Fig 1: TIA1 and TIAL1 are required for global expression of its mRNA targets(A) Gene expression changes in double Tia1 Tial1 cKO pro-B cells versus control cells. Differentially expressed (DE) genes (FDR < 0.01) are in blue.(B) Gene set enrichment analysis (GSEA) plots showing decreased expression of genes associated to lymphocyte differentiation and activation.(C) Classification of DE genes in double Tia1 Tial1 cKO pro-B cells based on TIAL1 binding within introns, 3' UTRs or other genomic features (iCLIP crosslink peaks, FDR < 0.05).(D) Changes in expression of direct RNA targets of TIAL1.(E) Percentage of TIAL1-targeted DE genes that are up- or downregulated in double Tia1 Tial1 cKO pro-B cells.(F) Ikzf1 and Ikzf3 mRNA expression in control and double Tia1 Tial1 cKO pro-B cells. mRNAseq data shown as fragments per million (FPM).(G) Genomic annotation of TIAL1 crosslink sites in the last exon of Ikzf1 and Ikzf3. Phylogenetic conservation in Euarchontoglires is shown (21 species, default conservation track settings from UCSC).(H) Ikaros and Aiolos protein expression analyzed by FACS. Right, MFI of Ikaros and Aiolos corrected by the MFI of an isotype antibody control. Data from one of the two independent experiments performed with n = 4 mice/genotype. Two-tailed unpaired t test (**p < 0.01, ***p < 0.001, ****p < 0.0001). Each point is data from one mouse. In (A), (D), and (F) mRNAseq data generated with n = 4 samples/genoptype, FDR and adjusted p values are calculated with DESeq2 using BH correction.See also Figures S2 and S3 and Tables S1, S2, and S4.