alpha Tubulin Antibody MA1-19162 from Thermo Fisher Scientific

Product Specs
Itemalpha Tubulin Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMA1-19162
Brand/TrademarkABR (now Thermo Scientific Pierce Antibodies)
Conjugate/TagUnconjugated
Targetalpha Tubulin
CloneTU-01
Quantity100 µg
HostMouse
FormatPBS with 15mM sodium azide; pH 7.4
IsotypeIgG1
ReactivityHuman, Many, Mouse, Porcine, Rat
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
ClonalityMonoclonal
Concentration1 mg/mL
ImmunogenFraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization
NCBI Full Gene Nametubulin, alpha-like 3, tubulin, alpha 4A, tubulin, alpha 1A, tubulin, alpha 1C, tubulin, alpha 1B, tubulin alpha 3c, tubulin alpha 1b, tubulin alpha 3e, tubulin alpha 1c, tubulin beta 2B class IIb, tubulin, beta 2B class IIB, tubulin, beta 2B class IIb, tubulin alpha 1a, tubulin alpha 4a, tubulin alpha like 3
NCBI Gene Aliases2410129E14Rik, bcm948, K-ALPHA-1, TUBA3, TUBA2, TUBA1, LIS3, TUBA6, RGD1565476, RGD1309427, Tuba2, Tuba1, Tuba6, Tuba4, PMGYSA, brdp, bA408E5.3, bA506K6.1, M[a]6, M[a]4, CDCBM7, Tubb2, Tuba-1, ALS22, B-ALPHA-1, H2-ALPHA
Storage Temperature4° C, do not freeze
PurityProtein A
Antibody TypePrimary
Storage4° C, do not freeze

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Novel Fluorescent Benzimidazole-Hydrazone-Loaded Micellar Carriers for Controlled Release: Impact on Cell Toxicity, Nuclear and Microtubule Alterations in Breast Cancer CellsPharmaceuticsJune 16, 2023Rayna Bryaskova, Nikolai Georgiev, Nikoleta Philipova, Ventsislav Bakov, Kameliya Anichina, Maria Argirova, Sonia Apostolova, Irina Georgieva, Rumiana Tzoneva7.4) with 0.1% Tween 20, and labeled with mouse monoclonal antibody against α-tubulin (1:500, Cat. No. MA1-19162, Thermo Scientific, Rockford, IL, USA) for 1 h at room temperature to analyze microtubule organization

(2) Structural basis of tankyrase activation by polymerizationNatureDecember 1, 2022Pillay N, Mariotti L, Zaleska M, Inian O, Jessop M, Hibbs S, Desfosses A, Hopkins PCR, Templeton CM, Beuron F, et al.(immunoprecipitates: 1:4,000, purified proteins: 1:10,000; MABE1016, Millipore), anti-α-tubulin (1:1,000; MA119162, Thermo Fisher) and anti-β-actin (1:1,000; A00702-40, GenScript). Membranes were then washed in TBST

(3) Synthesis and biological evaluation of imidamide analogs as selective anti-trypanosomal agentsBioorganic & Medicinal ChemistryMay 1, 2022Viharika Bobba, Yaxin Li, Marjia Afrin, Raina Dano, Wenjing Zhang, Bibo Li, Bin Suelectrophoresis. Protein were transferred onto PVDF membranes. Tubulin antibody (Thermo Scientific, MA1–19162) was used in the following western analysis. Supplementary Material

(4) Testicular Melatonin and Its Pathway in Roe Deer Bucks (Capreolus capreolus) during Pre- and Post-Rut Periods: Correlation with Testicular InvolutionAnimals (Basel)June 23, 2021Alberto Elmi, Nadia Govoni, Augusta Zannoni, Martina Bertocchi, Chiara Bernardini, Monica Forni, Domenico Ventrella, Maria Laura Baccithen 5 min in 0.2 M NaOH, and then washed again in water) and re-probed for housekeeping α-tubulin (MA1-19162, Thermo Fisher Scientific). The relative protein contents (MT1 or MT2/α-tubulin) were expressed as arbitrary

(5) Depletion of kinesin motor KIF20A to target cell fate control suppresses medulloblastoma tumour growthCommunications biologyMay 11, 2021Qiu R, Wu J, Gudenas B, Northcott PA, Wechsler-Reya RJ, Lu Q.KIF20A(L-13) (Santa Cruz, sc-104954), KIF20A (OriGene, AP01361PU-N), Alpha-tubulin(TU-01) (ThermoFisher, MA1-19162), Ki67(SP6) (Abcam, Ab16667), Active caspase 3 (BD Biosciences, 559565), and NeuN (Millipore, MAB377).

(6) The Mediator Subunit, Med23 Is Required for Embryonic Survival and Regulation of Canonical WNT Signaling During Cranial Ganglia DevelopmentFrontiers in physiologyJanuary 1, 2020Dash S, Bhatt S, Sandell LL, Seidel CW, Ahn Y, Krumlauf RE, Trainor PA.Med23 at 1:500 (LifeSpan BioSciences, cat# LS-C193128) and a-Tubulin at 1:1000 (Life Technologies, cat# MA1-19162). Whole Mount Immunostaining Embryos were harvested in 1X PBS and fixed in 4%PFA in PBS at 4°C overnight.

