PerCP anti-mouse CD4 100538 from BioLegend

Product Specs
ItemPerCP anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100538
NCBI Gene AliasesL3T4, Ly-4
ApplicationsFlow Cytometry (FC)
Conjugate/TagPERCP
CloneRM4-5
Quantity100 µg
IsotypeIgG2a, Kappa
ReactivityMouse
HostRat
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Active eosinophils regulate host defence and immune responses in colitisNatureMarch 2, 2023Alessandra Gurtner, Costanza Borrelli, Ignacio Gonzalez-Perez, Karsten Bach, Ilhan E. Acar, Nicolás G. Núñez, Daniel Crepaz, Kristina Handler, Vivian P. Vu, Atefeh Lafzi, et al.(M1/70, 101263), MHC-II AF700 (M5/114.15.2, 107622), Ly6G Percp-Cy5.5 (1A8, 127616), CD4 PerCP (RM4-5, 100538), TCRβ PE-Cy7 (H57-597, 109222), TCRβ PE-Cy7 (H57-597, 109228), CD80 BV605 (1:100, 16-10A1, 104729),

(2) Targeting TBK1 to overcome resistance to cancer immunotherapyNatureMarch 1, 2023Sun Y, Revach OY, Anderson S, Kessler EA, Wolfe CH, Jenney A, Mills CE, Robitschek EJ, Davis TGR, Kim S, et al.BioLegend), F4/80 (#157306, clone QA17A29, BioLegend), CD8α (#100749, clone 53-6.7, BioLegend), CD4 (100538, clone RM4-5, BioLegend), NK1.1 (#404-5941-82, clone PK136, Invitrogen), and TCRβ (#109220, clone H57-597,

(3) Preclinical Study of Plasmodium Immunotherapy Combined with Radiotherapy for Solid TumorsCellsNovember 14, 2022Zhu Tao, Wenting Ding, Zhipeng Cheng, Yinfang Feng, Zhongkui Kang, Runmin Qiu, Siting Zhao, Wen Hu, Fang Zhou, Donghai Wu, et al.surface antibodies (anti-CD45, Cat# 103147, Biolegend; anti-CD3, Cat# 100204, Biolegend; anti-CD4, Cat# 100538, Biolegend; anti-CD8a, Cat# 100752, Biolegend; anti-CD11b, Cat# 101261, Biolegend; anti-CD25, Cat# 102051,

(4) Mouse phospholipid phosphatase 6 regulates dendritic cell cholesterol, macropinocytosis, and allergen sensitizationiScienceOctober 21, 2022Thayse R. BrÃ¼ggemann, Troy Carlo, Nandini Krishnamoorthy, Melody G. Duvall, Raja-Elie E. Abdulnour, Julie Nijmeh, Hong Yong Peh, Harilaos Filippakis, Roxanne H. Croze, Byoungsook Goh, et al.AB_1134084 anti-mouse CD3,Biolegend,clone 17A2; cat#100219; RRID: AB_1732068 anti-mouse CD4,Biolegend,clone RM4-5; cat#100537; RRID: AB_893331 anti-mouse CD19,Biolegend,clone 1D3/CD19; cat#152403; RRID: AB_2629812 anti-mouse

(5) BCG hydrogel promotes CTSS-mediated antigen processing and presentation, thereby suppressing metastasis and prolonging survival in melanomaJournal for immunotherapy of cancerJune 1, 2022Kremenovic M, Chan AA, Feng B, Bäriswyl L, Robatel S, Gruber T, Tang L, Lee DJ, Schenk M.anti-CD3 (17A2, BioLegend, 100236), anti-CD8α (53–6.7, BioLegend, 100730), anti-CD4 (RM4-5, BioLegend, 100538), anti-CD11c (N418, BioLegend, 117336), anti-CD11b (M1/70, BioLegend, 101236), anti-F4/80 (BM8, BioLegend,

(6) Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancerThe Journal of biological chemistryApril 1, 2022Cha JH, Chan LC, Wang YN, Chu YY, Wang CH, Lee HH, Xia W, Shyu WC, Liu SP, Yao J, et al.(1:100; BioLegend, #100311), APC/Cy7-CD8a (1:100; BioLegend, #100713), and PreCP-CD4 (1:100; BioLegend, #100537). For further intracellular staining, cells were fixed, permeabilized, and stained with FITC-Granzyme

