Anti-S6K1 (phospho T229) antibody ab59208 from Abcam

Product Specs
ItemAnti-S6K1 (phospho T229) antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab59208
Antibody TypePrimary
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
ClonalityPolyclonal
Quantity100 UG
IsotypeIgG
HostRabbit
Conjugate/TagUnconjugated
FormatAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site
Concentration1 mg/ml
ModificationsPhospho-T229
Gene NameRPS6KB1
ReactivityHuman
TargetS6K1

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Abcam

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United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) KLF5‑mediated expression of CARD11 promotes the progression of gastric cancerExperimental and Therapeutic MedicineJuly 17, 2023Qiusen Li, Sheng Li, Zongxian Li, Hongyan Xu, Wenxian Zhangab137676; Abcam), phosphorylated (p)-mTOR (1:1,000; cat. no. ab109268; Abcam), p-P70S6K (1:1,000; cat. no. ab59208; Abcam), mTOR (1:10,000; cat. no. ab134903; Abcam), P70S6K (1:5,000; cat. no. ab32529; Abcam) and GAPDH

(2) Raw Lacquer Extract from Toxicodendron vernicifluum in Combination with ONC201 Enhances the Inhibitory Effects on Colorectal Cancer Cell ActivityThe Turkish Journal of GastroenterologyMarch 14, 2023Zhezhu Jin, Department of Colorectal Surgery, Hangzhou Red Cross Hospital, Hangzhou Zhejiang, China, Yongjun Jin, Department of Colorectal Surgery, Hangzhou Red Cross Hospital, Hangzhou Zhejiang, China1:2500, Abcam), anti-p-mTOR (ab109268, 1:5000, Abcam), anti-S6K (ab32359, 1:2500, Abcam), anti-p-S6K (ab59208, 1:1000, Abcam), anti-β-actin (ab6276, 1:10000, Abcam) at 4°C overnight. The membranes were then washed

(3) Celastrol-regulated gut microbiota and bile acid metabolism alleviate hepatocellular carcinoma proliferation by regulating the interaction between FXR and RXRα in vivo and in vitroFrontiers in PharmacologyFebruary 15, 2023Dequan Zeng, Lipen Zhang, Qiang LuoCambridge, UK",Ab109268 Anti-S6K1,"Abcam, Cambridge, UK",Ab32529 Anti-p-S6K1,"Abcam, Cambridge, UK",Ab59208 Anti-p-Rb,"Abcam, Cambridge, UK",Ab184796 Anti-myc,"Abcam, Cambridge, UK",ab9106 Anti-flag,"Abcam,

(4) High Concentration of FBS Can Save mTOR Down-Regulation Caused by Mycoplasmas bovis InfectionVeterinary SciencesNovember 11, 2022Xiaochun Wu, Jinrui Ma, Shangdong Jia, Xudong Zhang, Xinlan Zhang, Zhen An, Yanquan Wei, Xiaoyong Xing, Fengqin Wen, Yuan Gao, et al.antibody (1:1000, PA5-36864, ThermoFisher, Rockford, IL, USA) and anti-phospho-T229 S6K antibody (1:2000, ab59208, Abcam, UK). Then, following three washes with TBST twice for 5 min each time, the membranes were incubated

(5) Upregulation of KIF18B facilitates malignant phenotype of esophageal squamous cell carcinoma by activating CDCA8 / mTORC1 pathwayJournal of Clinical Laboratory AnalysisOctober 1, 2022Jiangliu Xie, Bo Wang, Wenjie Luo, Chen Li, Xunchao Jia35 kDa, Abcam, UK, 1:1000), p70S6K (ab32529, 70 kDa, Abcam, UK, 1:5000), phosphorylated (p)‐p70S6K (ab59208, 60 kDa, Abcam, UK, 1:1000), p‐mTOR (ab109268, 289 kDa, Abcam, UK, 1:1000), mTOR (ab134903, 289 kDa,

(6) Impaired expression of BCAT1 relates to muscle atrophy of mouse model of sarcopeniaBMC musculoskeletal disordersMay 13, 2022Ouyang H, Gao X, Zhang J.was performed using the following antibodies: rabbit polyclonal anti-BCAT1 (13640–1-ap), anti-pS6K1 (ab59208; Abcam), rabbit monoclonal anti-p-mTOR (ab109268; Abcam), anti-mTOR (ab32028; Abcam), anti-S6K1 (ab32359;

(7) Everolimus (RAD001) combined with programmed death-1 (PD-1) blockade enhances radiosensitivity of cervical cancer and programmed death-ligand 1 (PD-L1) expression by blocking the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR)/S6 kinase 1 (S6K1) pathwayBioengineeredApril 29, 2022Lili Song, Shikai Liu, Sufen ZhaoAbcam), rabbit monoclonal anti-p-mTOR (1:1000, ab109268, Abcam), anti-p-S6 kinase 1 (S6K1; 1:1000, ab59208, Abcam), LC3B (1:1000, ab192890, Abcam), and p62 (1:1000, ab109012, Abcam) overnight at 4°C. Membranes

(8) GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathwayBioengineeredApril 28, 2022Haifeng Chen, Mingming Cheng, Pengcheng Gao, Xiangzhong Zhang, Ganggang Li, Liting Wang, Long Qin, Hongrui Li(ab52480), S6 (ab131526), p-S6 (ab32132), ribosomal protein S6 kinase (p70S6k, ab47504), and p-p70S6K (ab59208) purchased from Abcam (Cambridge, UK). After incubating primary antibodies, PVDF membranes were incubated

