Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody TA50011-100 from OriGene Technologies

Product Specs
ItemClone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
CompanyOriGene Technologies
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberTA50011-100
Quantity100 µl
ApplicationsFC, IF, IHC, IP, WB
ModificationsDDK (FLAG tag): DYKDDDDK
CloneOTI4C5 (formerly 4C5)
HostMouse
ClonalityMonoclonal
Concentration1mg/ml
IsotypeIgG2a
Antibody TypePrimary
ImmunogenAnti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
Conjugate/TagUnconjugated

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OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

(1) SARS-CoV-2 ORF3c impairs mitochondrial respiratory metabolism, oxidative stress, and autophagic fluxiScienceJuly 21, 2023Mozzi A, Oldani M, Forcella ME, Vantaggiato C, Cappelletti G, Pontremoli C, Valenti F, Forni D, Saresella M, Biasin M, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Mouse monoclonal anti-DDK – Clone 4C5,OriGene,"Cat# TA50011-100, RRID:AB_2622345" Rabbit polyclonal anti-DDK antibody,OriGene,Cat# TA100023RRID:AB_2622243 Mouse monoclonal

(2) A functional mechanism for a non-coding variant near AGTR2 associated with risk for preterm birthBMC MedicineJuly 17, 2023Li Wang, Robert M. Rossi, Xiaoting Chen, Jing Chen, Jilian Runyon, Mehak Chawla, Daniel Miller, Carmy Forney, Arthur Lynch, Xuzhe Zhang, et al.protein/binding mix and incubated for 20 min at room temp. For supershift assays, anti-DDK antibody (Origine #TA50011-100) was incubated with the nuclear lysate/binding buffer for 20 min prior to the addition and incubation

(3) Deficiency of the Tmem232 Gene Causes Male Infertility with Morphological Abnormalities of the Sperm Flagellum in MiceCellsJune 13, 2023Xiuqing He, Wenyu Mu, Ziqi Wang, Ke Xu, Yingying Yin, Gang Lu, Wai-Yee Chan, Hongbin Liu, Yue Lv, Shangming LiuLouis, MI, USA) were used for the immunofluorescence assays. Mouse anti-FLAG antibody (1:1000 dilution, TA50011-100, Origene, Rockville, MD, USA) and rabbit anti-HA antibody (1:1000 dilution, 3724S, Cell signaling technology,

(4) Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδNature CommunicationsDecember 1, 2022Yi Liu et al.isolated and characterized for DDK-tagged PPARδ expression by Western blot using anti-DDK antibody (#TA50011-100, 1: 2000, OriGene) and then expanded for further analyses. Generation of Ppard genetic knockout in

(5) Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinolCommunications BiologyDecember 1, 2022Yosuke Iizumi, Yoshihiro Sowa, Wakana Goi, Yuichi Aono, Motoki Watanabe, Yoichi Kurumida, Tomoshi Kameda, Kenichi Akaji, Masatoshi Kitagawa, Toshiyuki Sakai1:500 dilution), uS4 (ab74711, Abcam, 1:1000 dilution), uL3 (ab241412, Abcam, 1:500 dilution), and DDK (TA50011-100, OriGene Technologies, Rockville, MD, USA, 1:500 dilution). The membranes were then incubated with HRP-linked

(6) Exploring the expression patterns of palmitoylating and de-palmitoylating enzymes in the mouse brain using the curated RNA-seq database BrainPalmSeqeLifeJuly 12, 2022Angela R Wild, Peter W Hogg, Stephane Flibotte, Glory G Nasseri, Rocio B Hollman, Danya Abazari, Kurt Haas, Shernaz X BamjiCell Signaling Technology, C29F4, Rabbit mAb CAT#: 3724, 1:1000; anti-FLAG: Origene, mouse monoclonal antibody, CAT#: TA50011-100, 1:1000) overnight at 4 °C. Proteins were then visualized using enhanced chemiluminescence (Immubilon

(7) Development of iPSC-based clinical trial selection platform for patients with ultrarare diseasesScience AdvancesApril 8, 2022Glen Lester Sequiera et al.pECHS1 protein was confirmed by immunoblotting against its DDK tag using anti-DDK antibody (OriGene, no. TA50011100). Cell proliferation assay The proliferation of fibroblasts was assessed using a WST1 assay kit (BioVision

(8) Cannabidiol-induced activation of the metallothionein pathway impedes anticancer effects of disulfiram and its metabolite CuETMolecular oncologyApril 1, 2022Buchtova T, Skrott Z, Chroma K, Rehulka J, Dzubak P, Hajduch M, Lukac D, Arampatzis S, Bartek J, Mistrik M.anti‐β‐actin (1 : 1000; Santa Cruz Biotechnology, cat. no: sc‐47778), anti‐DDK (1 : 1000; OriGene, cat. no: TA50011‐100), anti‐GAPDH (clone 1D4, 1 : 500; GeneTex, cat. no: GTX78213), antihistone H3 (1 : 2000; Cell Signaling,

(9) Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cellsEngineering in Life SciencesFebruary 1, 2022Benno Verbelen et al.cells were fixed in ice‐cold 100% ethanol and stained with 0.1 μg/mL mouse anti‐DDK IgG2a (OriGene, TA50011‐100) followed by 0.1 μg/mL anti‐mouse PE‐conjugated IgG (Cell Signaling Technology, 8887). Cells were analyzed

(10) Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver diseaseNature communicationsJanuary 28, 2022Barbier-Torres L, Murray B, Yang JW, Wang J, Matsuda M, Robinson A, Binek A, Fan W, Fernández-Ramos D, Lopitz-Otsoa F, et al.diluted 1:1000 in 5% skim milk against MATα1 (ab129176), PIN1 (07-091, Millipore), TOM20 (ab78547), DDK (TA50011-100, Origene), His-Tag (A00186-100, GenScript), OXPHOS (ab110413), CK2α (2656, Cell Signaling), CPT1α (12252,

(11) Functional New Transcription Factors (TFs) Associated with Cervical CancerJournal of Healthcare EngineeringJanuary 25, 2022Yicheng Jiang, Shi Chen3.8.2). 2.8. Cell Transfection and Western Blot Analysis Antibodies against β-actin (#TA811000S) and DDK (#TA50011-100) were purchased from OriGene (Beijing, China). Lipofectamine2000 (#11668027) was a product of Thermofish.

