Fig 2: Expression of IL-19 and its receptors in three vital organs of ischemic AKI mice.(A) AKI mice (n = 5 in each group) were killed 48 h after renal IRI. Paraffined sections of kidney, liver, and lung tissue were stained using anti-IL-19 mAb, anti-IL-20R1 mAb, and anti-IL-20R2 polyclonal Ab. Anti-mIgG1 was a negative control. The reaction was detected using AEC chromogen stain (red), and the nuclei were counterstained with hematoxylin (blue). The bars represent 50 µm. All five mice in each group showed similar patterns. Shown sections are representative of five individual mice. (B) Mouse tissues of each group (n = 5) were isolated and homogenized to extract total protein. Mouse IL-19 expression in kidney, liver, and lung were analyzed using direct ELISA. *P<0.05 compared with sham-operated mice. (C–E) Experimental mice were killed 2 days after renal IRI. RTQ-PCR was done with primers specific for IL-19, IL-20R1, and IL-20R2. The relative quantification of PCR products was expressed as 2-??Ct, corrected using GAPDH expression, and relative to levels of untreated cells. Data are the means ± SD of three experiments. *P<0.05 compared with sham-operated mice.