Anti-Cytokeratin 3/CK-3 antibody [AE5] ab68260 from Abcam

Product Specs
ItemAnti-Cytokeratin 3/CK-3 antibody [AE5]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab68260
Antibody TypePrimary
ClonalityMonoclonal
IsotypeIgG1
Quantity50 UG
CloneAE5
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameKRT3
ReactivityCow, Human, Mouse, Rabbit
TargetCytokeratin 3 / CK-3

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) New characterization and safety evaluation of human limbal stem cells used in clinical application: fidelity of mitotic process and mitotic spindle morphologiesStem Cell Res TherDecember 13, 2023Marija Zekušić, Marina Bujić Mihica, Marija Skoko, Kruno Vukušić, Patrik Risteski, Jelena Martinčić, Iva M. Tolić, Krešo Bendelja, Snježana Ramić, Tamara Dolenec, et al.p63 (DAK-p63, Dako, Denmark, dilution 1:100), Ki67 (MIB-1, Dako, Denmark, dilution 1:100) and CK3 (ab68260, Abcam, Cambridge, UK, dilution 1:50) for 45 min at room temperature. After repeated washings, a secondary

(2) Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair processSTAR ProtocolsMarch 1, 2023Zitong Yu, Rui Hao, Xi Chen, Lu Ma, Yi Zhang, Hui YangAnti-ZO1 tight junction protein rabbit polyclonal (1:500),Abcam,ab221547 Anti-Cytokeratin 3/CK-3 mouse monoclonal (1:100),Abcam,ab68260 FITC conjugated Goat Anti-Rabbit IgG (H+L) (1:200),Servicebio,GB22303 Cy5 conjugated Goat Anti-Mouse IgG (H+L

(3) Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoidsSTAR protocolsJune 17, 2022Eriksen AZ, Møller R, Makovoz B, tenOever BR, Blenkinsop TA.647",Invitrogen,Cat# A21237;Lot 1597026; RRID: AB_1500743 Anti-Cytokeratin3/CK-3 antibody [AE5],Abcam,Cat# ab68260;Lot GR3329790-1; RRID: AB_1140695 Recombinant Anti-Keratin 12/K12 antibody [EPR17882],Abcam,Cat# ab185627Lot

(4) Electron-Beam Irradiated Recombinant Human Collagen-Phosphorylcholine Corneal Implants Retain Pro-Regeneration CapacityFrontiers in Bioengineering and BiotechnologyJune 13, 2022Fiona C. Simpson et al.for immunohistochemical staining with antibodies against cytokeratin 3 and cytokeratin 12 (2Q1040, ab68260, Abcam, United Kingdom) at a 1/50 dilution. Seven-micron frozen sections irradiated, irradiated, and

(5) Hyaluronic acid hydrogels crosslinked via blue light-induced thiol-ene reaction for the treatment of rat corneal alkali burnRegenerative TherapyJune 1, 2022Sun Kyoung Park, Minji Ha, Eun Jeong Kim, Youngyoon Amy Seo, Hyun Jong Lee, David Myung, Hyun-Seung Kim, Kyung-Sun Nah. The slides were washed and incubated with primary antibodies: mouse anti-CK3 monoclonal antibody (ab68260, Abcam, Cambridge, UK; 1:50 dilution), mouse anti-alpha smooth muscle actin monoclonal antibody (ab7817,

(6) Crosslinker-free collagen gelation for corneal regenerationScientific reportsJune 1, 2022Islam MM, Chivu A, AbuSamra DB, Saha A, Chowdhuri S, Pramanik B, Dohlman CH, Das D, Argüeso P, Rajaiya J, et al.condition. Corneal epithelial cell specific mouse cytokeratin (anti-cytokeratin 3 + 12, clone AE5, ab68260, Abcam) and mouse monoclonal antibody against corneal mucin (anti-MUC16, clone X75; ab10029, Abcam)

