APC anti-mouse CD21/CD35 (CR2/CR1) 123412 from BioLegend

Product Specs
ItemAPC anti-mouse CD21/CD35 (CR2/CR1)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number123412
NCBI Gene AliasesCr-1, Cr-2, Cr1, mCRY, C3DR, CD21, CD35, Crry, Mcp
HostRat
Quantity100 µg
Conjugate/TagAPC
Clone7E9
ApplicationsFlow Cytometry (FC)
IsotypeIgG2a, Kappa
ReactivityMouse
Antibody TypePrimary
NCBI Full Gene Namecomplement receptor 2, complement component (3b/4b) receptor 1-like
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenCD35/CFA
ClonalityMonoclonal
TargetCD21/CD35

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Ultra‐high static magnetic fields cause immunosuppression through disrupting B‐cell peripheral differentiation and negatively regulating BCR signalingMedComm (2020)October 1, 2023Heng Gu, Yufan Fu, Biao Yu, Li Luo, Danqing Kang, Miaomiao Xie, Yukai Jing, Qiuyue Chen, Xin Zhang, Juan Lai, et al.whole operation was kept on ice and out of light. The antibodies used are as follows: APC‐anti‐CD21 (123412; BioLegend), BV421‐anti‐IgM (406518; BioLegend), BV510‐anti‐B220 (103234; BioLegend), FITC‐anti‐CD19

(2) GSDMA3 deficiency reprograms cellular metabolism and modulates BCR signaling in murine B cellsiScienceAugust 18, 2023Guan F, Luo X, Liu J, Huang Y, Liu Q, Chang J, Fang G, Kang D, Gu H, Luo L, et al.AB_2562185 Percp/Cy5.5 anti-mouse IgD,Biolegend,Cat # 405736; RRID: AB_2563346 APC anti-mouse CD21,Biolegend,Cat # 123412; RRID: AB_2085160 PE anti-mouse IgG1,BD,Cat # 550083; RRID: AB_393553 FITC anti-mouse IgA,BD,Cat #

(3) A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiationNature communicationsNovember 28, 2022Wu J, Yang K, Cai S, Zhang X, Hu L, Lin F, Wu SQ, Xiao C, Liu WH, Han J.file. Antibodies and reagents The following antibodies were from Biolegend: APC anti-mouse CD21 (123412, 1:200), APC anti-mouse CD117 (105812, 1:200), APC anti-mouse GL7 (144617, 1:200), APC-Cyanine7 anti-mouse CD69

(4) Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2Clinical and translational medicineJuly 1, 2022Zhu Y, Gu H, Yang L, Li N, Chen Q, Kang D, Lin S, Jing Y, Jiang P, Chen Q, et al.V (640906), ‐CD5 (100622), ‐CD19 (101506), ‐CD11b (101226). APC channel: anti‐CD43 (143208), ‐CD21 (123412). PE channel: anti‐BP‐1 (108307), ‐CD23 (101608). PE/Cy7 channel: anti‐CD24 (101822). PerCP/Cy5.5 channel:

(5) RNA exosome drives early B cell development via noncoding RNA processing mechanismsScience immunologyJune 3, 2022Laffleur B, Batista CR, Zhang W, Lim J, Yang B, Rossille D, Wu L, Estrella J, Rothschild G, Pefanis E, et al.557192), anti-IgM PE-Cy7 (clone R6–60.2, BD Biosciences 552867), anti-CD21 APC (clone 7E9, BioLegend 123411), anti-CD23 PE (clone B3B4, BD Biosciences 553139), anti-GL7 PE (clone GL7, eBioscience 12–5902-82),

(6) Protection of Quiescence and Longevity of IgG Memory B Cells by Mitochondrial AutophagyThe Journal of ImmunologyMarch 1, 2022Srikanth Kodali, Min Li, Marietta M. Budai, Min Chen, Jin Wang647-anti-IgG2a (407116), APC-anti-IgG2b (406712), APC-anti-IgG (405308), APC-anti-CD44 (103012), APC-anti-CD21 (123412), Alexa Fluor 488-anti-IgG1 (406626), Alexa Fluor 488-anti-GL-7 (144612) and FITC-anti-IgM (406506)

