Fig 1: Spermatogenesis failed to progress beyond prophase in the 46,X,t(Y;1) carrier. Representative spermatocyte spreads immunostained for pH3 (red), H1T2 (red) and DAPI (blue) show presence of metaphase cells [pH3-positive, (A)] and spermatids [H1T2-positive (C)] in the control, but not in the 46,X,t(Y;1) carrier (B and D), respectively.