alpha Tubulin Antibody 62204 from Thermo Fisher Scientific

Product Specs
Itemalpha Tubulin Antibody
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog Number62204
Quantity40 µL
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Western Blot
Concentration0.5 mg/mL
Conjugate/TagUnconjugated
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityAmphibian, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Human, Mouse, Porcine, Rat
Targetalpha Tubulin
IsotypeIgG1 kappa
CloneDM1A
HostMouse
ClonalityMonoclonal
ImmunogenNative chick brain microtubules
NCBI Full Gene Nametubulin, alpha-like 3, tubulin, alpha 4A, tubulin, alpha 1A, tubulin, alpha 1C, tubulin, alpha 1B, tubulin alpha 4a, tubulin alpha-1C chain-like, tubulin alpha 3c, tubulin alpha 1b, tubulin alpha 3e, tubulin alpha 1c, tubulin beta 2B class IIb, tubulin, beta 2B class IIB, tubulin alpha 8A, tubulin, beta 2B class IIb, tubulin alpha 1a, tubulin alpha 8B, tubulin alpha 4A, tubulin alpha like 3
NCBI Gene AliasesTUBA8, 2410129E14Rik, bcm948, K-ALPHA-1, TUBA3, TUBA2, TUBA1, LIS3, TUBA6, RGD1565476, PMGYSA, TUBAT, TUBA3C, TUBA3E, TUBA3D, Tuba1, TUBA1C, Tuba2, Tuba1a, Tuba6, Tuba4, brdp, Tuba3e, bA408E5.3, TUBB2, bA506K6.1, M[a]6, M[a]4, TUBA4A, RGD1309427, TUBB2B, CDCBM7, TUBA1A, Tubb2, Tuba-1, ALS22, B-ALPHA-1, H2-ALPHA
Storage Temperature4° C
PurityProtein G
Antibody TypePrimary
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) SET7 methylates the deubiquitinase OTUB1 at Lys 122 to impair its binding to E2 enzyme UBC13 and relieve its suppressive role on ferroptosisJournal of Biological ChemistryApril 1, 2023Hongyan Deng, Shuke Jia, Jinhua Tang, Fangjing Rong, Chenxi Xu, Xiaoyun Chen, Zixuan Wang, Chunchun Zhu, Xueyi Sun, Qian Liao, et al.from Covance. Anti-Myc (#SC-40) antibody was purchased from Santa Cruz Biotechnology. Anti-α-tubulin (#62204) and Alexa Fluor 488 goat anti-rabbit IgG (#A11008) were purchased from Thermo Fisher Scientific. Erastin

(2) Alternative polyadenylation transcriptome-wide association study identifies APA-linked susceptibility genes in brain disordersNature communicationsFebruary 3, 2023Cui Y, Arnold FJ, Peng F, Wang D, Li JS, Michels S, Wagner EJ, La Spada AR, Li W.22309-1-AP, 1:3000), ATXN3 (Millipore-Sigma, MAB5360, clone 1H9, 1:2000), and α-tubulin (ThermoFisher, 62204, 1:3000). Cycloheximide chase experiment HEK293T cells were transfected with scramble shRNA or ATXN3

(3) Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cellsThe Journal of biological chemistryFebruary 1, 2023Hsu J, Fatuzzo N, Weng N, Michno W, Dong W, Kienle M, Dai Y, Pasca A, Abu-Remaileh M, Rasgon N, et al.(Biolegend #637312), CPT2 (Invitrogen #MA532314), GAPDH (Biolegend #649201), alpha Tubulin (Invitrogen #62204), HRP Donkey anti-rabbit IgG (minimal x-reactivity) (Biolegend #406401), and HRP Goat anti-mouse IgG (minimal x-reactivity

(4) Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genesNature cell biologyFebruary 1, 2023Sparbier CE, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan KL, Bell CC, Gilan O, Chan YC, Popp S, et al.anti-Menin (Abcam, Cat# ab2605; RRID: AB_303203), Monoclonal mouse alpha Tubulin (Invitrogen, Cat# 62204; clone DM1A; RRID: AB_1965960), Polyclonal rabbit anti-LEDGF/p75 (Bethyl, Cat# A300-847; RRID: AB_609466),

