nephrin (B-12) Antibody sc-377246 from Santa Cruz Biotechnology, Inc.

Product Specs
Itemnephrin (B-12) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-377246
Quantity200 µg/ml
ApplicationsWB, IP, IF, ELISA
Conjugate/TagUnconjugated
Reactivitymouse, rat, human
ClonalityMonoclonal
CloneB-12
Hostmouse
Antibody TypePrimary Antibodies
NCBI Full Gene Namenephrosis 1, nephrin, NPHS1 adhesion molecule, nephrin
NCBI Gene AliasesCNF, NPHN, NephrinB, nephrin
Targetnephrin
FormatPurified
IsotypeIgG2a

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosisSignal transduction and targeted therapyMarch 13, 2023Du X, Ma X, Tan Y, Shao F, Li C, Zhao Y, Miao Y, Han L, Dang G, Song Y, et al.12217-1-AP) were purchased from Proteintech (Rosemont, IL, USA). Anti-CD31 (sc-18916), anti-nephrin (sc-377246) and anti-PDGFRβ (platelet-derived growth factor receptor beta, sc-374573) were purchased from Santa Cruz

(2) Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF-κB/NLRP3 AxisJournal of diabetes researchJanuary 1, 2023He J, Cui J, Shi Y, Wang T, Xin J, Li Y, Shan X, Zhu Z, Gao Y.pro-caspase-1 (ab179515), and IL-1β (ab9722) were purchased from Abcam (Cambridge, UK). Nephrin antibody (sc-377246) was obtained from Santa Cruz Biotechnology (Shanghai, China). MCC950 (B7946) and Z-YVAD-FMK (YVAD;

(3) Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivoFrontiers in Cell and Developmental BiologyNovember 18, 2022Weili Li, Jin Wang, Xiaojiao Yin, Huanhuan Shi, Benben Sun, Mengru Ji, Huichen Song, Jiachen Liu, Yihao Dou, Chenghong Xu, et al.ab207327), anti-SP-C (Santa Cruz Biotechnology, sc-518029), anti-nephrin (Santa Cruz Biotechnology, sc-377246) were used, and for sEV related organelles, anti-lamp2 (Santa Cruz Biotechnology, sc-18822), anti-Rab7

(4) cGAS-STING activation contributes to podocyte injury in diabetic kidney diseaseiScienceOctober 21, 2022Nan Zang, Chen Cui, Xinghong Guo, Jia Song, Huiqing Hu, Mengmeng Yang, Mingyue Xu, Lingshu Wang, Xinguo Hou, Qin He, et al.AB_2160592 Anti-Podocin,Bioss,Cat#bs-6597R; RRID: AB_11103353 Anti-Nephrin,Bioss,Cat#bs-4866R Anti-Nephrin,Santa Cruz,Cat#sc-377246 Anti-synaptopodin,Protech,Cat#21064-1-AP; RRID: AB_10733120 Anti-WT-1,Millipore,Cat#MAB4234; AB_570945 Anti-GM130,BD Biosciences

(5) Expression of Pannexin 1 in the Human Kidney during Embryonal, Early Fetal and Postnatal Development and Its Prognostic Significance in Diabetic NephropathyBiomedicinesApril 20, 2022Ivo Jeličić, Katarina Vukojević, Anita Racetin, Davor Čarić, Merica Glavina Durdov, Mirna Saraga-Babić, Natalija FilipovićNo.,Host,Dilution,Source Primary,Anti-Pannexin 1/PANX1,ABN242,Rabbit,1:300,"Merck KGaA, Darmstadt, Germany" Primary,Anti-Nephrin (B-12),sc-377246,Mouse,1:50,"Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA" Primary,Anti-Aquaporin 1/AQP1 (B-11),sc-25287,Mouse,1:50,"Santa Cruz Biotechnology Inc

(6) Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1(-/-) (yotari) MiceInternational journal of molecular sciencesFebruary 11, 2022Kelam N, Racetin A, Katsuyama Y, Vukojević K, Kostić S.Antibody,AF2105,Goat,1:300,"R&DSystems (Minneapolis, MN, USA)" Primary,Anti-nephrin Antibody (B-12),sc-377246,Mouse,1:50,"Santa Cruz Biotechnology, (Texas, TX, USA)" Secondary,"Anti-Goat IgG,Alexa Fluor® 594",705-295-003,Donkey,1:400,"Jackson Immuno