(7) A human cornea-on-a-chip for the study of epithelial wound healing by extracellular vesiclesiScienceMay 20, 2022Zitong Yu, Rui Hao, Jing Du, Xiaoliang Wu, Xi Chen, Yi Zhang, Wei Li, Zhongze Gu, Hui Yangpolyclonal,Servicebio,Cat# GB11130; RRID:AB_2910250 Anti-Cytokeratin 3/CK-3 mouse monoclonal,Abcam,Abcam Cat# ab68260; RRID:AB_1140695 Cy3 conjugated Goat Anti-Rabbit IgG (H + L),Servicebio,Cat# GB21303; RRID:AB_2861435 FITC

(8) Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by ThioacetamideTissue engineering and regenerative medicineApril 1, 2022Jiang T, Xia G, Yang B, Zhang HW, Yin YS, Tang CW, Yang JH.fluorescence microscope. The antibodies we used were: cytokeratin (CK), villin, and secondary antibody. (Cat. no. ab68260/ab201989/ab150113; Abcam). Scoring was based on the previously proposed method [30]. Western blot analysis Cells

(9) Clinical, histological and immunohistochemistry characteristics of cornea in the sequelae stage of chronic vernal keratoconjunctivitisIndian Journal of OphthalmologyJanuary 1, 2022Sunita Chaurasia, Vivek Jaffet, Sunita Chaurasia, Saumya Jakati, Ajit Hazari, Virender Sangwan,Santa Cruz Biotechnology ABCG2,1:100,SC18841,Santa Cruz Biotechnology P63α,1:100,CST4892,Cell Signaling Technology CK3,1:100,ab68260,Abcam "Alexa Fluor 488, Anti mouse",1:400,A11001,Invitrogen "Alexa Fluor 594, Anti Rabbit",1:400,A11012,Invitrogen List

(10) In vivo biocompatibility evaluation of in situ-forming polyethylene glycol-collagen hydrogels in corneal defectsScientific ReportsDecember 13, 2021Yoon Hong Chun, Sun-Kyoung Park, Eun Jeong Kim, Hyun Jong Lee, Hyewon Kim, Won-Gun Koh, Gabriella Fernandes Cunha, David Myung, Kyung-Sun Namin. The slides were washed and incubated with primary antibodies: mouse monoclonal antibody against CK3 (ab68260, Abcam, Cambridge, UK) diluted 1:50, mouse monoclonal antibody against α-smooth muscle actin (α-SMA)

(11) Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative OphthalmologyBiomedicinesSeptember 1, 2021Khorolskaya JI, Perepletchikova DA, Kachkin DV, Zhurenkov KE, Alexander-Sinkler EI, Ivanova JS, Mikhailova NA, Blinova MI.Antigen,ICC Dilution,"Manufacturer, Country" CK3/12 (ab68260),1/200,"Abcam, Cambridge, UK" CK5 (VP-C400),1/50,"Vector, Burlingame, CA, USA" CK15 (MA5-15567),1/500,"Thermo Fisher Scientific

(12) Fibrin-Plasma Rich in Growth Factors Membrane for the Treatment of a Rabbit Alkali-Burn LesionInternational Journal of Molecular SciencesMay 25, 2021Ronald M. Sánchez-Ávila, Natalia Vázquez, Manuel Chacón, Mairobi Persinal-Medina, Agustín Brea-Pastor, Silvia Berisa-Prado, Luis Fernández-Vega-Cueto, Eduardo Anitua, Álvaro Meana, Jesús Merayo-LlovesImmunostaining of the corneal sections using antibody against cytokeratin 3 (CK 3) (1:100; Catalog number: ab68260, Abcam, Cambridge, UK) was used to identify differentiated corneal epithelial cells and antibodies against

(13) Core transcription regulatory circuitry orchestrates corneal epithelial homeostasisNature communicationsJanuary 18, 2021Li M, Huang H, Li L, He C, Zhu L, Guo H, Wang L, Liu J, Wu S, Liu J, et al.MA1-06312, 1:1000), anti-Cytokeratin 10 (Invitrogen, MA1-06319, 1:1000), anti-Cytokeratin 3 (Abcam, ab68260, 1:1000), anti-Keratin 12 (Abcam, ab124975, 1:1000), anti-rabbit IgG (Alexa Fluor 488 Conjugate, CST,