(7) Tim-3 Relieves Experimental Autoimmune Encephalomyelitis by Suppressing MHC-IIFrontiers in ImmunologyJanuary 13, 2022Lili Tang et al.2260623) for 4–6 h, washed with FACS once for staining with PerCP anti-mouse CD4 antibody (BioLegend, 100538), and then washed with FACS; 200 μl fixation/permer buffer (Invitrogen, 2220750) was added at 4°C overnight,

(8) EGFR-HIF1α signaling positively regulates the differentiation of IL-9 producing T helper cellsNature CommunicationsDecember 1, 2021Suyasha Roy, Zaigham Abbas Rizvi, Alexander J. Clarke, Felicity Macdonald, Abhaydeep Pandey, Dietmar Martin Werner Zaiss, Anna Kathrina Simon, Amit AwasthiCells were then stained with the cell surface antibodies- anti-mouse CD4 PerCP (RM4-5; BioLegend Cat # 100538; 1:200), anti-mouse CD62L APC (MEL-14; BioLegend Cat # 104412, 1:200), and anti-mouse CD25 PE (3C7,

(9) Elevated murine HB-EGF confers sensitivity to diphtheria toxin in EGFR-mutant lung adenocarcinomaDisease Models & MechanismsNovember 1, 2021Camila Robles-Oteiza, Deborah Ayeni, Stellar Levy, Robert J. Homer, Susan M. Kaech, Katerina Politifor surface staining: CD45 BV711 (1:400; #103147), CD3 APC-Cy7 (1:100; #100222), CD4 PerCP (1:200; #100538), CD8a BV605 (1:200; #100744), CD11b APC-Cy7 (1:200; #101226), CD11c PE-Cy7 (1:200; #117310), Ly6G PerCP

(10) p21 produces a bioactive secretome that places stressed cells under immunosurveillanceScience (New York, N.Y.)October 29, 2021Sturmlechner I, Zhang C, Sine CC, van Deursen EJ, Jeganathan KB, Hamada N, Grasic J, Friedman D, Stutchman JT, Can I, et al.FITC-conjugated anti-TCRβ (Tonbo Biosciences, #35–5961, 1:500), PerCP-conjugated rat anti-CD4 (BioLegend, #100538, 1:500), violetFluor 450-conjugated rat anti-CD8α (clone 2.43, Tonbo biosciences, #75–1886, 1:500),

(11) A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinomaCell Rep MethodsSeptember 27, 2021Fitzgerald B, Connolly KA, Cui C, Fagerberg E, Mariuzza DL, Hornick NI, Foster GG, William I, Cheung JF, Joshi NS.RRID:AB_2888931 CD44-BV711 (clone:IM7),BD Biosciences,Cat# 563971; RRID:AB_2738518 CD4-PERCP (RM4-5),BioLegend,Cat# 100537; RRID:AB_893331 CD8a-BUV395 (53-6.7),BD Biosciences,Cat# 565968; RRID:AB_2739421 PD1-BV711 (EH12.2H7),BioLegend,Cat#

(12) ST8Sia6 Promotes Tumor Growth in Mice by Inhibiting Immune ResponsesCancer Immunology ResearchAugust 1, 2021David J. Friedman, Sydney B. Crotts, Michael J. Shapiro, Matthew Rajcula, Shaylene McCue, Xin Liu, Khashayarsha Khazaie, Haidong Dong, Virginia Smith ShapiroSiglec-E-Fc (Cat. #551506, BioLegend), Streptavidin-PE-Cy7 (Cat. #405206, BioLegend), CD4-PerCP (Cat. #100538, BioLegend), CD8α-BV510 (Cat. #100752, BioLegend), CD25-BV785 (Cat. #102034, BioLegend), PD-1-FITC (Cat.

