Fig 2: Doxycycline (doxy) abrogates the degradative and differentiation potential of hEDS-CM. IF analyses of FN-, COLLI-, and TNs-ECM organization, avß3 integrin and Snail1/Slug transcription factor expression, and a-SMA cytoskeleton assembly in control dermal fibroblasts grown in the presence of a pool of CM, recovered from six 72 h-grown control (C + C-CM) and six hEDS (C + hEDS-CM) cell strains, supplemented with 50 µM of doxy. Images are representative of three independent experiments. Scale bar: 12 µm.

Fig 3: Effects of VSCC-SCs, SCC-SCs and HDFs on the epithelial-mesenchymal transition of HSC-3 cells in bone invasion regions. (A) IHC was used to determine the expression levels of Snail. (B) Semi-quantification of Snail expression levels in the bone invasion regions of different groups of HSC-3 cells. Data are shown as the median and IQR, n=4. Statistical analysis was performed by Kruskal-Wallis test followed by Dunn's test. nsP>0.05, *P<0.05, ***P<0.001, ****P<0.0001 vs. HSC-3 or as indicated. HDFs, human dermal fibroblasts; IHC, immunohistochemistry; SCC-SC, squamous cell carcinoma-associated stromal cells; VSCC-SC, verrucous squamous cell carcinoma-associated stromal cells.

Fig 4: Abundance of SMAD3, SMAD2 and SNAIL within human CKD lesions.(A,B) Representative photomicrographs of human kidney specimens immunolabeled for SMAD3 in collecting ducts in renal medulla (200x, A), SMAD3 in distal tubules in renal cortex (200x, B). (C-H) Representative photomicrographs of interstitial fibrosis and tubular atrophy with global glomerulosclerosis in a patient with end-stage kidney disease stained for PAS (400x, C), SMAD3 in collecting ducts in renal cortex and absence of SMAD3 in atrophic tubuli (400x, D), SMAD2 in atrophic tubules surrounded with interstitial fibrosis (400x, E), SNAIL in atrophic tubules surrounded with interstitial fibrosis (400x, F), in podocytes in patient with nephrotic range proteinuria (400x, G) and in podocytes in a patient with sub-nephrotic proteinuria (400x, H).

Fig 5: Representative photomicrographs of hematoxylin and eosin stain (H&E) and immunohistochemical stain (IHC) for E-cadherin, N-cadherin, Twist and Snail(A) H&E stained benign, borderline and malignant phyllodes tumor specimens (magnification × 200); (B) IHC showing E-cadherin expression in epithelial and stromal compartment (magnification × 400); (C) IHC showing N-cadherin expression in epithelial and stroma compartment (magnification × 400); (D) IHC showing Twist expression in epithelial and stroma component (magnification × 400); (E) IHC showing Snail expression in epithelial and stroma component (magnification × 400).