Fig 1: miR-124-3p is expressed at low levels in IS, while CTDSP1, which is a downstream target gene of miR-124-3p, is highly expressed in IS. A miR-124-3p expression in patients with IS compared to that in the control group according to the GEO database. Data are shown as mean ± SD (n = 3). B, C The expression of CTDSP1 in patients with IS compared to the control group according to the GEO database. Data are shown as mean ± SD. D miR-124-3p expression in OGD-exposed neurons. E, F The expression level of CTDSP1 under OGD conditions was verified at the protein and mRNA levels. G Dual-luciferase reporter assay results. H Predicted sites at which miR-124-3p binds to the 3'-UTR of CTDSP1. I, J Representative blots and western blotting analysis of the CTDSP1 levels in the different groups. ß-actin was used as a loading control. A–G and J, mean ± SD (B and C, n > 3; others n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. the respective control. RT?qPCR reverse transcription-quantitative polymerase chain reaction, WT wild-type, MUT mutant-type, UTR untranslated region, mimic miR-124-3p mimic, mimic NC miR-124-3p mimic negative control, inhibitor miR-124-3p inhibitor, inhibitor NC miR-124-3p inhibitor negative control
Fig 2: miR-124-3p alleviates OGD-induced apoptosis and axon damage in neurons through the CTDSP1/AKT signaling pathway. Representative immunoblots and quantitative data are provided. A CTDSP1 expression in different experimental groups. B The p-AKT/t-AKT ratio in different experimental groups. C The Bcl-2/Bax ratio and Cleaved Caspase3 expression in different experimental groups D GAP43 expression in different experimental groups. A–D The data are expressed as the mean ± SD (n = 4). *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. the respective control. mimic miR-124-3p mimic, mimic NC miR-124-3p mimic negative control, oe overexpression of CTDSP1, oe NC overexpression of CTDSP1 negative control, p-AKT phosphorylated AKT, t-AKT total AKT
Fig 3: The ELA/APJ axis regulates miR-124-3p and the downstream signaling pathway. A Quantification of miR-124-3p expression levels in different experimental groups. The data are shown as mean ± SD (n = 3). B, C Representative blots and quantitative analysis of CTDSP1 expression. ß-actin was used as a loading control. The data are presented as mean ± SD (n = 3). D, E Representative blots and quantitative analysis of the p-AKT/t-AKT ratio. The data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ****P < 0.0001 vs. the respective control. ELA Elabela, OGD oxygen–glucose deprivation, siAPJ small interfering apelin receptor, CTDSP1 C-terminal domain small phosphatase 1, p-AKT phosphorylated AKT, t-AKT total AKT
Fig 4: ELA regulates the CTDSP1/AKT pathway via miR-124-3p. A, B Representative blots and quantitative analysis of CTDSP1 expression. ß-actin was used as a loading control. C, D Representative blots and quantitative analysis of p-AKT and t-AKT expression. ß-actin was used as a loading control. E, F Representative blots and quantitative analysis of Bcl-2, Bax, and Cleaved Caspase3 expression; ß-actin was used as a loading control. G, H Representative blots and quantitative analysis of GAP43 expression. ß-actin was used as a loading control. B, D, F and H, the data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the respective control. ELA Elabela, OGD oxygen–glucose deprivation, siAPJ small interfering apelin receptor, CTDSP1 C-terminal domain small phosphatase 1, p-AKT phosphorylated AKT, t-AKT total AKT, inhibitor miR-124-3p inhibitor, inhibitor NC miR-124-3p inhibitor negative control
Supplier Page from Proteintech Group Inc for CTDSP1 antibody