(13) SPNS2 enables T cell egress from lymph nodes during an immune responseCell reportsJuly 13, 2021Okuniewska M, Fang V, Baeyens A, Raghavan V, Lee JY, Littman DR, Schwab SR.(clone 145–2C11)",Biolegend,Cat# 100308; RRID: AB_312673 "CD4, PerCP (clone RMA 4–5)",Biolegend,Cat# 100537; RRID: AB_893331 "CD8, PE-Cy7 (clone 53–6.7)",Biolegend,Cat# 100721; RRID: AB_312760 "CD44, AF700

(14) Pro-resolving lipid mediator lipoxin A₄ attenuates neuro-inflammation by modulating T cell responses and modifies the spinal cord lipidomeCell reportsJune 1, 2021Derada Troletti C, Enzmann G, Chiurchiù V, Kamermans A, Tietz SM, Norris PC, Jahromi NH, Leuti A, van der Pol SMA, Schouten M, et al.

(15) Staphylococcus cohnii is a potentially biotherapeutic skin commensal alleviating skin inflammationCell reportsApril 27, 2021Ito Y, Sasaki T, Li Y, Tanoue T, Sugiura Y, Skelly AN, Suda W, Kawashima Y, Okahashi N, Watanabe E, et al.

(16) Critical role of WNK1 in MYC-dependent early mouse thymocyte developmenteLifeOctober 14, 2020Köchl R, Vanes L, Llorian Sopena M, Chakravarty P, Hartweger H, Fountain K, White A, Cowan J, Anderson G, Tybulewicz VL.100544; RRID:AB_11219790,FACS (1:200) Antibody,anti-CD4-PerCP (RM4-5) (Rat monoclonal),Biolegend,Cat# 100538; RRID:AB_893325,FACS (1:200) Antibody,anti-CD4-APC-CY7 (GK1.5) (Rat monoclonal),BD Pharmingen,Cat#

(17) IL-32γ potentiates tumor immunity in melanomaJCI insightSeptember 17, 2020Gruber T, Kremenovic M, Sadozai H, Rombini N, Baeriswyl L, Maibach F, Modlin RL, Gilliet M, von Werdt D, Hunger RE, et al.anti-CD3ε (145-2C11, BioLegend, 100306), anti-CD3 (17A2, BioLegend, 100236), anti-CD4 (RM4-5, BioLegend, 100538), anti-CD8α (53-6.7, BioLegend, 100730), anti-CD45.2 (104, BioLegend, 109830), anti-CD40 (3/23, BioLegend,

(18) CD81 Controls Beige Fat Progenitor Cell Growth and Energy Balance via FAK SignalingCellAugust 6, 2020Oguri Y, Shinoda K, Kim H, Alba DL, Bolus WR, Wang Q, Brown Z, Pradhan RN, Tajima K, Yoneshiro T, et al.CD3 Antibody Biolegend Cat# 100219; RRID:AB_1732068 PerCP anti-mouse CD4 Antibody Biolegend Cat# 100537; RRID:AB_893331 (Continued on next page) e1 Cell 182, 1–15.e1–e11, August 6, 2020 Please cite this

(19) IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125bCell death & diseaseJuly 30, 2020Yang R, Huang H, Cui S, Zhou Y, Zhang T, Zhou Y.anti-CD81 (sc-9158) antibodies were purchased from Santa Cruz Biotechnology. Anti-mouse CD4-PerCP (100538) and IL-17A-PE (506904) were purchased from Biolegend (San Diego, CA). Anti-Foxp3-PE (12-4771-82), Anti-CD4-APC

(20) Proteome analysis revealed the essential functions of protein phosphatase PP2A in the induction of Th9 cellsScientific reportsJuly 3, 2020Roy S, Goel R, Aggarwal S, Asthana S, Yadav AK, Awasthi A.Single cell suspension was prepared, then stained with the antibodies- anti-mouse CD4 PerCP (Biolegend; 100538), anti-mouse CD62L APC (Biolegend; 104412) and anti-mouse CD25 PE (Biolegend; 101904). Cells were sorted

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12/2/2015Excellent Antibody for Staining CD4+ T Cells Chantal KuhnOur laboratory is very interested in the role of regulatory T cells in autoimmunity. We mainly focus on regulatory CD4+FoxP3+ T cells and CD4+FoxP3-LAP+ T cells.

PerCP anti-mouse CD4 from BioLegend

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PerCP anti-mouse CD4 from BioLegend

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