Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 from Abcam

Product Specs
ItemAnti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab176560
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
IsotypeIgG
CloneEPR13478(B)
HostRabbit
Antibody TypePrimary
NCBI Gene AliasesALS22, H2-ALPHA, TUBA1
ClonalityMonoclonal
Gene NameTUBA4A
ReactivityHuman
Targetalpha Tubulin

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(1) Hydrogel platform facilitating astrocytic differentiation through cell mechanosensing and YAP-mediated transcriptionMaterials Today BioOctober 1, 2023Zhongqian Liu, Shijie Mao, Yubin Hu, Feng Liu, Xiaowei Shaowestern blot were anti-GFAP (130300, Invitrogen), anti-S100β (ab52642, Abcam), and anti-α-Tubulin (ab176560, Abcam). Secondary antibodies were HRP-conjugated anti-rat IgG (31470, Invitrogen) and anti-rabbit IgG

(2) Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6Frontiers in Molecular BiosciencesJuly 20, 2023Ana López-Guajardo, Azeer Zafar, Khairat Al Hennawi, Valentina Rossi, Abdulaziz Alrwaili, Jessica D. Medcalf, Mark Dunning, Niklas Nordgren, Torbjörn Pettersson, Ian D. Estabrook, et al.as follows: anti-mouse-acetylated-α-tubulin (T6793, Sigma Aldrich) (1:2,000), anti-rabbit-α-tubulin (ab176560, Abcam) (1:2,000), anti-mouse-GAPDH (60004, Proteintech) (1:2,000), and anti-rabbit-HDAC6 (NB100-56343,

(3) Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcomaCell death & diseaseJuly 17, 2023Guo W, Wang X, Lu B, Yu J, Xu M, Huang R, Cheng M, Yang M, Zhao W, Zou C.cleaved Capase-3 (9661S, Cell Signaling Technology), SLC7A11(12691S, Cell Signaling Technology), Tubulin (ab176560, Abcam) and 4-Hydroxynonenal (ab46545, Abcam), Caspase-3 (ab184787, Abcam), and corresponding horseradish

(4) The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stabilityJournal of Cell ScienceMay 15, 2023Roshan L. Shrestha, Vinutha Balachandra, Jee Hun Kim, Austin Rossi, Pranathi Vadlamani, Subhash Chandra Sethi, Laurent Ozbun, Shinjen Lin, Ken Chin-Chien Cheng, Raj Chari, et al.anti-CHAF1A (ab126625, Abcam) and anti-RbAp48 (ab1765, Abcam) at 1:500 dilution; mouse anti-α-tubulin (ab176560, Abcam), rabbit anti-DAXX (25C12, Cell Signaling Technology), rabbit anti-cyclin E1 (20808, Cell Signaling Technology

(5) Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancerLife science allianceMay 1, 2023Göbel T, Goebel B, Hyprath M, Lamminger I, Weisser H, Angioni C, Mathes M, Thomas D, Kahnt AS.,Santa Cruz Biotechnology Rictor,sc-271081,Santa Cruz Biotechnology α-Tubulin,sc-5286,Santa Cruz Biotechnology α-Tubulin,ab176560,Abcam Antibodies used for immunoblot analysis.

(6) Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stressCell Host & MicrobeMarch 8, 2023Maria Naama, Shahar Telpaz, Aya Awad, Shira Ben-Simon, Sarina Harshuk-Shabso, Sonia Modilevsky, Elad Rubin, Jasmin Sawaed, Lilach Zelik, Mor Zigdon, et al.overnight with the following primary antibodies: α-GADD153/CHOP (SCBT sc-166682) and α-Tubulin (Abcam ab176560). After washing, membranes were incubated with the species-appropriate HRP-conjugated secondary antibodies.

