammationOxidative medicine and cellular longevityJanuary 1, 2022Wang M, Xiang Q, Sun W, Zhang H, Shi R, Guo J, Tong H, Fan M, Ding Y, Shi H, et al.binding strength will be. 2.5. Reagents Antibodies of ATP-binding cassette transporter A1 (ABCA1) (ab66217), liver X receptor alpha (LXRα) (ab41902), and interleukin-1β (IL-1β) (ab234437) were purchased from

(8) A nanodrug system overexpressed circRNA_0001805 alleviates nonalcoholic fatty liver disease via miR-106a-5p/miR-320a and ABCA1/CPT1 axisJournal of NanobiotechnologyDecember 1, 2021Jian Li, Jing Qi, Yishu Tang, Huaizheng Liu, Kefu Zhou, Zheren Dai, Lehong Yuan, Chuanzheng Sun(Abcam, ab124957, 1:1000), p-p65 (ab86299), p65 (ab16502), TNF-α (ab183218), IL-6 (ab259341), ABCA1 (ab66217), CPT1 (ab234111) primary antibodies and horseradish peroxidase (HRP)-labeled secondary antibodies were

(9) Knockdown of mesenchymal stem cell‑derived exosomal LOC100129516 suppresses the symptoms of atherosclerosis via upregulation of the PPARγ/LXRα/ABCA1 signaling pathwayInternational Journal of Molecular MedicineOctober 4, 2021Limin Sun, Xin He, Tao Zhang, Yaling Han, Guizhou Tao1:1,000; cat. no. ab176323; Abcam), anti-phospholipid-transporting ATPase ABCA1 (ABCA1; 1:1,000; cat. no. ab66217; Abcam) and β-actin (1:1,000; cat. no. ab8226; Abcam) at 4°C overnight. Following the primary antibody

(10) HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axisMolecular therapy. Nucleic acidsJune 4, 2021Zhang Z, Wen H, Peng B, Weng J, Zeng F.(12544-1-AP, 1:1,000, Proteintech, USA), NPC1L1 (NB400-128, 1:1,000, Novus Biologicals, UK), ABCA1 (ab66217, 1:1,000, Abcam, USA), ABCG1 (ab52617, 1:1,000, Abcam, UK), ABCG5 (27722-1-AP, 1:1,000, Proteintech,

(11) Septin 4 activates PPARγ/LXRα signaling by upregulating ABCA1 and ABCG1 expression to inhibit the formation of THP‑1 macrophage‑derived foam cellsExperimental and Therapeutic MedicineMay 13, 2021Xiaoying Song, Guoliang Yan, Haihui Wang, Danfei Louwith primary antibodies (All Abcam) against Septin 4 (cat. no. ab166788; 1:2,000), ABCA1 (cat. no. ab66217, 1:1,000), ABCG1 (cat. no. ab52617; 1:5,000), PPARγ (cat. no. ab178860; 1:1,000), LXRα (cat. no. ab176323;

(12) PAK1 Silencing Attenuated Proinflammatory Macrophage Activation and Foam Cell Formation by Increasing PPARγ ExpressionOxidative medicine and cellular longevityJanuary 1, 2021Cheng WL, Zhang Q, Li B, Cao JL, Jiao L, Chao SP, Lu Z, Zhao F.,Proteintech group,Rabbit IL-10,20850-1-AP,Proteintech group,Rabbit CD36,sc-21772,Santa,Mouse SR-A,sc-166139,Santa,Mouse ABCA1,ab66217,Abcam,Mouse ABCG1,ab52617,Abcam,Rabbit PPARγ,sc-7273,Santa,Mouse GAPDH,ab181602,Abcam,Rabbit Antibody

(13) 27-Hydroxycholesterol Promotes the Transfer of Astrocyte-Derived Cholesterol to Neurons in Co-cultured SH-SY5Y Cells and C6 CellsFrontiers in Cell and Developmental BiologyNovember 24, 2020Yushan Wang, Xiaona Zhang, Tao Wang, Wen Liu, Lijing Wang, Ling Hao, Mengwei Ju, Rong XiaoAnn Arbor, MI, United States). Primary antibodies for Western blot included mouse anti-ABCA1 (Abcam, ab66217), rabbit anti-ABCG1 (Abcam, ab52617), mouse anti-Caveolin-1 (Abcam, ab17052), rabbit anti-Apolipoprotein

(14) Ox-LDL Causes Endothelial Cell Injury Through ASK1/NLRP3-Mediated Inflammasome Activation via Endoplasmic Reticulum StressDrug design, development and therapyJanuary 1, 2020Hang L, Peng Y, Xiang R, Li X, Li Z.in this study included ASK1 (Abcam, Cambridge, UK, ab131506), p-AKS1 (Abcam, ab81283), ABCA1 (Abcam, ab66217), ABCG1 (Abcam, ab201776), NLRP3 (Abcam, ab214185), ASC (Abcam, ab175449), caspase 1 (Abcam, ab1872), IL-1β

(15) Polysaccharide IV from Lycium barbarum L. Improves Lipid Profiles of Gestational Diabetes Mellitus of Pregnancy by Upregulating ABCA1 and Downregulating Sterol Regulatory Element-Binding Transcription 1 via miR-33Front Endocrinol (Lausanne)February 23, 2018Shuli Yang, Lihui Si, Limei Fan, Wenwen Jian, Huilin Pei, Ruixin Linwhich was blocked by non-fat milk for 30 min. The membranes were incubated with the antibodies ABCA1 (ab66217) and SREBF1 (ab28481), Beta actin (ab6276, as a loading control), goat polyclonal secondary antibody

(16) Role of Liver X Receptor in AD PathophysiologyPloS oneJanuary 1, 2015Sandoval-Hernández AG, Buitrago L, Moreno H, Cardona-Gómez GP, Arboleda G.

(17) Complete morphologies of basal forebrain cholinergic neurons in the mouseeLifeMay 7, 2014Wu H, Williams J, Nathans J.Richmond, VA); mouse anti-GFAP 1:1000 (MAB360; Millipore); mouse anti-ABCA1 monoclonal antibody HJ1 (ab66217; Abcam, Cambridge, MA); mouse anti-β-Actin antibody AC-15 (A5441; Sigma); and rabbit anti-TH, 1:1000

(18) Obesity and altered glucose metabolism impact HDL composition in CETP transgenic mice: a role for ovarian hormonesJournal of lipid researchMarch 1, 2012Melissa N Martinez, Christopher H Emfinger, Matthew Overton, Salisha Hill, Tara S Ramaswamy, David A Cappel, Ke Wu, Sergio Fazio, W Hayes McDonald, David L Hachey, David L Tabb, John M Stafford

(19) Effects of high-density lipoproteins on pancreatic beta-cell insulin secretionArteriosclerosis, thrombosis, and vascular biologyAugust 1, 2010Michelle A Fryirs, Philip J Barter, Mathiyalagan Appavoo, Bernard E Tuch, Fatiha Tabet, Alison K Heather, Kerry-Anne Rye

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Anti-ABCA1 antibody [HJ1] from Abcam

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Anti-ABCA1 antibody [HJ1] from Abcam

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