Fig 2: Overexpression of SNRPB reverses the effects of RUNDC3A-AS1 knockdown on cell proliferation and apoptosis. (a) RT-qPCR showed that SNRPB overexpression reversed the expression level of SNRPB caused by RUNDC3A-AS1 knockdown. (b) Cell viability was examined by the CCK-8 assay. (c, d) The EdU assay was conducted to detect cell proliferation. (e) Flow cytometry was used to test cell apoptosis. (f) The Western blot was employed to examine the protein level of apoptosis-related factors. *P < 0.05; ***P < 0.001.

Fig 3: SNRPB inhibits p53 expression: (a) western blotting of p53 protein level in PTC tissues and adjacent normal tissues, (b) IF staining for detecting expression of SNRPB and p53 in sh-NC- or sh-SNRPB#1-transfected PTC cells, (c and d) quantification of IF intensity of SNRPB and p53 and (e–g) Co-IP for determining the interaction between p53 and SNRPB in PTC cells. Student’s t-test was performed for statistical analysis. **p < 0.01.

Fig 4: SNRPB expression is positively correlated with cell cycle-related gene expression: (a–d) expression correlation between SNRPB and cell cycle-associated genes in THCA tissues shown by GEPIA database.

Fig 5: SNRPB knockdown induces G1 phase arrest in PTC cells: (a–c) RT-qPCR and western blotting for evaluating efficiency of SNRPB knockdown in PTC cells (TPC-1, IHH4), (d and e) flow cytometry for determining cell cycle distribution of PTC cells with treatment of mock, sh-NC or sh-SNRPB#1 and (f and g) western blotting for assessing levels of cell cycle-associated proteins in indicated PTC cells. ANOVA followed by Tukey’s post hoc analysis was performed for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001.