(7) Poison cassette exon splicing of SRSF6 regulates nuclear speckle dispersal and the response to hypoxiaNucleic acids researchJanuary 25, 2023de Oliveira Freitas Machado C, Schafranek M, Brüggemann M, Hernández Cañás MC, Keller M, Di Liddo A, Brezski A, Blümel N, Arnold B, Bremm A, et al.(NBP2-04144, Novus Biologicals), α-mouse BNIP3 (ab10433, Abcam) and α-rabbit alpha-tubulin antibody (ab176560; Abcam). Image acquisition and quantification were performed using the ImageLab software (Bio-Rad). SRSF6–GFP

(8) AZD6738 Inhibits fibrotic response of conjunctival fibroblasts by regulating checkpoint kinase 1/P53 and PI3K/AKT pathwaysFrontiers in PharmacologySeptember 20, 2022Longxiang Huang, Qin Ye, Chunlin Lan, Xiaohui Wang, Yihua Zhu(AF5718, goat polyclonal antibody, 1 mg/ml; R&D Systems, Minneapolis, MN, United States), and α-Tubulin (ab176560, rabbit monoclonal antibody, 1:1,000; Abcam, Cambridge, MA, United States) overnight at 4 °C. After

(9) Activating transcription factor 4 aggravates angiotensin II-induced cell dysfunction in human vascular aortic smooth muscle cells via transcriptionally activating fibroblast growth factor 21The Korean Journal of Physiology & PharmacologySeptember 1, 2022Ke Tao, Ming Li, Xuefeng Gu, Ming Wang, Tianwei Qian, Lijun Hu, Jiang Li1:1,000), ATF4 (ab184909, 1:1,000), α-SMA (ab124964, 1:10,000), SM22α (ab155272, 1:1,000), Tubulin (ab176560, 1:2,000) (all from Abcam, Cambridge, MA, USA), followed by incubation with the secondary antibody (ab96899;

(10) Butein-instigated miR-186-5p-dependent modulation of TWIST1 affects resistance to cisplatin and bioenergetics of Malignant Pleural Mesothelioma cellsCancer Drug ResistanceJuly 3, 2022Mario Cioce, Daniela Rutigliano, Annamaria Puglielli, Vito Michele Faziomembranes were blocked and then probed with antibodies against TWIST1 (ab50887) and anti-alpha tubulin (ab176560) as a loading control (Abcam, Cambridge, MA, USA). After washing, the blots were incubated with horseradish

(11) HuR Affects the Radiosensitivity of Esophageal Cancer by Regulating the EMT-Related Protein SnailFrontiers in OncologyMay 19, 2022Yan Hu, Qing Li, Ke Yi, Chi Yang, Qingjun Lei, Guanghui Wang, Qianyun Wang, Xiaohui XuInc., Danvers, MA, USA),β-actin (1:2000, GB1201, Servicebio, Woburn, MA, USA) and Tubulin (1:2000, ab176560, Abcam, Cambridge, UK). Goat anti-mouse primary antibodies (1:1000, A0208) or goat anti-rabbit secondary

(12) Rational Development and Characterization of a Ubiquitin Variant with Selectivity for Ubiquitin C-Terminal Hydrolase L3BiomoleculesJanuary 1, 2022Hewitt CS, Das C, Flaherty DP.Cambridge, UK); UCHL3-D25E6 (Cell Signaling Technologies), Ab126621 (Abcam); Alpha Tubulin-Ab7291 (Abcam) or Ab176560 (Abcam). Fluorescent secondary antibodies (Licor IRDye 680RD Goat anti-Rabbit and Licor IRDye 800CW

(13) Inhibitors of class I HDACs and of FLT3 combine synergistically against leukemia cells with mutant FLT3Archives of toxicologyJanuary 1, 2022Wachholz V, Mustafa AM, Zeyn Y, Henninger SJ, Beyer M, Dzulko M, Piée-Staffa A, Brachetti C, Haehnel PS, Sellmer A, et al.Biosciences p-STAT5 (Tyr694),ma5-14973,Thermo Fisher thymidylate synthase,#9045,Cell Signaling α-tubulin,Ab176560,Abcam UBCH8,AP2118a,Abgent Vinculin,sc-7336,Santa Cruz WEE1,sc-5285,Santa Cruz List of antibodies

