Anti-SQSTM1 / p62 antibody - Autophagosome Marker ab56416 from Abcam

Product Specs
ItemAnti-SQSTM1 / p62 antibody - Autophagosome Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab56416
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity100 UG
ClonalityMonoclonal
IsotypeIgG2a
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Gene NameSQSTM1
ValidationKnockOut Validated
ReactivityHuman
TargetSQSTM1 / p62

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesityMolecular MetabolismJanuary 1, 2024Mengyang Tang, Yi Zhang, Rong Zhang, Yuemei Zhang, Jiangfei Zheng, Daixi Wang, Xinyu Wang, Jing Yan, Cheng Hu(T9026, Sigma-Aldrich, 1:1000), rabbit anti-GPSM1 (11483-1-AP, Proteintech, 1:1000), mouse anti-P62 (ab56416, Abcam, 1:1000), rabbit anti-LC3B (3868, CST, 1:1000), rabbit anti-PI3K (4757, CST, 1:1000), rabbit

(2) Berbamine suppresses intestinal SARS-CoV-2 infection via a BNIP3-dependent autophagy blockadeEmerging Microbes & InfectionsDecember 31, 2023Alexandra P. M. Cloherty, Anusca G. Rader, Kharishma S. Patel, Jimena Pérez-Vargas, Connor A. H. Thompson, Siobhan Ennis, Masahiro Niikura, Manon E. Wildenberg, Vanesa Muncan, Renée R. C. E. Schreurs, et al.serum albumin (BSA; both Sigma), followed by staining with either mouse anti-p62 (IgG2a, 5 μg/mL, Abcam ab56416) or mouse IgG2a isotype control (5 μg/mL, eBioscience 14-4724-85), and thereafter staining with goat anti-mouse IgG2a

(3) Nutlin-3a induces KRAS mutant/p53 wild type lung cancer specific methuosis-like cell death that is dependent on GFPT2J Exp Clin Cancer ResDecember 14, 2023Dasom Kim, Dongwha Min, Joohee Kim, Min Jung Kim, Yerim Seo, Byung Hwa Jung, Seung-Hae Kwon, Hyunju Ro, Seoee Lee, Jason K. Sa, et al.(Ser79) (#3661) were purchased from Cell Signaling Technology (Danvers, MA, USA); those against p62 (ab56416) and GFPT2 (ab190966) were purchased from Abcam (Cambridge, UK); those against Rac1 (610,650) were purchased

(4) Amyloidogenic light chains impair plasma cell survivalHaematologicaDecember 1, 2023Marjorie Pick, Eyal Lebel, Sharona Elgavish, Hadar Benyamini, Yuval Nevo, Rachel Hertz, Jacob Bar-Tana, Paola Rognoni, Giampaolo Merlini, Moshe E. Gattpolyclonal), κ – (1:20,000, ab134083 - polyclonal), c-Myc – (1:10,000, ab152146 - polyclonal), – p62 (1:1000, ab56416 - SQSTM1); Santa Cruz Biotechnology (TX, USA) – Cyclin E (1:200 of 200 mg/mL, SC-247-HE12), – GAPDH

(5) Autophagy drives the conversion of developmental neural stem cells to the adult quiescent stateNature CommunicationsNovember 24, 2023Isabel Calatayud-Baselga, Lucía Casares-Crespo, Carmina Franch-Ibáñez, José Guijarro-Nuez, Pascual Sanz, Helena Mira(Ser465/467) (D5B10) (1:500, Cell Signaling, #13820), Lamp2 (1:50, Biolegend, 108511), p62 (1:150, Abcam, ab56416), SOX2 (1:250, Gene Tex, GTX101507 and R&D, AF2018), GLAST biotin (1:50, Miltenyi, 130-119-161), Tax1bp1

(6) Activation of acetyl-CoA synthetase 2 mediates kidney injury in diabetic nephropathyJCI InsightOctober 23, 2023Jian Lu, Xue Qi Li, Pei Pei Chen, Jia Xiu Zhang, Liang Liu, Gui Hua Wang, Xiao Qi Liu, Ting Ting Jiang, Meng Ying Wang, Wen Tao Liu, et al.as an internal control. The following primary antibodies were used: anti-ACSS2 (ab133664), anti-p62 (ab56416), anti-S6K1 (ab32529), and anti–p-S6K1 (ab59208) purchased from Abcam; anti-nephrin (sc-376522) purchased

