Fig 1: AAV-DJ-82Q injection enhances mutant huntingtin (HTT) expression and induces neurodegeneration in the striatum. (A–E) Eight weeks after AAV-DJ-82Q-injection, the striata from sham, AAV-DJ-18Q, and AAV-DJ-82Q groups (n = 7 per group) were analyzed by Western blot to investigate change in protein expression of HTT (clone, 2Q75 and EM48), NeuN, DARPP32 and cleaved caspase-3 (A–C) and then quantified concerning the ratio of protein to total GAPDH for each sample (D). (A–C) show a representative Western blot. ANOVA test; #p < 0.05 and ##p < 0.01 vs. sham and AAV-DJ-18Q groups. (E–M) Striatal sections (n = 2 per brain) from each group (n = 7 per group) were accomplished by immunofluorescence stain using anti-HTT (clone, EM48, red) (E–G), NenN (green) (H–J), and cleaved caspase-3 (green) (K–M) antibodies. Scale bar denotes 100 µm.