(5) Atypical TDP-43 protein expression in an ALS pedigree carrying a p.Y374X truncation mutation in TARDBPBrain pathology (Zurich, Switzerland)January 1, 2023Cooper-Knock J, Julian TH, Feneberg E, Highley JR, Sidra M, Turner MR, Talbot K, Ansorge O, Allen SP, Moll T, et al.to residues 203–209 of human TDP‐43 [17], and mouse anti‐tubulin antibody (Thermo Fisher Scientific; 62204, 1:5000) in 1% cow's milk TBST for 1 h at room temperature (RT). Membranes were washed and then blotted

(6) SETDB1 regulates microtubule dynamicsCell ProliferationDecember 1, 2022Rosari Hernandez‐Vicens, Jagreeti Singh, Nomi Pernicone, Tamar Listovsky, Gabi Gerlitzanti‐histone H3 (1:10,000, Millipore 05‐928), mouse anti‐α‐tubulin (1:5,000, Thermo Fisher Scientific 62204), mouse anti‐acetylated tubulin (1:1000, Santa Cruz Biotechnology sc‐23950), mouse anti‐β‐actin (1:5000,

(7) Methyltransferase SMYD3 impairs hypoxia tolerance by augmenting hypoxia signaling independent of its enzymatic activityThe Journal of biological chemistryDecember 1, 2022Wang Z, Chen X, Fan S, Zhu C, Deng H, Tang J, Sun X, Jia S, Liao Q, Xiao W, et al.and anti-GAPDH (#SC-477242) antibodies were purchased from Santa Cruz Biotechnology. Anti-α-tubulin (#62204), Alexa Fluor 488 goat anti-rabbit IgG (#A11008), Alexa Fluor 594 goat anti-mouse IgG (#A11005), CM-H2DCFDA

(8) Involvement of a Basic Helix-Loop-Helix Gene BHLHE40 in Specification of Chicken Retinal Pigment EpitheliumJournal of developmental biologyOctober 29, 2022Kinuhata T, Sato K, Bando T, Mito T, Miyaishi S, Nohno T, Ohuchi H.Roche; Merck, Darmstadt, Germany) or alpha tubulin (alpha Tubulin Monoclonal Antibody DM1A, 1:600, #62204; Thermo Fisher Scientific, Waltham, MA, USA). After washing four times for 10 min with Tris-buffered

(9) TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopyCell Rep MethodsOctober 24, 2022Steib E, Tetley R, Laine RF, Norris DP, Mao Y, Vermot J.tubulin,Thermofisher,Cat#32-2700; RRID:AB_2533073 "Alpha-tubulin, Mouse mono.",Life technologies,Cat#62204; RRID:AB_1965960 "Beta-tubulin, Mouse mono.",Life technologies,Cat#322600; RRID:AB_86547 "Myosin heavy

(10) Tight junction formation by a claudin mutant lacking the COOH-terminal PDZ domain-binding motifAnnals of the New York Academy of SciencesOctober 1, 2022Fujiwara S, Nguyen TP, Furuse K, Fukazawa Y, Otani T, Furuse M.polyclonal anti‐GFP (#A‐11122; Thermo Fisher Scientific), and mouse monoclonal anti‐α‐tubulin (Clone DM1A, #62204; Thermo Fisher Scientific). The following secondary antibodies were used: donkey anti‐mouse IgG Alexa

(11) Chitinase 3 like 1 contributes to the development of pulmonary vascular remodeling in pulmonary hypertensionJCI InsightSeptember 22, 2022Xiuna Sun, Erika Nakajima, Carmelissa Norbrun, Parand Sorkhdini, Alina Xiaoyu Yang, Dongqin Yang, Corey E. Ventetuolo, Julie Braza, Alexander Vang, Jason Aliotta, et al.antibodies for VE-cadherin (Abcam, ab119339), α-SMA (Abcam, ab5094), and tubulin (Thermo Fisher Scientific, 62204) were diluted with 5% BSA in TBST in a 1:500 ratio and added to respective membranes. After incubating

(12) CYP11A1‑derived vitamin D hydroxyderivatives as candidates for therapy of basal and squamous cell carcinomasInternational journal of oncologyAugust 1, 2022Slominski AT, Brożyna AA, Kim TK, Elsayed MM, Janjetovic Z, Qayyum S, Slominski RM, Oak ASW, Li C, Podgorska E, et al.temperature at 1:5,000 dilution). α-Tubulin: alpha Tubulin Antibody (Thermo Fisher Scientific, Inc.; cat. no. 62204) for primary antibody (1 h incubation at room temperature at 1:5,000 dilution) and goat anti-mouse IgG-HRP