(7) Naringenin ameliorates collagen‐induced arthritis through activating AMPK‐mediated autophagy in macrophagesImmunity, Inflammation and DiseaseOctober 20, 2023Wei Zhang, Yuan Zhang, Jianguang Zhang, Chunbiao Deng, Chao Zhangmicrotubule‐associated protein 1A/1B‐light chain 3A/B (LC3A/B, #4108). Abcam provided the antibody against p62/sqstm1 (AB56416). 2.2 Animals We obtained male DBA/1 mice, free from pathogens, from Beijing Weitong Lihua Experimental

(8) Somatic variants of MAP3K3 are sufficient to cause cerebral and spinal cord cavernous malformationsBrainSeptember 1, 2023Jian Ren, Yazi Huang, Yeqing Ren, Tianqi Tu, Baoshan Qiu, Daosheng Ai, Zhanying Bi, Xue Bai, Fengzhi Li, Jun-Liszt Li, et al.CD31 (1:100; BD Biosciences, 550274), Collagen IV (1:100; Merk-millipore, AB756P), p62 (1:200; Abcam, ab56416), Laminin (1:400; Sigma, L9393), S6 Kinase (1:200, Cell Signaling Technologies, 34475, or cleaved Caspase-3

(9) Mitochondria are secreted in extracellular vesicles when lysosomal function is impairedNature communicationsAugust 18, 2023Liang W, Sagar S, Ravindran R, Najor RH, Quiles JM, Chi L, Diao RY, Woodall BP, Leon LJ, Zumaya E, et al.anti-Atg5 (12994S, 1:1000), anti-Atg7 (2631S, 1:1000) from Cell Signaling Technology; anti-SQSTM1/p62 (ab56416, 1:1000), anti-Tsg101 (ab83, 1:1000), anti-Calreticulin (ab2907, 1:1000) from Abcam; anti-Tim23 (11123-1-AP,

(10) Association of cyclooxygenase-2 expression with endoplasmic reticulum stress and autophagy in triple-negative breast cancerPLoS OneAugust 4, 2023Haechan Lee, SungWook Jung, Gyungyub Gong, Bora Lim, Hee Jin LeeMicrotubule-associated protein 1A/1B-light chain 3 beta (LC3b; 1:200, ab48394, Abcam), p62 (1:10000, ab56416, Abcam), and beclin1 (1:50, ab51031, Abcam) were used. The nuclear and cytoplasmic expression levels

(11) Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injuryRedox biologyAugust 1, 2023Xu Y, Geng Y, Wang H, Zhang H, Qi J, Li F, Hu X, Chen Y, Si H, Li Y, et al.ab104224, Cat# ab150077, Cat# ab183830, Cat# ab52636, Cat# ab207323, Cat# ab62344, Cat# ab180799, and Cat# ab56416, respectively). An anti-transcription factor EB (TFEB) antibody was purchased from Bethyl Laboratories

(12) HSPB8 frameshift mutant aggregates weaken chaperone-assisted selective autophagy in neuromyopathiesAutophagyAugust 1, 2023Tedesco B, Vendredy L, Adriaenssens E, Cozzi M, Asselbergh B, Crippa V, Cristofani R, Rusmini P, Ferrari V, Casarotto E, et al.04–263" SQSTM1/p62,Rabbit polyclonal,"1:2,000",,"Sigma-Aldrich, P0067" SQSTM1/p62,Mouse monoclonal,"1:2,000",1:500,"Abcam, ab56416" FLAG,Mouse monoclonal,"1:1,000",,"Sigma-Aldrich, F7425" PolyQ (3B5H10),Mouse monoclonal,"1:1,000",,"Sigma-Aldrich,

(13) Autophagy increase in Merosin-Deficient Congenital Muscular Dystrophy type 1AEuropean Journal of Translational MyologyJuly 28, 2023Mariangela Mastrapasqua, Roberta Rossi, Lucrezia De Cosmo, Annalisa Resta, Mariella Errede, Antonella Bizzoca, Stefania Zampatti, Nicoletta Resta, Emiliano Giardina, Maddalena Ruggieri, et al.ATG5 (1:100; Abcam, code ab78073), LC3b (1:300; Abcam, code ab48394), p62/SQSTM1 (1:50; Abcam, code ab56416), laminin-α2-β1-γ1 (1:200; Sigma-Aldrich, code L9393; Darmstadt, Germany), laminin-α2 (merosin; 1:20;