(14) Angiotensin II type 1a receptor loss ameliorates chronic tubulointerstitial damage after renal ischemia reperfusionScientific ReportsDecember 1, 2021Yoko Fujita et al.detect the expression of α-tubulin on the same membranes, a rabbit monoclonal antibody to α-tubulin (ab176560; 1:4000, Abcam) was used. A horseradish peroxidase-conjugated anti-rabbit antibody (ab97051, Abcam)

(15) Biological Effects of BET Inhibition by OTX015 (MK-8628) and JQ1 in NPM1-Mutated (NPM1c) Acute Myeloid Leukemia (AML)BiomedicinesNovember 17, 2021Djamai H, Berrou J, Dupont M, Coudé MM, Delord M, Clappier E, Marceau-Renaut A, Kaci A, Raffoux E, Itzykson R, et al.(#H00004869-M01, CliniSciences, Nanterre, France), anti-BRD4 (#ab128874, Abcam, Paris, France), anti-tubulin (#ab176560, Abcam, Paris, France), or anti-lamin (#ab16048, Abcam, Paris, France). Secondary antibodies were goat

(16) Transforming growth factor-β1 decreases erythropoietin production through repressing hypoxia-inducible factor 2α in erythropoietin-producing cellsJournal of biomedical scienceNovember 2, 2021Shih HM, Pan SY, Wu CJ, Chou YH, Chen CY, Chang FC, Chen YT, Chiang WC, Tsai HC, Chen YM, et al.Santa Cruz Biotechnology, Santa Cruz, CA), total Smad2/3 (3102; Cell Signaling Technology, Danvers, MA), α-tubulin (ab176560; Abcam, Cambridge, UK), β-actin (4967; Cell Signaling), GAPDH (Ab9485; Abcam) and proliferating cell

(17) Prochlorperazine Withdraws the Delayed Onset Tonic Activity of Unloaded Rat Soleus Muscle: A Pilot StudyLife (Basel, Switzerland)October 30, 2021Kalashnikov VE, Tyganov SA, Turtikova OV, Kalashnikova EP, Glazova MV, Mirzoev TM, Shenkman BS.PhosphoSolutions, Aurora, CO, USA), NKCC (1:1000, registry id ab_528406; DSHB, Iowa City, IA, USA), tubulin (cat #ab176560; Abcam, Boston, MA, USA). After that, the membranes were washed in PBST (3 times for 5 min) and incubated

(18) DHHC9-mediated GLUT1 S-palmitoylation promotes glioblastoma glycolysis and tumorigenesisNature communicationsOctober 7, 2021Zhang Z, Li X, Yang F, Chen C, Liu P, Ren Y, Sun P, Wang Z, You Y, Zeng YX, et al.patients. Methods Materials Rabbit monoclonal antibodies recognizing GLUT1 (#ab115730), tubulin (#ab176560), Ki67 (#ab92742) and ATP1A1 (#ab76020), and mouse monoclonal antibody recognizing GAPDH (#ab8245) were

(19) HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophagesJournal of nanobiotechnologySeptember 22, 2021Niu Y, Stadler FJ, Yang X, Deng F, Liu G, Xia H.anti-arginase-1 (1:1000, PA5-85267, Gibco), anti iNOS (1:1000, PA3-030a, Gibco), and anti-α-tubulin (1:1000, ab176560, Abcam), the subsequent procedures were performed as described previously [26–28]. Cytokine secretion To

(20) Receptor-Mediated Mitophagy Rescues Cancer Cells under Hypoxic ConditionsCancers (Basel)August 10, 2021Alibek Abdrakhmanov, Maria A. Yapryntseva, Vitaliy O. Kaminskyy, Boris Zhivotovsky, Vladimir GogvadzeBNIP3 (D7U1T) (all from Cell Signaling Technology), anti-rabbit lc3b (ab51520), anti-mouse α-tubulin (ab176560), anti-rabbit β-actin (ab227387), anti-rabbit BNIP3L (ab8399) (all from Abcam). 4.3. Western Blotting After

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Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control from Abcam

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Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control from Abcam

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