Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] ab179434 from Abcam

Product Specs
ItemAnti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab179434
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
IsotypeIgG
CloneEPR8590-448
HostRabbit
Antibody TypePrimary
ClonalityMonoclonal
Concentration2.151 mg/ml
NCBI Gene AliasesHMG20
ModificationsLinkage-Specific-K63
Gene NameUBC
ReactivityHuman, Mouse, Rat, Recombinant fragment
TargetUbiquitin

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Website: http://www.abcam.com

(1) The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylationVirusesNovember 24, 2023Congcong Huang, Yiping Yin, Pan Pan, Yanping Huang, Siwei Chen, Junkai Chen, Ju Wang, Guoqing Xu, Xuan Tao, Xiao Xiao, et al.ubiquitination was investigated utilizing the anti-ubiquitin (linkage-specific K63) antibody (Abcam, Cat No. ab179434). For the purpose of identifying SUMOylation, cells were subjected to lysis using RIPA buffer (50 mM

(2) Deubiquitinase OTUD5 promotes hepatitis B virus replication by removing K48-linked ubiquitination of HBV core/precore and upregulates HNF4ɑ expressions by inhibiting the ERK1/2/mitogen-activated protein kinase pathwayCellular and Molecular Life SciencesNovember 1, 2023Bin Lou, Guanghua Ma, Xiaopeng Yu, Feifei Lv, Fanjie Xu, Chengdi Sun, Yu Chen(ab2741), GAPDH (ab181602), Ubiquitin linkage-specific K48 (ab140601), Ubiquitin linkage-specific K63 (ab179434) were obtained from Abcam (Abcam, Cambridge, UK). Quantitative RT-PCR (qRT-PCR) Total RNA was extracted

(3) Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunctionAutophagyJune 3, 2023Yaping Lu, Xiang Chen, Xiuxiu Liu, Yi Shi, Zhaocong Wei, Lili Feng, Quan Jiang, Weifeng Ye, Takuya Sasaki, Kohji Fukunaga, et al.anti-SQSTM1/p62 (1:500; Cell Signaling Technology, 5114), anti-ubiquitin (linkage-specific K63, 1:300; Abcam, ab179434), anti-LGALS3/GAL3 (1:200; Santa Cruz Biotechnology, sc-32,790), anti-LAMP1 (1:300; Abcam, ab24170),

(4) Viral subversion of selective autophagy is critical for biogenesis of virus replication organellesNature communicationsMay 10, 2023Lan Y, van Leur SW, Fernando JA, Wong HH, Kampmann M, Siu L, Zhang J, Li M, Nicholls JM, Sanyal S.(Cell Signalling Technology, MA, USA; 1:1000); Mouse recombinant α-(linkage specific K63)-ubiquitin Ab179434 (Abcam, UK; 1:1000); Mouse recombinant α-(linkage specific-K48) ubiquitin Ab140601 (Abcam, UK; 1:1000);

(5) TRIM21-dependent target protein ubiquitination mediates cell-free Trim-AwayCell reportsFebruary 28, 2023Mevissen TET, Prasad AV, Walter JC.specific polyubiquitin (Cell Signaling Technology, 8081); K63-linkage specific polyubiquitin (Abcam, ab179434). Rabbit polyclonal TRAIP antibodies (referred to as TRAIP-I; kindly provided by Alex Wu) used for Trim-Away

(6) SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli DamageResearch (Washington, D.C.)January 1, 2023Wang F, Wang L, Gan S, Feng S, Ouyang S, Wang X, Yuan S.(Proteintech, 60316-1-lg), FAF2 (Proteintech, 16251-1-AP), ubiquitin (Abcam, Ab7780), K63 ubiquitin (Abcam, Ab179434), and K48 ubiquitin (Abcam, Ab140601). SDS-PAGE and Western blotting Proteins were extracted from