(14) RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegiaThe Journal of clinical investigationJuly 17, 2023Launay N, Ruiz M, Planas-Serra L, Verdura E, Rodríguez-Palmero A, Schlüter A, Goicoechea L, Guilera C, Casas J, Campelo F, et al.ab122370, 1:1,000), anti-RAB18 (Proteintech, catalog 11304-1-AP, 1:1,000), anti-p62 (Abcam, catalog ab56416, 1:1,000), anti-LC3B (Cell Signaling Technology, catalog 2775, 1:1,000), anti-VDAC1 (Abcam, catalog

(15) The UPR Maintains Proteostasis and the Viability and Function of Hippocampal Neurons in Adult MiceInternational Journal of Molecular SciencesJuly 16, 2023Pingting Liu, Md Razaul Karim, Ana Covelo, Yuan Yue, Michael K. Lee, Wensheng LinMillipore, RRID:AB_92930), ubiquitin (P4D1, 1:1000; Santa Cruz, sc8017), p62 (1:15,000; Abcam, catalog ab56416), CP13 (1:200; recognizes phosphorylated Ser202 of the tau protein, provided by Dr. Peter Davis at Alber

(16) The inhibition of FKBP5 protects β-cell survival under inflammation stress via AKT/FOXO1 signalingCell death discoveryJuly 14, 2023Liu N, Li R, Cao J, Song X, Ma W, Liu T, Wang L, Zou J, Zhang B, Liu Z, et al.MA, USA), PDX1(1:1000, CST-9679S, Cell signaling technology, Danvers, MA, USA), Sqstm1/p62 (1:1000, ab56416, Abcam, Cambridge, MA, USA), LC3I/II (1:1000, CST-12741S, Cell signaling technology, Danvers, MA, USA),

(17) Pectolinarigenin inhibits bladder urothelial carcinoma cell proliferation by regulating DNA damage/autophagy pathwaysCell death discoveryJuly 1, 2023Deng Z, Shen D, Yu M, Zhou F, Shan D, Fang Y, Jin W, Qian K, Li S, Wang G, et al.0.3% Triton X-100) for 1 h. Slips were incubated overnight at 4°C with primary antibodies p62 (Abcam, ab56416) or γ-H2AX (Abcam, ab81299), followed by incubation for 2 h with the secondary antibody (Invitrogen,

(18) Copper-dependent autophagic degradation of GPX4 drives ferroptosisAutophagyJuly 1, 2023Xue Q, Yan D, Chen X, Li X, Kang R, Klionsky DJ, Kroemer G, Chen X, Tang D, Liu J.,Cell Signaling Technology,9661 Ubiquitin,Santa Cruz Biotechnology,sc-8017 TAX1BP1,Proteintech,"14,424-1-AP" SQSTM1/p62,Abcam,ab56416 TFRC,Abcam,ab137844 CALCOCO2/NDP52,Proteintech,"66,401-1-IG" CCT2,Proteintech,"24,896-1-AP" OPTN,Proteintech,"60,293-1-IG" MAP1LC3A/B,Cell Signaling Technology

(19) Genetic spectrum and clinical features of adult leukoencephalopathies in a Chinese cohortAnnals of Clinical and Translational NeurologyJuly 1, 2023Minglei Liu, Yangyang Wang, Changhe Shi, Yanpeng Yuan, Lanjun Li, Xiaoyun Zhang, Yuming Xu, Jing Yangstained with hematoxylin and eosin, anti‐ubiquitin antibody (ab7780, Abcam, 1:400), or anti‐p62 antibody (ab56416, Abcam, 1:800). Photomicrographs were captured with a fluorescence microscope (Nikon, Eclipse Ni). Genetic

(20) Targeted degradation of ⍺-synuclein aggregates in Parkinson's disease using the AUTOTAC technologyMolecular neurodegenerationJune 24, 2023Lee J, Sung KW, Bae EJ, Yoon D, Kim D, Lee JS, Park DH, Park DY, Mun SR, Kwon SC, et al.experiments. Antibodies The followings are primary antibodies used: mouse monoclonal anti-SQSTM1/p62 (Abcam, ab56416; 1:20,000 for WB, 1:300 for ICC), rabbit polyclonal anti-LC3 (Sigma-Aldrich, L7543; 1:10,000 for WB,

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3/27/2018Anti-SQSTM1 / p62 Antibody Liyong Z.To test the apoptotic, necroptotic, and authopagic protein targets implicated in human normal fibroblasts treated with 3-MA, Western blotting was performed. Briefly, cells were seeded in 2% FBS medium... Read Review

Anti-SQSTM1 / p62 antibody - Autophagosome Marker from Abcam

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Anti-SQSTM1 / p62 antibody - Autophagosome Marker from Abcam

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