Fig 1: ARID1B and SUB1 co-regulated HOXA-AS2 expression. A, CO-IP for HOXA3 and ENO1, SFN, TRIM29; B, C, CHIP-PCR/QPCR for ARID1B and HOXA-AS2 promoter(upstream of the transcription start site 2000 bp), a 1-400 bp, b 400-800 bp, c 800-1200 bp, d 1200-1600 bp, e 1600-2000 bp, D, CO-IP for ARID1B and SUB1, E, F, RT-PCR and Western blot for detecting efficiency by down-regulation of ARID1B; G, Western blot for HOXA3 with down-regulation of ARID1B; H, J, RT-PCR showing SUB1 overexpression efficiency and accompanying expression of HOXA-AS2; I, RT-PCR for HOXA-AS2 with down-regulation of ARID1B; K, immunohistochemistry for ARID1B and HOXA3 in HB tissues and adjacent normal liver tissues, the scale bars were 200 µm. Data shown represent the average and SD of three independent experiments. *P < .05; **P < .01; ***P < .001