(12) An active vesicle priming machinery suppresses axon regeneration upon adult CNS injuryNeuronJanuary 5, 2022Hilton BJ, Husch A, Schaffran B, Lin TC, Burnside ER, Dupraz S, Schelski M, Kim J, Müller JA, Schoch S, et al.Synaptic Systems), Rabbit anti-RIM2 (1:200; 140 103, Synaptic Systems) and/or mouse anti-DDK (FLAG) (1:500, TA50011-100, OriGene) in PBS containing 10% blocking solution at room temperature for 1 h. Cells were then washed

(13) SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposureFrontiers in immunologyJanuary 1, 2022Gandhirajan A, Roychowdhury S, Kibler C, Cross E, Abraham S, Bellar A, Nagy LE, Scheraga RG, Vachharajani V.(tGFP) antibody (Origene, Rockville, MD, CAT# TA150041), DDK antibody (Origene, Rockville, MD, CAT# TA50011-100), Anti-Ubiquitin antibody (EMD Millipore, Burlington, Massachusetts, USA, CAT#5-944), Biotinylated anti-SIRT2

(14) α-TubK40me3 is required for neuronal polarization and migration by promoting microtubule formationNature CommunicationsDecember 1, 2021Xuan Xie, Shaogang Wang, Mingyi Li, Lei Diao, Xingyu Pan, Jijun Chen, Weiguo Zou, Xu Zhang, Wenfeng Feng, Lan Baohomemade), GFP (1:2000; Abcam ab13970), SMI-312 (1:500, Biolegend 837904) or Flag (1:1000; Origene TA50011-100) overnight at 4 °C. The samples were then incubated with corresponding Alexa-Fluor-conjugated secondary

(15) TRAIL sensitivity of nasopharyngeal cancer cells involves redox dependent upregulation of TMTC2 and its interaction with membrane caspase-3Redox biologyNovember 20, 2021Raman D, Tay P, Hirpara JL, Liu D, Pervaiz S.,Santa Cruz Biotechnologies,Western Blotting DR5,FAB6311P,R&D Systems,Flow Cytometry/Confocal analysis DDK,TA50011-100,Origene,Western Blotting Mn SOD,611580,BD Biosciences,Western Blotting TMTC2,ARP49065_P050,Aviva System

(16) Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-altered prostate cancersNature communicationsAugust 20, 2021Mao N, Zhang Z, Lee YS, Choi D, Rivera AA, Li D, Lee C, Haywood S, Chen X, Chang Q, et al.(Cell Signaling Technology, 4875S, 1:10,000), Cyclophilin B (Cell Signaling Technology, 43603S, 1:10,000), DDK tag (Origene, TA50011-100). All western blot antibodies were used at a 1:1000 dilution unless specificied. CRISPR/Cas9-mediated

(17) Use of dual-electron probes reveals the role of ferritin as an iron depot in ex vivo erythropoiesisiScienceAugust 20, 2021Aronova MA, Noh SJ, Zhang G, Byrnes C, Meier ER, Kim YC, Leapman RD.antibody,Abcam,ab69090; RRID:AB_1523609 Anti-Rab11 antibody,Millipore,Cat # 05-853 Anti-DDK antibody,Origene,Cat # TA50011-100; RRID:AB_2622345 Alexa Fluor™ 594 labeled Transferin,Thermo Fisher Scientific,Cat #T13343 ,, Biological

(18) Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysisCell reports. MedicineAugust 17, 2021Hamel Y, Mauvais FX, Madrange M, Renard P, Lebreton C, Nemazanyy I, Pellé O, Goudin N, Tang X, Rodero MP, et al.,Santa Cruz Biotechnology,"Cat# sc-6487, RRID: AB_2292414" Mouse monoclonal anti-DDK (clone OTI4C5),OriGene,"Cat# TA50011-100, RRID:AB_2622345" Goat polyclonal anti-EEA1,Santa Cruz Biotechnology,"Cat# sc-6415, RRID: AB_2096822" Mouse

(19) EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescenceeLifeAugust 3, 2021Gautheron J, Morisseau C, Chung WK, Zammouri J, Auclair M, Baujat G, Capel E, Moulin C, Wang Y, Yang J, et al.(1:1000) Antibody,Anti-FAS,Cell Signaling Technology,Cat# 3180,WB (1:1000) Antibody,Anti-Flag,Origene,Cat# TA50011-100,WB (1:1000) - IF (1:1000) - IP (1:200) Antibody,Anti-IRΒ,Cell Signaling Technology,Cat# 3025,WB (1:1000) Antibody,Anti-IRS1,Protein

(20) Deubiquitinase USP39 and E3 ligase TRIM26 balance the level of ZEB1 ubiquitination and thereby determine the progression of hepatocellular carcinomaCell death and differentiationAugust 1, 2021Li X, Yuan J, Song C, Lei Y, Xu J, Zhang G, Wang W, Song G.

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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody from OriGene Technologies

OriGene Technologies

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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody from OriGene Technologies

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