(13) Overcoming Temozolomide Resistance in Glioblastoma via Enhanced NAD(+) Bioavailability and Inhibition of Poly-ADP-Ribose GlycohydrolaseCancersJuly 22, 2022Li J, Koczor CA, Saville KM, Hayat F, Beiser A, McClellan S, Migaud ME, Sobol RW."Cell Signaling Technology (Danvers, MA, USA)",3995S,1:1000 Tubulin,"Thermo Fisher Scientific (Waltham, MA, USA)",62204,1:1000 PAR,"Gift from Mathias Ziegler(University of Bergen, Bergen, Norway)",N/A,1:1000 Cleaved Caspase 3

(14) Colchicine-Binding Site Agent CH-2-77 as a Potent Tubulin Inhibitor Suppressing Triple-Negative Breast CancerMolecular cancer therapeuticsJuly 5, 2022Deng S, Krutilina RI, Hartman KL, Chen H, Parke DN, Wang R, Mahmud F, Ma D, Lukka PB, Meibohm B, et al.

(15) Peptide functionalized DNA hydrogel enhances neuroblastoma cell growth and differentiationNanoscaleJune 23, 2022Hivare P, Gangrade A, Swarup G, Bhavsar K, Singh A, Gupta R, Thareja P, Gupta S, Bhatia D.

(16) Factor quinolinone inhibitors disrupt spindles and multiple LSF (TFCP2)-protein interactions in mitosis, including with microtubule-associated proteinsPLOS ONEJune 15, 2022Sarah A. Yunes et al.formaldehyde. Primary antibodies included: mouse monoclonal anti-α-tubulin antibody (1:500, Thermo Fisher 62204), mouse monoclonal anti-γ-tubulin (1:1000, Abcam ab11316), rabbit polyclonal anti-α-tubulin (1:5000,

(17) PD-LI promotes rear retraction during persistent cell migration by altering integrin β4 dynamicsThe Journal of cell biologyMay 2, 2022Wang M, Xiong C, Mercurio AM.quarterly for mycoplasma. The following antibodies and reagents were used: anti–α-tubulin monoclonal DM1A (62204; Thermo Fisher Scientific), APC anti-human CD274 (B7-H1, PD-L1) antibody (329708; BioLegend), anti-mouse-PD-L1

(18) The Ethyl Acetate Fraction of Marine Sponge Stylissa carteri Induces Breast Cancer Cell Death via Upregulation of Mcl-1S: an In vitro StudyAsian Pacific journal of cancer prevention : APJCPMay 1, 2022Bashari MH, Fadhil M, Aulia Y, Sari AK, Putri T, Qomarilla N, Atmaja H, Sudji IR, Ariyanto EF, Indrati AR, et al.Gibco, USA. The primary antibodies of Mcl-1S (cat No. PA5-27597), α-tubulin primary antibody (cat No. 62204), and HRP secondary antibody (cat No. M32207) were bought from Invitrogen. Dimethyl sulfoxide (DMSO)

(19) DEK modulates both expression and alternative splicing of cancer‑related genesOncology ReportsApril 21, 2022Bin Liu, Yuanlin Sun, Yang Zhang, Yanpeng Xing, Jian Suoprimary antibodies, anti-DEK (1:1,000 dilution; ab166624, Abcam), anti-tubulin (1:2,000 dilution; #62204, Invitrogen; Thermo Fisher Scientific, Inc.) at 4°C overnight. Then, the membranes were incubated with

(20) MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylasesLeukemiaApril 1, 2022Lin AP, Qiu Z, Ethiraj P, Sasi B, Jaafar C, Rakheja D, Aguiar RCT.Cell Signaling Technology), β-actin (# A2228, Sigma-Aldrich), Lamin A (#A303–433A-M, Bethyl Laboratories), Tubulin (#62204 Invitrogen). PVDF membranes were stripped and re-probed with relevant antibodies for loading control,

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10/26/2022Great Loading Control Antibody Albert XuI used this tubulin antibody as a cytosolic marker for cellular fractionation experiments. This was a terrific antibody with no background and you get strong and clean signal.

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alpha Tubulin Antibody

alpha Tubulin Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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alpha Tubulin Antibody from Thermo Fisher Scientific

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