(7) Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesisBMC veterinary researchApril 27, 2019Bernardini C, Bertocchi M, Zannoni A, Salaroli R, Tubon I, Dothel G, Fernandez M, Bacci ML, Calzà L, Forni M.were probed for the anti-TLR4 antibody (1:500) and an anti-β-tubulin antibody (1:500 of anti β-tubulin MA1–19162, Thermo Fisher Scientific, Rockford, IL, USA). The membranes were developed using a chemiluminescent

(8) Lead optimization of selective tubulin inhibitors as anti-trypanosomal agentsBioorganic & medicinal chemistryApril 15, 2019Zhao A, Li Y, Orahoske CM, Schnur B, Sabbagh A, Zhang W, Li B, Su B.polyacrylamide gels by electrophoresis. Proteins were transferred onto PVDF membranes. Tubulin antibody (Prod # MA1-19162, Life technology) was used in the following western analysis.

(9) Water/ethanol extract of Cucumis sativus L. fruit attenuates lipopolysaccharide-induced inflammatory response in endothelial cellsBMC complementary and alternative medicineJune 25, 2018Bernardini C, Zannoni A, Bertocchi M, Tubon I, Fernandez M, Forni M.NaOH and then washed again in water) and re-probed for housekeeping α-tubulin (1:500 of anti α-tubulin MA1–19162, Thermo Fisher Scientific, Rockford, IL, USA). The relative protein content (HO-1 or TLR4/α-tubulin)

(10) SETD2 Haploinsufficiency for Microtubule Methylation Is an Early Driver of Genomic Instability in Renal Cell CarcinomaCancer researchJune 15, 2018Chiang YC, Park IY, Terzo EA, Tripathi DN, Mason FM, Fahey CC, Karki M, Shuster CB, Sohn BH, Chowdhury P, et al.for 5 min and were used for western blots using antibodies against α-TubK40me3 (18, 23) or α-tubulin (MA1-19162, Thermo Fisher Scientific, Waltham, MA). The nuclear proteins were extracted in cell lysis buffer (20

(11) Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytesJournal of dermatological scienceMay 1, 2018Mori H, Murakami M, Tsuda T, Kameda K, Utsunomiya R, Masuda K, Shiraishi K, Dai X, Tohyama M, Nakaoka H, et al.

(12) 3D collagen architecture induces a conserved migratory and transcriptional response linked to vasculogenic mimicryNature communicationsNovember 21, 2017Velez DO, Tsui B, Goshia T, Chute CL, Han A, Carter H, Fraley SI.against ITGB1 (#4706 from Cell signaling technology, Danver, MA. 1:10,000 dilution) and α−Tubulin (TU-01 MA1-19162, Thermofisher, Waltham, MA. 1:30,000 dilution). Fluorescence-activated cell sorting Wild type MDA-MB-231 cells

(13) Understanding the importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani and its role in subsistence of the parasiteArchives of biochemistry and biophysicsApril 15, 2016Bhardwaj R, Kumar R, Singh SK, Selvaraj C, Dubey VK.

(14) Sera from early-onset, severely preeclamptic women directly modulate HLA-E expression in the EA.hy296 endothelial cell lineJournal of reproductive immunologyOctober 1, 2014Bueno-Sánchez JC, Peña-Alzate S, Peña RB, Agudelo-Jaramillo B, Cadavid-Jaramillo AP, Chaouat G, Maldonado-Estrada JG.

(15) Proteinase-activated receptor 2 expression in the intestinal tract of the horseResearch in veterinary scienceJune 1, 2014Zannoni A, Bombardi C, Dondi F, Morini M, Forni M, Chiocchetti R, Spadari A, Romagnoli N.

(16) Methoxychlor and triclosan stimulates ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an estrogen receptor-dependent pathwayEnvironmental toxicology and pharmacologyMay 1, 2014Kim JY, Yi BR, Go RE, Hwang KA, Nam KH, Choi KC.

(17) Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potentialCell death and differentiationFebruary 1, 2014De Craene B, Denecker G, Vermassen P, Taminau J, Mauch C, Derore A, Jonkers J, Fuchs E, Berx G.(1/60)37 and mouse anti-alpha-tubulin antibody (1/100) (Pierce, Fisher Scientific, Doornik, Belgium; MA1-19162). Keratinocyte culture. Pups were decapitated at the age of 2 days, and their limbs and tail were removed.

(18) A possible involvement of p62/sequestosome-1 in the process of biliary epithelial autophagy and senescence in primary biliary cirrhosisLiver international : official journal of the International Association for the Study of the LiverMarch 1, 2012Sasaki M, Miyakoshi M, Sato Y, Nakanuma Y.

(19) Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cellsExperimental cell researchOctober 15, 2010Tai CJ, Shen SC, Lee WR, Liao CF, Deng WP, Chiou HY, Hsieh CI, Tung JN, Chen CS, Chiou JF, et al.

(20) Polycomb group protein Bmi1 is overexpressed and essential in anchorage-independent colony formation, cell proliferation and repression of cellular senescence in cholangiocarcinoma: tissue and culture studiesHuman pathologyDecember 1, 2009Sasaki M, Yamaguchi J, Ikeda H, Itatsu K, Nakanuma Y.