Fig 2: Phosphorylated OCT4 Prefers to Interact with SOX2 while Non-phosphorylated OCT4 Selectively Binds to SOX17(A) Representative immunoblotting of anti-OCT4 antibody-immunoprecipitated proteins from wild-type, T343A, or T343D ESCs using antibodies to OCT4, SOX2, SOX17, and KLF4.(B) Quantitative measurement of the immunoblotting signal in T343A (Figure 4A, middle lanes) or T343D (Figure 4A, right lanes) ESCs, normalized by each respective signal in wild-type ESCs (Figure 4A, left lanes). Values represent means ± SD from three independent experiments (*p < 0.01; ANOVA).(C) Luciferase-reporter assay for the Nanog promoter. Values represent mean ± SD from three independent replicates (*p < 0.01; ANOVA).(D) Luciferase-reporter assay for the Pdgfra promoter. Values represent mean ± SD from three independent replicates (*p < 0.01; ANOVA).(E) ChIP analysis of OCT4 for the promoter of Oct4, Nanog, and Pdgfra.(F) Relative binding measurement of OCT4 to the promoter of Oct4, Nanog, and Pdgfra. Values represent means ± SD from three independent experiments (*p < 0.01; ANOVA).