(7) Receptor Interacting Protein Kinase Pathways Regulate Innate B Cell Developmental Checkpoints But Not Effector Function in MiceFrontiers in ImmunologyDecember 9, 2021Raksha Parthasarathy, Thomas Hägglöf, Jason T. Hadley, Alexandra McLennan, Aiden Mattke, Elizabeth A. Dudley, Abigail Kumagai, Lily Q. Dong, Elizabeth A. Leadbetter

(8) The ubiquitin ligase HOIL-1L regulates immune responses by interacting with linear ubiquitin chainsiScienceNovember 19, 2021Gomez-Diaz C, Jonsson G, Schodl K, Deszcz L, Bestehorn A, Eislmayr K, Almagro J, Kavirayani A, Seida M, Fennell LM, et al.(BD Biosciences, 553164), TCRβ (BD Biosciences, 560729), CD23 (BioLegend, 101614), CD21 (BioLegend, 123411), Ly6G (BioLegend, 127618), Ly6C (BD Biosciences, 560595), TCRβ (BD Biosciences, 553170), B220 (BD Biosciences,

(9) The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in miceeLifeNovember 11, 2021Lehrke MJ, Shapiro MJ, Rajcula MJ, Kennedy MM, McCue SA, Medina KL, Shapiro VS.7E9)",BioLegend,Cat#:123416; RRID:AB_1595490,FACS (1:100) Antibody,"Anti-CD21/35 APC (Rat monoclonal, 7E9)",BioLegend,Cat#:123412; RRID:AB_2085160,FC (1:200) Antibody,"Anti-CD23 APC (Rat monoclonal, B3B4)",BioLegend,Cat#:101614;

(10) Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signalingThe EMBO journalDecember 15, 2020Fennell LM, Gomez Diaz C, Deszcz L, Kavirayani A, Hoffmann D, Yanagitani K, Schleiffer A, Mechtler K, Hagelkruys A, Penninger J, et al.

(11) Single-cell analysis reveals immune modulation and metabolic switch in tumor-draining lymph nodesOncoImmunologyOctober 29, 2020Yen-Liang Li, Chung-Hsing Chen, Jing-Yi Chen, You-Syuan Lai, Shao-Chun Wang, Shih-Sheng Jiang, Wen-Chun HungLakes, NJ, USA). Anti-CD8a (53–6.7, cat. 100734), anti-IgM (RMM-1, cat. 406531) and anti-CD21 (7E9, cat. 123411) were obtained from BioLegend (San Diego, CA, USA). For flow cytometry experiments, total eight axillary

(12) Checkpoint Receptor TIGIT Expressed on Tim-1⁺ B Cells Regulates Tissue InflammationCell reportsJuly 14, 2020Xiao S, Bod L, Pochet N, Kota SB, Hu D, Madi A, Kilpatrick J, Shi J, Ho A, Zhang H, et al.Clone 281–2",Biolegend,Cat# 142506; RRID: AB_10962911 "Anti-mouse CD21, Clone CR2/CR1",Biolegend,Cat# 123412; RRID: AB_2085160 "Anti-mouse CD23, Clone B3B4",Biolegend,Cat# 101606; RRID: AB_312831 "Anti-mouse

(13) Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B CellsCell metabolismApril 7, 2020Rosser EC, Piper CJM, Matei DE, Blair PA, Rendeiro AF, Orford M, Alber DG, Krausgruber T, Catalan D, Klein N, et al.RRID:AB_11218994 "CD19 BV785, Clone HIB19",Biolegend,Cat# 302240;RRID:AB_11218596 "CD21 APC, Clone 7G6",Biolegend,Cat# 123412; RRID:AB_2085160 "CD21 FITC, Clone 7G6",BD Biosciences,"Cat# 553818, RRID:AB_395070" "CD23 FITC, Clone

