Fig 2: Fluorescence-activated cell sorting analysis of MAP-2, vWF, ALP and PPAR?-2 following culture of rat mesenchymal stem cells for 10 days in differentiation medium subsequent to exposure to NG and 72 h and 10 days SMG. The gray area is the isotype control. The results represent three independent experiments. MAP-2, microtubule-associated protein 2; vWF, von Willebrand factor; ALP, alkaline phosphatase; PPAR?2, peroxisome proliferator-activated receptor ?2; NG, normal gravity; SMG, simulated microgravity.

Fig 3: (A) Semi quantitative reverse transcription polymerase chain reaction analysis of (A) MAP-2 following neuronal differentiation, (B) ALP following osteogenic differentiation, (C) vWF following endothelial differentiation and (D) PPAR?2 expression following adipogenic differentiation. Gene expression was normalized to endogenous GAPDH. The results represent three independent experiments. *P<0.05 vs. NG group, #P<0.05 vs. 72 h. MAP-2, microtubule-associated protein 2; ALP, alkaline phosphatase; vWF, von Willebrand factor; PPAR?2, peroxisome proliferator-activated receptor ?2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NG, normal gravity; SMG, simulated microgravity.