APC anti-human CD4 300537 from BioLegend

Product Specs
ItemAPC anti-human CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number300537
Quantity500 tests
ApplicationsFlow Cytometry (FC)
Conjugate/TagAPC
Formatµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ReactivityHuman
IsotypeIgG1, Kappa
CloneRPA-T4
HostMouse
Antibody TypePrimary
NCBI Full Gene NameCD4 molecule
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid LeukemiaCancer discoveryFebruary 6, 2023Zhou F, Aroua N, Liu Y, Rohde C, Cheng J, Wirth AK, Fijalkowska D, Göllner S, Lotze M, Yun H, et al.anti-human CD38 antibody (cat. 303506, BioLegend); and a lineage cocktail consisting of CD4-APC (cat. 300514, BioLegend), APC anti-human CD20 (cat. 302310, BioLegend), APC anti-human CD8a (cat. 301014, BioLegend),

(2) Effects of Sirolimus treatment on patients with β‐Thalassemia : Lymphocyte immunophenotype and biological activity of memory CD4 + and CD8 + T cellsJournal of Cellular and Molecular MedicineFebruary 1, 2023Matteo Zurlo, Francesco Nicoli, Davide Proietto, Beatrice Dallan, Cristina Zuccato, Lucia Carmela Cosenza, Jessica Gasparello, Chiara Papi, Elisabetta d'Aversa, Monica Borgatti, et al.Thermo Fisher CD3,PerCP,300428,BioLegend CD14,PE‐Cy7,25‐0149‐42,Invitrogen by Thermo Fisher CD4,APC,300514,BioLegend CD8,APC‐Cy7,557834,BD Pharmingen List of antibodies employed for Lymphocyte immunophenotyping

(3) The Regulation between CD4+CXCR5+ Follicular Helper T (Tfh) Cells and CD19+CD24hiCD38hi Regulatory B (Breg) Cells in Gastric CancerJournal of Immunology ResearchOctober 26, 2022Ying Zhang, Jun Wu, Haibo Zhang, Chen Wubeing washed twice. 2.3. Flow Cytometry Human PBMCs were stained with anti-CD4-APC (BioLegend, USA, 300514), anti-CXCR5-PE/cyanine7 (BioLegend, USA, 356924), anti-CD19-FITC (BioLegend, USA, 392508), anti-CD24-APC

(4) Innate type 2 immunity controls hair follicle commensalism by Demodex mitesImmunityOctober 11, 2022Ricardo-Gonzalez RR, Kotas ME, O'Leary CE, Singh K, Damsky W, Liao C, Arouge E, Tenvooren I, Marquez DM, Schroeder AW, et al."Gata-3 Monoclonal Antibody (TWAJ), eFluor 660, eBioscience™, 100 tests",Invitrogen,Cat#50-9966-42; RRID:AB_10596663 APC anti-human CD4 Antibody,BioLegend,Cat#300514; RRID:AB_314082 "FOXP3 Monoclonal Antibody (FJK-16s), eBioscience™",Invitrogen,Cat#11-5773-82; RRID:AB_465243 "BV605 Rat Anti-Mouse IL-33R (ST2

(5) Hypoxia induces HIF1α-dependent epigenetic vulnerability in triple negative breast cancer to confer immune effector dysfunction and resistance to anti-PD-1 immunotherapyNature communicationsJuly 15, 2022Ma S, Zhao Y, Lee WC, Ong LT, Lee PL, Jiang Z, Oguz G, Niu Z, Liu M, Goh JY, et al.BV711 antibody (Biolegend, CAT# 318336, dilution 1:100), anti-human CD4 APC antibody (Biolegend, CAT# 300514, dilution 1:100), anti-human IFNγ BV785 (Biolegend, CAT# 502542, dilution 1:100), anti-human TNFα BV650

(6) Expression of γ-globin genes in β-thalassemia patients treated with sirolimus: results from a pilot clinical trial (Sirthalaclin)Therapeutic Advances in HematologyJune 21, 2022Cristina Zuccato et al.Waltham, MA, USA" CD3,PerCP,300428,BioLegend CD14,PE-Cy7,25-0149-42,"Invitrogen, Thermo Fisher" CD4,APC,300514,BioLegend CD8,APC-Cy7,557834,"BD PharMingen, Franklin Lakes, NJ, USA" Antibodies used for immunophenotype