(9) Sepsis induces muscle atrophy by inhibiting proliferation and promoting apoptosis via PLK1-AKT signallingJournal of cellular and molecular medicineOctober 1, 2021Cao YY, Wang Z, Yu T, Zhang Y, Wang ZH, Lu ZM, Lu WH, Yu JB.,Santa Cruz Biotechnology,sc‐40,1:500 anti‐p‐mTOR,Abcam,ab109268,1:1000 anti‐mTOR,Abcam,ab134903,1:1000 anti‐p‐S6K,Abcam,ab59208,1:1000 anti‐ S6K,Abcam,ab186753,1:1000 anti‐p‐4EBP1,Abcam,ab259329,1:1000 anti‐4EBP1,Abcam,ab32024,1:1000 anti‐β‐actin,Proteintech,20536‐1‐AP,1:1000 The

(10) Rapamycin protects against aristolochic acid nephropathy in mice by potentiating mammalian target of rapamycin‑mediated autophagyMolecular medicine reportsJuly 1, 2021Lin F, Liu Y, Tang L, Xu X, Zhang X, Song Y, Chen B, Ren Y, Yang X.p-ribosomal S6 protein kinase (p-S6K1; phospho T252 for mouse and phospho T229 for human; cat. no. ab59208; 1:1,000; Abcam), S6K1 (cat. no. ab32359; 1:1,000; Abcam), p62 (cat. no. 13121; 1:1,000; Cell Signaling Technology

(11) EPHA2 Promotes the Invasion and Migration of Human Tongue Squamous Cell Carcinoma Cal-27 Cells by Enhancing AKT/mTOR Signaling PathwayBioMed Research InternationalMarch 24, 2021Fengqin Wang, Hanzhong Zhang, Zhigang ChengChina PAB35096 AB53699 AB40754 PAB39436 AB68182 PAB33726 AB8805 AB38449 AB32028 AB84400 AB131453 AB75767 AB32529 AB59208 PAB36264 SAB43711 1 : 1000 1 : 1000 1 : 2000 1 : 1000 1 : 1000 1 : 1000 1 : 1000 1 : 1000 1 : 2000 1 : 1000

(12) Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathwaysJournal of experimental & clinical cancer research : CRFebruary 12, 2021Liu S, Chen L, Chen H, Xu K, Peng X, Zhang M.anti-p-4E-BP1 (Cat. No. ab27792, Abcam, UK), anti-S6K1 (Cat. No. ab9366, Abcam, UK) anti-p-S6K1 (Cat. No. ab59208, Abcam, UK), HRP-conjugated secondary goat anti-rabbit antibody (Cat. No. SA00001–2, Proteintech, USA),

(13) Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathwayWorld journal of stem cellsJune 26, 2020Lei JH, Yan W, Luo CH, Guo YM, Zhang YY, Wang XH, Su XJ.(1:2000, ab226957, Abcam), ribosomal protein S6 kinase (S6K, 1:1000, ab186753, Abcam), p-S6K (1:1000, ab59208, Abcam), sex determining region Y-box 2 (SOX-2, 1:1000, PAB30154, Bioswamp), octamer-binding transcription

(14) Astragaloside IV protects retinal pigment epithelial cells from apoptosis by upregulating miR‑128 expression in diabetic ratsInternational Journal of Molecular MedicineApril 23, 2020Tao Wang, Zhongwei Zhang, Caiping Song, Lei Sun, Xinli Sui, Qun Qu, Jing LiuInc." AKT,"1:2,000",ab18785,Abcam p-AKT,"1:1,000",ab38449,Abcam p70s6k1,"1:1,500",ab32359,Abcam p-p70s6k1,1:500,ab59208,Abcam β-actin,"1:5,000",HC201,"TransGen Biotech Co., Ltd." Goat anti-rabbit,"1:3,000",ab6721,Abcam Goat

(15) AMPK directly activates mTORC2 to promote cell survival during acute energetic stressScience signalingJune 11, 2019Kazyken D, Magnuson B, Bodur C, Acosta-Jaquez HA, Zhang D, Tong X, Barnes TM, Steinl GK, Patterson NE, Altheim CH, et al.α-tubulin (#2144). mTOR P-Ser2481 was from Millipore (#09–343). S6K1 P-Thr229 antibody was from Abcam (#ab59208). The following custom polyclonal anti-peptide antibodies were generated by us with the aid of Covance,

(16) GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathwayInternational Journal of Molecular MedicineMarch 12, 2019Wei‑Si Lai, Yi‑Ling Ding(4E-BP1; 1:100, cat. no. ab2606), phosphoprotein 70 ribosomal protein S6 kinase (p70S6K; 1:100, cat. no. ab59208) and mTOR (1:100, cat, no. ab2732), and then washed three times in PBS for 5 min per wash. The above

(17) The 3-Phosphoinositide-Dependent Protein Kinase 1 Inhibits Rod Photoreceptor DevelopmentFrontiers in cell and developmental biologyJanuary 1, 2018Xing T, Hass DT, Zhang SS, Barnstable CJ.AC-15),Mouse,WB,1:2500,A1978,Sigma-Aldrich P70-S6K,Rabbit,WB,1:750,9202,Cell signaling p-P70-S6K (Thr252),Rabbit,WB,1:1000,ab59208,Abcam p-P70-S6K (Thr389),Rabbit,WB,1:1000,9205,Cell signaling PCNA (PC10),Mouse,IF,1:1000,2586,Cell signaling

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9/10/2019A Good Anti-S6K1 Antibody for Immunodetection in Human Cells Raj KumarExcellent antibody.

Anti-S6K1 (phospho T229) antibody from Abcam

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Anti-S6K1 (phospho T229) antibody from Abcam

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