(7) TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 proteinJournal of experimental & clinical cancer research : CRDecember 6, 2022Yang X, Zhang Y, Xue Z, Hu Y, Zhou W, Xue Z, Liu X, Liu G, Li W, Liu X, et al.antibody (Abcam, cat#ab140601), recombinant anti-ubiquitin (linkage-specific K63) antibody (Abcam, cat#ab179434), anti-ubiquitin antibody (Abcam, cat#ab134953), cleaved caspase-3 antibody (CST, cat#9661), cleaved

(8) Bilirubin ameliorates murine atherosclerosis through inhibiting cholesterol synthesis and reshaping the immune systemJournal of Translational MedicineDecember 1, 2022Guanmei Wen, Leyi Yao, Yali Hao, Jinheng Wang, Jinbao Liupolyclonal antibody against HMGCR (ab174830, diluted 1:1000), rabbit polyclonal antibody against ubiquitin (ab179434, diluted 1:5000), and mouse monoclonal antibody against GAPDH (ab8245, diluted 1:1000). All antibodies

(9) The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to bacterial infectionThe EMBO journalDecember 1, 2022Bilkei-Gorzo O, Heunis T, Marín-Rubio JL, Cianfanelli FR, Raymond BBA, Inns J, Fabrikova D, Peltier J, Oakley F, Schmid R, et al.Antibodies purchased from Abcam were the following: Cathepsin D (ab75852), Ubiquitin K63‐linkage specific (ab179434), RNF115 (ab187642) and Histone H3 (ab176842). Total‐ubiquitin antibody was purchased from DAKO (ZO458).

(10) Targeting the Autophagy-Lysosome Pathway in a Pathophysiologically Relevant Murine Model of Reversible Heart FailureJACC. Basic to translational scienceDecember 1, 2022Evans S, Ma X, Wang X, Chen Y, Zhao C, Weinheimer CJ, Kovacs A, Finck B, Diwan A, Mann DL.Cathepsin D (a gift from Stuart Kornfeld, Washington University, St. Louis, Missouri, USA); Ubiquitin (ab179434, Abcam); cytochrome C oxidase-IV (COX-IV, Abcam, ab14744); and translocase of the outer mitochondrial membrane

(11) Tripartite motif-containing protein 46 accelerates influenza A H7N9 virus infection by promoting K48-linked ubiquitination of TBK1Virol JNovember 3, 2022Wei Su, Xian-Tian Lin, Shuai Zhao, Xiao-Qin Zheng, Yu-Qing Zhou, Lan-Lan Xiao, Hui Chen, Zheng-Yu Zhang, Li-Jun Zhang, Xiao-Xin Wurecognizing wild-type (WT) Ubiquitin (ab134953), K48-linked Ubiquitin (ab140601), and K63-linked Ubiquitin (ab179434) (all from Abcam). Statistical analysis Data analysis and processing were carried out using GraphPad

(12) Involvement of adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 in diallyl trisulfide-induced cytotoxicity in hepatocellular carcinoma cellsThe Korean Journal of Physiology & PharmacologyNovember 1, 2022Feng Guan, Youming Ding, Yikang He, Lu Li, Xinyu Yang, Changhua Wang, Mingbai HuUSA); anti-phospho -(Ser/Thr) (ab17464), anti-TRAF6 (ab94720), anti-ubiquitin (linkage-specific K63) (ab179434), and anti-ubiquitin (linkage-specific K48) (ab190061) antibodies, Abcam Inc. (Cambridge, MA, USA).

