Anti-C5b-9 + C5b-8 antibody [aE11] ab66768 from Abcam

Product Specs
ItemAnti-C5b-9 + C5b-8 antibody [aE11]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab66768
Antibody TypePrimary
IsotypeIgG2a
Quantity100 UL
ApplicationsImmunohistochemistry (Frozen sections)
ClonalityMonoclonal
CloneaE11
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameC9
ReactivityHuman
TargetC5b-9

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Genome-wide screen in human plasma identifies multifaceted complement evasion of Pseudomonas aeruginosaPLOS PathogensJanuary 25, 2023Manon Janet-Maitre, Stéphane Pont, Frerich M. Masson, Serena Sleiman, Julian Trouillon, Mylène Robert-Genthon, Benoît Gallet, Chantal Dumestre-Perard, Sylvie Elsen, Christine Moriscot, et al.membrane deposition/insertion was detected with mouse monoclonal anti-C5b-9 antibody (8 μg/mL, Abcam #ab66768). Antibody binding was revealed with PE-conjugated goat anti-mouse antibodies (2.5 μg/mL, Abcam #ab97041).

(2) The inflammatory pathology of dysferlinopathy is distinct from calpainopathy, Becker muscular dystrophy, and inflammatory myopathiesActa Neuropathologica CommunicationsDecember 1, 2022Nicole Becker, Steven A. Moore, Karra A. Jonesusing HLA-ABC (MHC class I; Agilent (DAKO); clone W6/32; 1:25) and complement C5b-9 (Abcam; clone aE11; ab66768; 1:50). All H&E and immunostained slides were digitally scanned at 20× magnification using the 3DHistech

(3) Miyoshi Muscular Dystrophy Due to Novel Splice Site Variants in DYSF GeneChild Neurol OpenNovember 16, 2022Grace Bryant, Steven A. Moore, James S. Nix, Grace Rice, Murat Gokden, Aravindhan Veerapandiyanexpression was patchy (HLA-ABC; Agilent (DAKO); clone W6/32), and C5b-9 deposits (Abcam; clone aE11; ab66768) were identified on muscle fiber surfaces by immunofluorescence. Taken together, the neuropathologic

(4) Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophagesFrontiers in ImmunologySeptember 27, 2022Gisela Jimenez-Duran, Joseph Kozole, Rachel Peltier-Heap, Eleanor R. Dickinson, Christopher R. Kwiatkowski, Francesca Zappacosta, Roland S. Annan, Nicholas W. Galwey, Eva-Maria Nichols, Louise K. Modis, et al.Alexa Fluor® 546 conjugate",Thermo-Fisher,A10040 Biotinylated C5b-9,"(Abcam, biotinylated in-house)",ab66768 Ruthenylated anti-C6,"(Quidel, ruthenylated in-house)",A219 "Chemicals, Peptides, and Recombinant

(5) Cd59 and inflammation regulate Schwann cell developmenteLifeJune 24, 2022Ashtyn T Wiltbank, Emma R Steinson, Stacey J Criswell, Melanie Piller, Sarah Kucenasneurofascin 186; also referred to as NF186" Antibody,Anti-C5b-8+C5b-9 (mouse monoclonal),Abcam,Cat# ab66768,"(1:500), C5b-8+C5b-9; also referred to membrane attack complex or MAC" Antibody,Alexa Fluor 488 anti-chicken

(6) The role of antibody responses against glycans in bioprosthetic heart valve calcification and deteriorationNature MedicineFebruary 17, 2022Thomas Senage, Anu Paul, Thierry Le Tourneau, Imen Fellah-Hebia, Marta Vadori, Salam Bashir, Manuel GaliÃ±anes, Tomaso Bottio, Gino Gerosa, Arturo Evangelista, et al.monoclonal antibody against human C5b-9 was obtained from Abcam (clone aE11, 10 μg ml−1; catalog no. AB66768). All antibodies were used at optimized saturating conditions. Avidin/biotin blocking kit was obtained

(7) C5b-9 Membrane Attack Complex Formation and Extracellular Vesicle Shedding in Barrett's Esophagus and Esophageal AdenocarcinomaFrontiers in immunologyJanuary 1, 2022Kolka CM, Webster J, Lepletier A, Winterford C, Brown I, Richards RS, Zelek WM, Cao Y, Khamis R, Shanmugasundaram KB, et al.C9 rabbit polyclonal (Sigma #4503059) diluted 1:1000 and C5b-9 mouse monoclonal clone aE11 (Abcam #ab66768), targeted against a C9 neoepitope, diluted 1:2000 was applied for 30min at room temperature. For visualization

(8) Pfs230 yields higher malaria transmission–blocking vaccine activity than Pfs25 in humans but not miceJournal of Clinical InvestigationApril 1, 2021Sara A. Healy et al.minutes and the pellet was then incubated with 10 μg/mL of the mAb anti-C5b-9 + C5b-8 (Abcam, catalog ab66768) for 2 hours on ice. Cells were washed with PBS, centrifuged at 500g for 5 minutes, and stained with

(9) A human monoclonal antibody blocks malaria transmission and defines a highly conserved neutralizing epitope on gametesNature CommunicationsMarch 19, 2021Camila H. Coelho et al.× g for 5 min, and the pellet then incubated with 10ug/mL of the mAb anti-C5b-9 + C5b-8 (Abcam, ref ab66768) for 2 h on ice. Cells were washed with PBS, centrifuged at 500 × g for 5 min, stained with Hoechst

