Anti-Tubulin antibody - Loading Control ab59680 from Abcam

Product Specs
ItemAnti-Tubulin antibody - Loading Control
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab59680
Antibody TypePrimary
Quantity50 UG
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
IsotypeIgG
HostRabbit
Conjugate/TagUnconjugated
Formatab59680 is affinity purified
Concentration0.5 mg/ml
Gene NameTUBA1B
ReactivityHuman
TargetTubulin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) RNA cytosine methyltransferase NSUN5 promotes protein synthesis and tumorigenic phenotypes in glioblastomaMolecular oncologySeptember 1, 2023Zhou J, Kong YS, Vincent KM, Dieters-Castator D, Bukhari AB, Glubrecht D, Liu RZ, Quilty D, Findlay SD, Huang X, et al.(Sigma‐Aldrich, Oakville, ON, Canada; Cat# A5441, RRID: AB_476744), and Tubulin (Abcam, Toronto, ON, Canada, ab59680). Immunofluorescence staining NSUN5 expression and intracellular localization of NSUN5 in GBM cells

(2) Sonic Hedgehog and WNT Signaling Regulate a Positive Feedback Loop Between Intestinal Epithelial and Stromal Cells to Promote Epithelial RegenerationCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Orzechowska-Licari EJ, Bialkowska AB, Yang VW.Centennial, CO; #NB600-600), SHH (1:1000; R&D Systems, Minneapolis, MN; #MAB464), tubulin (1:2000; Abcam; #ab59680), and β-actin (1:5000; Sigma-Aldrich; #A1978). The membranes were then washed in 1× TTBS, incubated

(3) Antiviral Effects of Curcumin on Adenovirus ReplicationMicroorganismsOctober 4, 2020Jennings MR, Parks RJ.tubulin (1/10,000 for 1 h incubation; CP06, Millipore), rabbit tubulin (1/10,000 for 1 h incubation; ab59680, Abcam, Cambridge, UK), anti-Adenovirus Type 5 (α-HAdV-5, 1/10,000 for 1 h incubation; ab6982, Abcam).

(4) Autophagy Inhibition Sensitizes Renal Tubular Epithelial Cell to G1 Arrest Induced by Transforming Growth Factor beta (TGF-β)Medical science monitor : international medical journal of experimental and clinical researchJune 7, 2020Yang C, Wu HL, Li ZH, Chen XC, Su HY, Guo XY, An N, Jing KP, Pan QJ, Liu HF.I (ab34710), cyclin-dependent kinase 4 (CDK4, ab137675), CDK6 (ab151247), cyclin D1 (ab16663), tubulin (Ab59680), b-actin (ab8227), and a-smooth muscle actin (a-SMA) were purchased from Abcam (Cambridge, MA, USA).

(5) Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal InjuryEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Huang YS, Wang X, Feng Z, Cui H, Zhu Z, Xia C, Han X, Liu WJ, Liu YN.and acetylated-p53 (ab61242; Abcam, Cambridge, MA, USA), GADPH (10494-1-AP, Proteintech), Tubulin (ab59680; Abcam, Cambridge, MA, USA), and horseradish peroxidase-conjugated secondary antibodies (SA0000I-I;

(6) Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesisNature CommunicationsDecember 1, 2019Natalie M. Niemi et al.Vogtle and/or Meisinger lab), anti-Por1p (Abcam #ab110326, dilution 1:1000), and anti-Tubulin (Abcam #ab59680; dilution 1:2000). Uncropped and unprocessed western blots from the main text can be found as Supplementary

(7) Increased UCP1 expression in the perirenal adipose tissue of patients with renal cell carcinomaOncology ReportsSeptember 10, 2019Xueqin Li, Gongcheng Wang, Juan Liu, Guoxian Dingagainst UCP1 (rabbit polyclonal, 1:1,000; Abcam; ab10983) or tubulin (rabbit polyclonal, 1:5,000; Abcam; ab59680) at 4°C for 12 h, and sequentially incubated with secondary antibodies (sheep anti-rabbit polyclonal

(8) RUNX3 Promotes the Tumorigenic Phenotype in KGN, a Human Granulosa Cell Tumor-Derived Cell LineInternational Journal of Molecular SciencesJuly 15, 2019Huachen Chen et al.Cell Signaling Technology (Danvers, MA, USA); antibodies against RUNX3 (ab40278), Cyclin D2 (ab207604), and tubulin (ab59680) were purchased from Abcam (Toronto, ON, Canada); antibodies against FLAG (F1804) and β-actin antibody

(9) Long non‑coding RNA plasmacytoma variant translocation 1 gene promotes the development of cervical cancer via the NF‑κB pathwayMolecular Medicine ReportsJuly 9, 2019Chang Wang, Hao Zou, Hongjuan Yang, Lei Wang, Huijun Chu, Jinwen Jiao, Yankui Wang, Aiping ChenkDa, dilution 1:10,000, cat. no. ab134175) and rabbit anti‑Tubulin antibody (55 kDa, dilution 1:5,000, cat. no. ab59680) served as an internal protein loading control (Abcam). Statistical analysis. All statistical analyses

(10) Tanshinone IIA Restores Dynamic Balance of Autophagosome/Autolysosome in Doxorubicin-Induced Cardiotoxicity via Targeting Beclin1/LAMP1CancersJune 28, 2019Wang X, Li C, Wang Q, Li W, Guo D, Zhang X, Shao M, Chen X, Ma L, Zhang Q, et al.anti-rabbit IgG H&L (ab16284; HRP; Abcam), anti-mouse IgG H&L (ab97250; HRP; Abcam), anti-tubulin (ab59680; Abcam), anti-histone H3 (ab1791; Abcam), and anti-TFEB (13372-1-AP; Proteintech, Rosemont, IL, USA).