(13) X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in womenCellOctober 13, 2022Yan Y, Wang X, Chaput D, Shin MK, Koh Y, Gan L, Pieper AA, Woo JA, Kang DE.rabbit monoclonal recombinant anti-ubiquitin (linkage-specific K63) antibody [EPR8590-448] (Abcam, ab179434); rabbit polyclonal anti-ubiquitin antibody (Abcam, ab19247); rabbit polyclonal anti-USP11 antibody

(14) Influenza A virus use of BinCARD1 to facilitate the binding of viral NP to importin α7 is counteracted by TBK1-p62 axis-mediated autophagyCellular & molecular immunologyOctober 1, 2022Wang X, Jiang L, Wang G, Shi W, Hu Y, Wang B, Zeng X, Tian G, Deng G, Shi J, et al.mouse anti-ubiquitin mAb (sc-8017, Santa Cruz), and rabbit anti-ubiquitin (linkage-specific K63) mAb (ab179434, Abcam). The mouse anti-PB2, PB1, PA and NP mAbs were produced as previously described [40]. The rabbit

(15) Inhibition of Neddylation Suppresses Osteoclast Differentiation and Function In Vitro and Alleviates Osteoporosis In VivoBiomedicinesSeptember 21, 2022Wu MH, Hsu WB, Chen MH, Shi CS.antibody (GTX627408) was purchased from GeneTex (Irvine, CA, USA). Antiubiquitin (linkage-specific K63) (ab179434) and Cullin-5 (ab184177) antibodies were purchased from Abcam (Cambridge, MA, USA). 2.2. Cell Cultures The

(16) The speckle-type POZ protein (SPOP) inhibits breast cancer malignancy by destabilizing TWIST1Cell Death DiscoverySeptember 17, 2022Chunli Wei, Yun Liu, Xiaoyan Liu, Jingliang Cheng, Jiewen Fu, Xiuli Xiao, Robb E. Moses, Xiaotao Li, Junjiang Fumutagenesis were constructed in pcDNA5/FRT/TO vector. Anti-Ubiquitin (linkage-specific K63) antibody (Abcam. ab179434), and anti-Ubiquitin (linkage-specific K48) antibody (Abcam. ab140601) were purchased from Abcam. Anti-SPOP

(17) TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-IMolecular Therapy - OncolyticsSeptember 15, 2022Xiaowei Fei et al.anti-ubiquitin (linkage-specific K48) (cat. no. ab140601); and anti-ubiquitin (linkage-specific K63) (cat. no. ab179434) antibodies were purchased from Abcam (Cambridge, UK). CHX was purchased from PlantChemMed (PCM) Biology

(18) The E3 Ligase TRIM4 Facilitates SET Ubiquitin-Mediated Degradation to Enhance ER-α Action in Breast CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2022Han D, Wang L, Long L, Su P, Luo D, Zhang H, Li Z, Chen B, Zhao W, Zhang N, et al.ab171613), anti‐Ubiquitin (K48) (Cat# ab140601), anti‐PP2A (Cat# ab32104), anti‐Ubiquitin (K63) (Cat# ab179434) were from Abcam (MA, USA). Anti‐Flag (Cat# F2555) and anti‐HA (Cat# H6908) were from Sigma‐Aldrich.

(19) Subversion of GBP-mediated host defense by E3 ligases acquired during Yersinia pestis evolutionNature communicationsAugust 4, 2022Cao S, Jiao Y, Jiang W, Wu Y, Qin S, Ren Y, You Y, Tan Y, Guo X, Chen H, et al.MA, USA). Antibodies for Ubiquitin linkage-specific K48 (ab140601), Ubiquitin linkage-specific K63 (ab179434), hGBP1 (ab121039), hGBP2 (ab203238), hGBP3 (ab74061), hGBP4 (ab173697), hGBP5 (ab130569), hGBP6 (ab125704),

(20) The ubiquitin-ligase TRAF6 and TGFβ type I receptor form a complex with Aurora kinase B contributing to mitotic progression and cytokinesis in cancer cellsEBioMedicineAugust 1, 2022Song J, Zhou Y, Yakymovych I, Schmidt A, Li C, Heldin CH, Landström M.antibodies against AURKB (Novus, Cat# NBP2-50039, RRID:AB_2895237), K63-linked polyubiquitin (Abcam Cat# ab179434, RRID:AB_2895239) and TβRI (Santa Cruz Biotechnology Cat# sc-398, RRID: AB_632493) with Duolink Detection

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Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] from Abcam

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Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] from Abcam

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