(14) Loss of FOXC1 contributes to the corneal epithelial fate switch and pathogenesisSignal transduction and targeted therapyJanuary 8, 2021Li M, Zhu L, Liu J, Huang H, Guo H, Wang L, Li L, Gu S, Tan J, Zhong J, et al.MA1-06319), anti-PAX6 (BioLegend, 901301), anti-Cytokeratin 12 (Abcam,ab124975), anti-Cytokeratin 3 (Abcam, ab68260), and anti-ZNF750 (Sigma, HPA023012). RNA interference and quantitative real-time PCR (qPCR) analysis Two

(15) Growth factor-eluting hydrogels for management of corneal defectsMaterials Science and Engineering: CJanuary 1, 2021Clotilde Jumelle, Ehsan Shirzaei Sani, Yukako Taketani, Ann Yung, Fanny Gantin, Sunil K. Chauhan, Nasim Annabi, Reza Danaincubated with rabbit anti-laminin-5 (1/200) (ab14509, Abcam) and mouse anti-cytokeratin 3 (1/200) (ab68260, Abcam) primary antibodies for 1 h at 37°C. After three rinses with PBS, cross-sections were incubated

(16) Peripheral Blood As a Source of Stem Cells for Regenerative Medicine: Emphasis Towards Corneal Epithelial Reconstruction—An In Vitro StudyTissue Engineering and Regenerative MedicineAugust 1, 2020Balu Venugopal, Sumitha Mohan, T. V. Kumary, P. R. Anil Kumarand blocking, the cells were incubated with primary antibody against Cytokeratin 3/12 (CK3/12, Abcam, ab68260) for 1 h, washed and incubated with the secondary antibody (anti-mouse FITC). The cells were counterstained

(17) Long-Term in vivo Evaluation of Orthotypical and Heterotypical Bioengineered Human CorneasFrontiers in bioengineering and biotechnologyJanuary 1, 2020Garzón I, Chato-Astrain J, González-Gallardo C, Ionescu A, Cardona JC, Mateu M, Carda C, Pérez MDM, Martín-Piedra MÁ, Alaminos M.for immunofluorescence analysis were specific of the cornea epithelium: anti-cytokeratin 3/12 (Abcam ab68260), plakoglobin (Abcam ab12083), crystallins CRY-αA (Santa Cruz sc-28306), CRY-αB (Millipore ABN185),

(18) Differentiation of rat adipose-derived mesenchymal stem cells into corneal-like epithelial cells driven by PAX6Experimental and therapeutic medicineFebruary 1, 2018Sun J, Liu WH, Deng FM, Luo YH, Wen K, Zhang H, Liu HR, Wu J, Su BY, Liu YL.polyclonal antibody (cat no. ab5790), mouse anti-cytokeratin (CK) 3/12 monoclonal antibody (cat no. ab68260) and mouse anti-E-cadherin (cat no. ab76055) were obtained from Abcam (Cambridge, MA, USA). Horseradish

(19) Protection of Corneal Limbus from Riboflavin Prevents Epithelial Stem Cell Loss after Collagen Cross-LinkingJournal of ophthalmologyJanuary 1, 2018Lee HK, Ryu JS, Jeong HJ, Kim MK, Oh JY.immunostaining, goat mAb to rabbit p63 (1 : 100, ab124762, Abcam), and mouse mAb to rabbit CK3/12 (1 : 100, ab68260, Abcam, Cambridge, UK) were used as primary antibodies. Secondary antibodies used were goat anti-rat

(20) 3D map of the human corneal endothelial cellScientific reportsJuly 6, 2016He Z, Forest F, Gain P, Rageade D, Bernard A, Acquart S, Peoc'h M, Defoe DM, Thuret G.

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Anti-Cytokeratin 3/CK-3 antibody [AE5] from Abcam

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Anti-Cytokeratin 3/CK-3 antibody [AE5] from Abcam

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