Western blot was performed using Anti-EZH1 Polyclonal Antibody (Product # PA1-41114) and a ~90 kDa band corresponding to Histone-lysine N-methyltransferase EZH1 was observed along with few uncharacterised band (*) in the range of 80 kDa to 20 kDa across cell lines tested. Nuclear enriched extracts (30 µg lysate) of MOLT-4 (Lane 1), HCT 116 (Lane 2), TNFa treated HCT 116 (Lane 3), THP-1 (Lane 4), HEL 92.1.7 (Lane 5) and K-562 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).