(7) ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyteRenal failureDecember 1, 2021Yuan S, Liang X, He W, Liang M, Jin J, He Q.one hour. Primary antibody LC3 antibody (Mitrin Biology, 14600-1-AP, 1:50) or nephrin (Santa Cruz, sc-377246, 1:100) was added to the cells and incubated at 4 °C overnight. After extensive washing with PBS, cells

(8) Tetrahydrocurcumin Ameliorates Kidney Injury and High Systolic Blood Pressure in High-Fat Diet-Induced Type 2 Diabetic MiceEndocrinology and MetabolismAugust 31, 2021Weerapon Sangartit, Kyung Bong Ha, Eun Soo Lee, Hong Min Kim, Upa Kukongviriyapan, Eun Young Lee, Choon Hee Chunginvestigated using TEM at 15,000× magnification. Western blot analysis Renal expression of nephrin (1:1,000, sc-377246), fibronectin (1:1,000, sc-8422), type IV collagen (1:1,000, sc-11360), ACE (1:1,000, sc-23909), NOX4

(9) Effects of Allicin on Pathophysiological Mechanisms during the Progression of Nephropathy Associated to DiabetesAntioxidants (Basel, Switzerland)November 15, 2020Arellano-Buendía AS, Castañeda-Lara LG, Loredo-Mendoza ML, García-Arroyo FE, Rojas-Morales P, Argüello-García R, Juárez-Rojas JG, Tapia E, Pedraza-Chaverri J, Sánchez-Lozada LG, et al.growth factor (VEGF) (GTX21316) were acquired from GeneTex (Irvine, CA, USA) and those for nephrin (sc-377246), insulin receptor substrate-1(IRS-1) (sc-515017), insulin receptor substrate-2 (IRS-2) (sc-390761), and

(10) Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTOR/NF-κB/NLRP3 AxisEvidence-Based Complementary and Alternative MedicineOctober 9, 2020Tao Wang, Yanbin Gao, Rongchuan Yue, Xiaolei Wang, Yimin Shi, Jiayi Xu, Bingjie Wu, Yimeng Li(ZSGB-BIO, Beijing, China) for 1 h, the sections were incubated overnight with rabbit antinephrin (1 : 50, sc-377246, Santa Cruz Biotech, CA, USA) antibody and rabbit anti-caspase-1 antibody (1 : 200, ab1872) at 4°C,

(11) Krüppel‑like factor 4 ameliorates diabetic kidney disease by activating autophagy via the mTOR pathwayMolecular medicine reportsOctober 1, 2019Gong J, Zhan H, Li Y, Zhang W, Jin J, He Q.primary antibodies at 4°C overnight: Anti-LC3 (Abcam; cat. no. ab192890; 1:500), anti-nephrin (cat. no. sc-377246; 1:50; Santa Cruz Biotechnology, Inc.), anti-filamentous (F-)actin (cat. no. ab130935; 1:100; Abcam),

(12) Calpain-10 drives podocyte apoptosis and renal injury in diabetic nephropathyDiabetes, Metabolic Syndrome and Obesity: Targets and TherapySeptember 11, 2019Tao Wang, Yanbin Gao, Xiaolei Wang, Yimin Shi, Jiayi Xu, Bingjie Wu, Jiaxin He, Yimeng Li1720 Email dfyynfm@163.com Materials and methods Reagents Mouse monoclonal anti-nephrin antibody (Cat#:sc-377246, LOT: G0213) was purchased from Santa Cruz Biotechnology, Inc. (CA, USA). Rabbit polyclonal anti-Calpain

(13) Protosappanin-A and oleanolic acid protect injured podocytes from apoptosis through inhibition of AKT-mTOR signalingCell Biology InternationalSeptember 8, 2019Jiaxin Zheng, Shoulin Zhang, Huijun Chen, Xiaojun Cai, Chunjian Zhang, Shuhua Li, Yabin Zhou, Jing Shang, Shunyu Liang, Fengzhen Yaoh at room temperature, and then incubated with specific primary antibodies against nephrin (1:500, Sc‐377246; Santa Cruz Biotechnology), podocin (1:1,500, Ab50339; Abcam), CD2AP (1:1,000, #2135; Cell Signaling Technology