(10) The conundrum of postpartum thrombotic Microangiopathy: case report and considerations for managementBMC NephrologyDecember 1, 2019Katharina Artinger, Gerald Hackl, Gernot Schilcher, Florian Eisner, Marion J. Pollheimer, Christoph Mache, Eva-Christine Weiss, Kathrin Eller, Philipp Ellercomplement antibody (F0201, DAKO, Glosturp, Denmark) and monoclonal mouse anti-human C5b-9 antibody (ab66768, Abcam, Cambridge, MA, USA), followed by secondary antibody incubation with Rhodamine (TRITC)-conjugated

(11) Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosisEMBO molecular medicineNovember 1, 2018Dembla M, Kesharwani A, Natarajan S, Fecher-Trost C, Fairless R, Williams SK, Flockerzi V, Diem R, Schwarz K, Schmitz F.complex (TCC; Fluiter et al, 2014; Michailidou et al, 2015). This antibody was obtained from Abcam (#ab66768; clone αE11) and used for immunofluorescence microscopy in a 1:50 dilution. Additional primary antibodies

(12) Complement Protein C1q Binds to Hyaluronic Acid in the Malignant Pleural Mesothelioma Microenvironment and Promotes Tumor GrowthFrontiers in immunologyJanuary 1, 2017Agostinis C, Vidergar R, Belmonte B, Mangogna A, Amadio L, Geri P, Borelli V, Zanconati F, Tedesco F, Confalonieri M, et al.

(13) Peptide redesign for inhibition of the complement system: Targeting age-related macular degenerationMolecular visionJanuary 1, 2016Rohith R Mohan, Andrea P Cabrera, Reed E S Harrison, Ronald D Gorham, Lincoln V Johnson, Kaustabh Ghosh, Dimitrios Morikis

(14) Adeno-associated virus mediated delivery of an engineered protein that combines the complement inhibitory properties of CD46, CD55 and CD59The Journal of Gene MedicineNovember 9, 2015Derek Leaderer, Siobhan M. Cashman, Rajendra Kumar-Singhcells, cells were incubated for 2.5 hours at room temperature with mouse anti-human C5b-9 (1:100) (ab66768, Abcam, Cambridge MA) in 0.05% triton containing 6% normal goat serum (NGS). Cy3 conjugated goat anti

(15) Topical Application of PPADS Inhibits Complement Activation and Choroidal Neovascularization in a Model of Age-Related Macular DegenerationPLoS ONEOctober 9, 2013Kerstin Birke, Erion Lipo, Marco T. Birke, Rajendra Kumar-Singhserum, 0.5% Triton X-100, 1x PBS (blocking solution). MAC was labeled with a mouse anti-C5b9 antibody (ab66768, Abcam, Cambridge, MA diluted 1:100 in blocking solution) and detected with a Cy3conjugated goat anti-mouse

(16) Role of antilipopolysaccharide antibodies in serum bactericidal activity against Salmonella enterica serovar Typhimurium in healthy adults and children in the United StatesClinical and vaccine immunology : CVIOctober 1, 2013Estela Trebicka, Susan Jacob, Waheed Pirzai, Bryan P Hurley, Bobby J Cherayil

(17) Full functional activity of SSL7 requires binding of both complement C5 and IgAImmunology and cell biologyAugust 1, 2013Natalie Lorenz, Fiona Clow, Fiona J Radcliff, John D Fraser

(18) Initial pulmonary respiration causes massive diaphragm damage and hyper-CKemia in Duchenne muscular dystrophy dogScientific reportsJanuary 1, 2013Nakamura A, Kobayashi M, Kuraoka M, Yuasa K, Yugeta N, Okada T, Takeda S.St Louis, MO, USA), CD11b (MCA1777S, AbD Serotec, Oxford, UK), CD18 (MCA1780, AbD Serotec), C5b-9 (ab66768, Abcam, Cambridge, UK), cleaved caspase 3 (#9661, Cell Signaling Technology, Beverly, MA, USA), LC3

(19) Genetically-engineered pig-to-baboon liver xenotransplantation: histopathology of xenografts and native organsPloS oneJanuary 1, 2012Burcin Ekser, Edwin Klein, Jing He, Donna B Stolz, Gabriel J Echeverri, Cassandra Long, Chih Che Lin, Mohamed Ezzelarab, Hidetaka Hara, Massimiliano Veroux, David Ayares, David K C Cooper, Bruno Gridelli

(20) Decay Accelerating Factor (CD55)–Mediated Attenuation of Complement: Therapeutic Implications for Age-Related Macular DegenerationInvestigative Opthalmology & Visual ScienceDecember 1, 2010Kelly N. Ma, Siobhan M. Cashman, J. Harry Sweigard, Rajendra Kumar-Singh

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12/2/2014FFPE Immunohistochemistry of C5b-9 Mike CampaActivation of the complement system ultimately leads to the formation of the membrane attack complex, or MAC. The MAC is composed of the complement components C5b, C6, C7, C8, and multiple copies of ... Read Review

Anti-C5b-9 + C5b-8 antibody [aE11] from Abcam

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Anti-C5b-9 + C5b-8 antibody [aE11] from Abcam

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