(14) Dysfunction of B Cell Leading to Failure of Immunoglobulin Response Is Ameliorated by Dietary Silk Peptide in 14-Month-Old C57BL/6 MiceFrontiers in nutritionJanuary 1, 2020Chei S, Oh HJ, Lee K, Jin H, Lee JY, Lee BY.this study: FITC anti-mouse CD3ε (#100306), FITC anti-mouse CD19 (#152404), APC anti-mouse CD21/CD35 (#123412), PerCP/Cyanine5.5 anti-mouse CD23 (#101618), and Brilliant Violet 421 anti-mouse IgD (#405725) (all

(15) Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune ResponsesFrontiers in immunologyJanuary 1, 2020Sarapulov AV, Petrov P, Hernández-Pérez S, Šuštar V, Kuokkanen E, Cords L, Samuel RVM, Vainio M, Fritzsche M, Carrasco YR, et al.anti-mouse/human CD45R/B220 [RA3-6B2],103224,BioLegend,Flow cytometry APC anti-mouse CD21/CD35 (CR2/CR1) [7E9],123412,BioLegend,Flow cytometry Alexa Fluor® 594 anti-mouse CD23 [B3B4],101628,BioLegend,Flow cytometry "PE anti-mouse CD93 (AA4.1, early B lineage

(16) An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph nodeeLifeDecember 6, 2019Chung Park, John H Kehrlmonoclonal)",BioLegend,304413,Dilution factor for FACS (1:500) Antibody,"anti-mouse CD21/35 (7E9), (Rat monoclonal)",BioLegend,"123421, 123411, 123409","BV421, PE, APC, Dilution factor for section (1:1000)" Antibody,"anti-mouse CD35 (8C12), (Rat

(17) Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B CellsCell reportsNovember 12, 2019Piper CJM, Rosser EC, Oleinika K, Nistala K, Krausgruber T, Rendeiro AF, Banos A, Drozdov I, Villa M, Thomson S, et al.BV785, Clone 6D5",Biolegend,Cat# 115543; RRID:AB_11218994 "CD21 APC, Clone CR2/CR1",Biolegend,Cat# 123412; RRID:AB_2085160 "CD23 FITC, Clone B3B4",Biolegend,Cat# 101606; RRID:AB_312831 "CD24 PE-Cy7, Clone

(18) Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiationThe Journal of allergy and clinical immunologyNovember 1, 2019Jing Y, Kang D, Liu L, Huang H, Chen A, Yang L, Jiang P, Li N, Miller H, Liu Z, et al.

(19) IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α⁺ Dendritic Cells Promotes Listeria monocytogenes InfectionImmunityJuly 16, 2019Liu D, Yin X, Olyha SJ, Nascimento MSL, Chen P, White T, Gowthaman U, Zhang T, Gertie JA, Zhang B, et al.(B3B4),BioLegend,"Cat# 101608, RRID:AB_312833" APC anti-mouse CD21/CD35 (CR2/CR1) Antibody (7E9),BioLegend,Cat# 123412 Pacific Blue™ anti-mouse F4/80 Antibody (CI:A3–1),BioLegend,"Cat# 123124, RRID:AB_893475" PerCP/Cy5.5

(20) Blimp-1 Contributes to the Development and Function of Regulatory B CellsFrontiers in immunologyJanuary 1, 2019Wang YH, Tsai DY, Ko YA, Yang TT, Lin IY, Hung KH, Lin KI.

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(1)

1/7/2019The Antibody Identifies CD21+ B Cells Very Well Robert YuanWe wanted to study B cells in the disease vs healthy state, so we used the antibody to identify CD21+ B cells in the spleen. The antibody is great to identify marginal zone B cells.

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APC anti-mouse CD21/CD35 (CR2/CR1) from BioLegend

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APC anti-mouse CD21/CD35 (CR2/CR1) from BioLegend

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