(14) Exosome secreted from adipose-derived stem cells attenuates diabetic nephropathy by promoting autophagy flux and inhibiting apoptosis in podocyteStem cell research & therapyMarch 15, 2019Jin J, Shi Y, Gong J, Zhao L, Li Y, He Q, Huang H.CD44 (bs-4916R, 1:400, bioss), CD45 (bs-0522R, 1:300, bioss), CD90 (bs-0778R, 1:500, bioss), nephrin (sc-377246, 1:100, Santa Cruz), LC3 (12135-1-AP, 1:200, Proteintech) at 4 °C overnight. After incubation with secondary

(15) Icariin improves acute kidney injury and proteinuria in a rat model of pregnancy-induced hypertensionMolecular Medicine ReportsSeptember 19, 2017Wenyu Zhang, Wei Yuan, Ning Xu, Jinping Li, Wenxiu Chang(TBST), the membranes were incubated with the following primary antibodies, against nephrin (catalog no. sc-377246; 1:1,000; Santa Cruz Biotechnology, Inc.) and Ang II (catalog no. sc-20718; 1:500; Santa Cruz Biotechnology,

(16) Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathwayInternational Journal of Molecular MedicineMarch 1, 2017Guodong Huang et al.following antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA): nephrin (sc-377246), desmin (sc-65983), pro-caspase-3 (sc-7148), pro-caspase-9 (sc-56073), cleaved caspase-9 (sc-22182),

(17) Beneficial effects of Huaiqihuang on hyperglycemia-induced MPC5 podocyte dysfunction through the suppression of mitochondrial dysfunction and endoplasmic reticulum stressMolecular Medicine ReportsFebruary 1, 2017Ting-Xia Li, Jian-Hua Mao, Lei Huang, Hai-Dong Fu, Shuo-Hui Chen, Ai-Min Liu, Yu-Qin Liangmembranes were incubated with the following primary antibodies at 4˚C overnight: Anti‑nephrin (cat. no. sc‑377246; 1:1,000), anti‑GRP78 (cat. no. sc‑376768; 1:1,000), anti‑cleaved‑caspase‑3 (cat. no. sc‑271028; 1:1,000), anti‑β

(18) Activation of the IL-2 Receptor in Podocytes: A Potential Mechanism for Podocyte Injury in Idiopathic Nephrotic Syndrome?PloS oneJanuary 1, 2016Zea AH, Stewart T, Ascani J, Tate DJ, Finkel-Jimenez B, Wilk A, Reiss K, Smoyer WE, Aviles DH.Santa Cruz sc-8346 1:1,000 LC3 Novus Biologicals NB100-2220 1:5,000 Podocin Santa Cruz sc-21009 1:1,000 Nephrin Santa Cruz sc-377246 1:500 β-actin Sigma Aldrich A2228 1:10,000 doi:10.1371/journal.pone.0157907.t002 Island, NY).

(19) Mechanisms by which heme oxygenase rescue renal dysfunction in obesityRedox BiologySeptember 6, 2014Joseph Fomusi Ndisang, Shuchita TiwariCruz, CA, USA, c-kit (sc-365504), October 3/4 (sc-5279), pax-2 (sc-130387), WT1 (sc-192), nephrin (sc-377246), podocalyxin (sc-10506), podocin (sc-21009) and CD2AP (sc-9137)] and with beta-catenin ([E247] ab32572,

(20) Connexin 43 is Involved in Aldosterone-Induced Podocyte InjuryCellular Physiology and BiochemistrySeptember 2, 2014Min Yang, Bin Wang, Min Li, Bo Jiangserum albumin) for 1 h, the sections were incubated with primary antibodies against Cx43 and Nephrin (sc-377246, Santa Cruz) in PBS containing 3% BSA overnight at 4℃, Sections were then washed in PBS and incubated

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nephrin (B-12) Antibody from Santa Cruz Biotechnology, Inc.

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nephrin (B-12) Antibody from Santa Cruz Biotechnology, Inc.

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