(11) The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid (SAHA) Restores Cardiomyocyte Contractility in a Rat Model of Early DiabetesInternational Journal of Molecular SciencesApril 16, 2019Leonardo Bocchi et al.sc-376235), Acetylated-tubulin (1:1000, Sigma-Aldrich, St. Louis, MO, USA # T7451), Tubulin (1:2000, Abcam, Cambridge, UK, # ab59680), Ryanodine Receptor (C3-33) (1:1000, Abcam # ab2827), CACNA1c (1:500, Abcam # ab81095), NCX-1 (1:250,

(12) Long non-coding RNA-2271 promotes osteogenic differentiation in human bone marrow stem cellsOpen Life SciencesNovember 2, 2018Li-Cheng Xi, Hong-Yu Li, Dong Yinantibodies for either RUNX2 (1:500, ab76956, Abcam) or OSX (1:1,000, ab187158, Abcam), and tubulin (1:1,000, ab59680, Abcam) as a loading control. The membranes were then incubated with a corresponding horseradish peroxidase

(13) Mitochondrial complex I defect resulting from exercise-induced lower limb ischemia in patients with peripheral arterial diseaseJournal of Applied PhysiologySeptember 1, 2018I. Signolet et al.detection. Mouse anti-NDUFB6 (ab68331; Abcam) was used for CI detection, and anti-rabbit ␣-tubulin (ab59680; Abcam) and anti-mouse VDAC (ab61273; Abcam) were used as loading controls. Fluorescent secondary antibodies

(14) Differential fates of tissue macrophages in the cochlea during postnatal developmentHearing ResearchAugust 1, 2018Youyi Dong, Celia Zhang, Mitchell Frye, Weiping Yang, Dalian Ding, Ashu Sharma, Weiwei Guo, Bo Hua Huwhole-mount preparations were incubated overnight at 4°C with an anti-Tubulin antibody (Rabbit anti-Tubulin, ab59680, Abcam Inc., Cambridge, MA, USA) diluted in 1% Triton X-100 and 5% donkey serum in 0.1 M PBS (1:100).

(15) Sporamin suppresses growth of human esophageal squamous cell carcinoma cells by inhibition of NF-κB via an AKT-independent pathwayMolecular Medicine ReportsDecember 1, 2017Cui-Juan Qian, Yong-Xiao Qi, Xiao-Ying Chen, Ju-Ping Zeng, Jun Yaoantibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), and tubulin (catalog no. ab59680), histone (H)2A (catalog no. ab13923) and β‑actin (catalog no. ab8227) primary antibodies were purchased

(16) The Role of Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) in Dental Epithelium during Enamel Formation in MiceFrontiers in PhysiologyNovember 21, 2017Rozita Jalali, Johannes C. Lodder, Behrouz Zandieh-Doulabi, Dimitra Micha, James E. Melvin, Marcelo A. Catalan, Huibert D. Mansvelder, Pamela DenBesten, Antonius Bronckers(Abcam, #ab11370), mouse anti-β-actin antibody (Sigma, A2228) and rabbit anti-tubulin antibody (Abcam, ab59680). Secondary antibodies: IRDye 800CW conjugated goat anti-rabbit IgG (H+L) highly cross-adsorbed (LI-COR;

(17) Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localizationScientific reportsJuly 20, 2017Boeske A, Schwarten M, Ma P, Tusche M, Mötter J, Möller C, Neudecker P, Hoffmann S, Willbold D.Proteintech Group, Inc. (Chicago, IL, USA). The loading control antibody was the rabbit polyclonal anti-tubulin antibody (ab59680, Abcam, Cambridge, UK) whereas the horseradish peroxidase (HRP) conjugated secondary antibody from donkey

(18) Primate-specific oestrogen-responsive long non-coding RNAs regulate proliferation and viability of human breast cancer cellsOpen BiologyDecember 21, 2016Chin-Yo Lin et al.,Thermo Scientific 9101,phospho-p44/42 MAPK(Erk1/2),Cell Signaling 9102,p44/42 MAPK(Erk1/2),Cell Signaling ab59680,anti-tubulin 55kD,Abcam

(19) Notch and TGFß form a positive regulatory loop and regulate EMT in epithelial ovarian cancer cellsCellular SignallingMarch 31, 2016Jiesi Zhou, Saket Jain, Abul K. Azad, Xia Xu, Hai Chuan Yu, Zhihua Xu, Roseline Godbout, YangXin Fu

(20) PARP activation promotes nuclear AID accumulation in lymphoma cellsOncotargetMarch 15, 2016Sandra Tepper, Julia Jeschke, Katrin Böttcher, Angelika Schmidt, Kathrin Davari, Peter Müller, Elisabeth Kremmer, Peter Hemmerich, Ines Pfeil, Berit JungnickelE.K.), α-GFP (B-2, Santa Cruz Biotechnology Inc., USA), α-actin (A-2066, Sigma-Aldrich), α-tubulin (ab59680, Abcam), α-PARP (ab32071, Abcam), α-PChk1 (Ser345) (Cell Signalling Technologies #2341), α-PARylation

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Anti-Tubulin antibody - Loading Control from Abcam

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Anti-Tubulin antibody - Loading Control from Abcam

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