(7) Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver diseaseEMBO reportsJune 7, 2022Ng CY, Lee KL, Muthiah MD, Wu KX, Chioh FWJ, Tan K, Soon GST, Shabbir A, Loo WM, Low ZS, et al.antibodies directed against CD8‐PE (clone SK1, Biolegend 344706), CD4‐APC (clone RPA‐T4, Biolegend 300537). Details on antibodies are included in Appendix Table S5. Following 30 min of antibody incubation at

(8) Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapyNature communicationsApril 12, 2022Kim ST, Chu Y, Misoi M, Suarez-Almazor ME, Tayar JH, Lu H, Buni M, Kramer J, Rodriguez E, Hussain Z, et al.Anti-Human CD4 PE-Cy7 (Biolegend, Cat No: 300512; Dilution 1:50), Anti-Human CD4 APC (Biolegend, Cat No: 300514; Dilution 1:50), Anti-Human IL-10 APC (Biolegend, Cat No: 506806; Dilution 1:40), Anti-Human IL-2 APC

(9) Suppression of human and simian immunodeficiency virus replication with the CCR5-specific antibody Leronlimab in two speciesPLOS PathogensMarch 31, 2022Xiao L. Chang et al.Pacific Blue; cat# 558124); b) from BioLegend, OKT4 (CD4; APC-Cy7; cat# 305612), RPA-T4 (CD4; APC; cat# 300537); c) from Beckman Coulter, RMO52 (CD14; PE-Texas Red; cat# IM2707U); d) from Sigma, HP-6025 (IgG4; FITC;

(10) γ9δ2 T-Cell Expansion and Phenotypic Profile Are Reflected in the CDR3δ Repertoire of Healthy AdultsFrontiers in immunologyJanuary 1, 2022Vyborova A, Janssen A, Gatti L, Karaiskaki F, Yonika A, van Dooremalen S, Sanders J, Beringer DX, Straetemans T, Sebestyen Z, et al.(CD8a-PerCP-Cy5.5 Clone RPA-T8, BioLegend; cat. no. 301031), CD4 (CD4-APC Clone RPA-T4, BioLegend; cat. no. 300514), CD27 (CD27-APC-eF780 clone O323, eBioscience, San Diego, CA, USA; cat. no. 47-0279-42) and isotype

(11) Antibody-based CCR5 blockade protects Macaques from mucosal SHIV transmissionNature CommunicationsDecember 1, 2021Xiao L. Chang et al.558124); (b) from BioLegend, OKT4 (CD4; APC-Cy7; 1:100; cat# 305612), RPA-T4 (CD4; APC; 1:100; cat# 300537); (c) from Beckman Coulter, RMO52 (CD14; PE-Texas Red; 1:40; cat# IM2707U), KC57 (HIV Gag p24; FITC;

(12) Modulating tumor infiltrating myeloid cells to enhance bispecific antibody-driven T cell infiltration and anti-tumor responseJournal of Hematology & OncologyDecember 1, 2021Jeong A. Park, Linlin Wang, Nai-Kong V. CheungCD45-Brilliant Violet 421™ (BioLegend Cat# 304032, RRID:AB_2561357), anti-human CD4-APC (BioLegend Cat# 300514, RRID:AB_314082), anti-human CD4-PE/Cyanine7 (BioLegend Cat# 300512, RRID:AB_314080), anti-human CD8-FITC

(13) Applicability of Small-Molecule Inhibitors in the Study of Peptidyl Arginine Deiminase 2 (PAD2) and PAD4Frontiers in ImmunologyOctober 19, 2021María Teresa Martín Monreal, Alexandra Stripp Rebak, Laura Massarenti, Santanu Mondal, Ladislav Šenolt, Niels Ødum, Michael L. Nielsen, Paul R. Thompson, Claus H. Nielsen, Dres DamgaardUSA), 7-AAD (cat. 420403, BioLegend), anti-CD14 PerCP (cat. 325632, BioLegend), anti-CD4 APC (cat. 300514; BioLegend), anti-CD16 Alexa Fluor 700 (cat. 560713, BD Biosciences), anti-CD8 Brilliant Violet 421

(14) NPM–ALK-Induced Reprogramming of Mature TCR-Stimulated T Cells Results in Dedifferentiation and Malignant TransformationCancer ResearchJune 15, 2021Jan M. Pawlicki et al.Untransduced and NPM-ALKKD transduced T cells were purified similarly using anti-CD4-APC (Biolegend, #300537) instead of anti-PD-L1-APC. RNA was extracted using the Qiagen RNeasy Kit (Qiagen, 74104) according

(15) Interrogating the small intestine tuft cell – ILC2 circuit using in vivo manipulationsCurrent ProtocolsMarch 26, 2021Claire E. O'Leary, Xiaogang Feng, Victor S. Cortez, Richard M. Locksley, Christoph SchneiderBiolegend 406421) Anti-Thy1.2 BV785 (Biolegend 105331) Anti-human CD4 (PE Biolegend 300508; APC Biolegend 300514) Anti-IL-17RB APC (Biolegend 146308) Anti-KLRG1 eFluor710 (ThermoFisher 46-5893-82) Anti-Gata3 PE (ThermoFisher

(16) Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer CellsBiology (Basel)February 10, 2021Daria S. Chulpanova, Zarema E. Gilazieva, Sevindzh K. Kletukhina, Aleksandr M. Aimaletdinov, Ekaterina E. Garanina, Victoria James, Albert A. Rizvanov, Valeriya V. SolovyevaPE anti-human CD196 (CCR6) antibody (#353410, BioLegend, San Diego, CA, USA), APC anti-human CD4 antibody (#300514, BioLegend, San Diego, CA, USA), FITC anti-human CD3 antibody (#317306, BioLegend, San Diego, CA, USA),

(17) A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-ReperfusionInternational journal of molecular sciencesFebruary 9, 2021Eleftheriadis T, Pissas G, Crespo M, Nikolaou E, Liakopoulos V, Stefanidis I.the University Thessaly, FacultySan of Jose,study CA, USA) and theThe APC Mouse anti-human CD4 (cat. no. of 300514, Biolegend, medicine (Larissa, Greece) approved the study protocol (Number of approval:558, date: Diego,

(18) Visualizing Cellular Dynamics and Protein Localization in 3D CollagenSTAR protocolsDecember 18, 2020Koh WH, Zayats R, Lopez P, Murooka TT.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Human CD3 (clone: HIT3a),Biolegend,Cat #300318 Human CD4 (clone: RPA-T4),Biolegend,Cat #300537 Human CD11c (clone: 3.9),Biolegend,Cat #337218 Human CD14 (clone: 63D3),Biolegend,Cat #367116 Human

(19) TZM-gfp cells: a tractable fluorescent tool for analysis of rare and early HIV-1 infectionScientific reportsNovember 16, 2020Gludish DW, Boliar S, Caldwell S, Tembo DL, Chimbayo ET, Jambo KC, Mwandumba HC, Russell DG.

(20) Single-cell sequencing reveals clonal expansions of pro-inflammatory synovial CD8 T cells expressing tissue-homing receptors in psoriatic arthritisNature communicationsSeptember 21, 2020Penkava F, Velasco-Herrera MDC, Young MD, Yager N, Nwosu LN, Pratt AG, Lara AL, Guzzo C, Maroof A, Mamanova L, et al.Biolegend: CD3-FITC (SK7, catalogue number 344803, dilution 1:50), CD4-APC (RPA-T4, catalogue number 300514, dilution 1:50), CD8a-PE (RPA-T8, catalogue number 301007, dilution 1:50) and CD45RA-BV421 (HI100, catalogue

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8/14/2019BioLegend CD4 Antibody Works Great! Dechao CaoGood product, easy to analyze!

APC anti-human CD4 from BioLegend

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APC anti-human CD4 from BioLegend

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