Pacific Blue(TM) anti-mouse IFN-gamma 505818 from BioLegend

Product Specs
ItemPacific Blue(TM) anti-mouse IFN-gamma
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number505818
NCBI Gene AliasesIFN-g, If2f, Ifg
ReactivityMouse
HostRat
Quantity100 µg
CloneXMG1.2
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
IsotypeIgG1, Kappa
Conjugate/TagPacific Blue
NCBI Full Gene Nameinterferon gamma
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenE. coli-expressed, recombinant mouse IFN-γ
Antibody TypePrimary
ClonalityMonoclonal
TargetIFN-gamma

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) STING Is Required in Conventional Dendritic Cells for DNA Vaccine Induction of Type I T Helper Cell- Dependent Antibody ResponsesFrontiers in ImmunologyApril 22, 2022Justin Theophilus Ulrich-Lewis, Kevin E. Draves, Kelsey Roe, Megan A. O’Connor, Edward A. Clark, Deborah Heydenburg FullerThe following day, cells were stained for IL-2 (Biolegend cat. no. 503808), IFN-γ (Biolegend cat. no. 505818), FITC-conjugated anti-human/mouse Granzyme B (Biolegend cat. no. 515403) and TNF-α (Biolegend cat.no

(2) The ChAdOx1 vectored vaccine, AZD2816, induces strong immunogenicity against SARS-CoV-2 beta (B.1.351) and other variants of concern in preclinical studieseBioMedicineMarch 1, 2022Alexandra J Spencer et al.504126), IL10-PE (Clone JES5-16E3, 1 in 100, Cat No. 505008), IFNγ-e450 (Clone XMG1.2, 1 in 100, Cat No. 505818) and TNFα-A488 (Clone MP6-XT22, 1 in 100, Cat No. 506313) (unless stated all antibodies purchased from

(3) Histone Methyltransferase SETDB1 Promotes Immune Evasion in Colorectal Cancer via FOSB-Mediated Downregulation of MicroRNA-22 through BATF3/PD-L1 PathwayJournal of immunology researchJanuary 1, 2022Tian J, Wang W, Zhu J, Zhuang Y, Qi C, Cai Z, Yan W, Lu W, Shang A.Gladbach, Germany), fixed, permeabilized, and stained with Pacific blue-IFN-γ (BioLegend, San Diego, CA, #505817, rat, 1 : 50), followed by detection utilizing flow cytometer BD FACS Canto II (Immunocytometry Systems,

(4) Immunization with short peptide particles reveals a functional CD8⁺ T-cell neoepitope in a murine renal carcinoma modelJournal for immunotherapy of cancerDecember 1, 2021He X, Zhou S, Dolan M, Shi Y, Wang J, Quinn B, Jahagirdar D, Huang WC, Tsuji M, Pili R, et al.

(5) Separable Microneedle Patch to Protect and Deliver DNA Nanovaccines Against COVID-19ACS NanoSeptember 28, 2021Yue Yin et al.(Biolegend, 100203-1), APC-anti-CD8a (Biolegend, 100712), Pacific blue-anti-IFN-γ antibody (Biolegend, 505817), and PE-Cy7-IL-2 antibody (Biolegend, 503831) were purchased from Dakewe (Beijing, China). IgE, TNF-α,

(6) Splicing factor SRSF1 is indispensable for regulatory T cell homeostasis and functionCell reportsJuly 6, 2021Katsuyama T, Moulton VR.IL-17A,Biolegend,Cat #504109 Anti-mouse IL-17A,Biolegend,RRID: AB_493320 Anti-mouse IFN-γ,Biolegend,Cat #505818 Anti-mouse IFN-γ,Biolegend,RRID: AB_893526 Biotin anti-mouse CXCR5,Biolegend,Cat #145510 Biotin anti-mouse

(7) M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12BMolecular Therapy - OncolyticsMarch 26, 2021Yu-Shui Ma et al.Armenian hamster) and fixed and permeabilized with Pacific blue-IFN-γ (1:50, BioLegend, San Diego, CA, USA, #505817, rat). Subsequently, the cells were detected with a BD FACS Canto II flow cytometer (BD Immunocytometry

(8) Peripheral eosinophil counts predict efficacy of anti-CD19 CAR-T cell therapy against B-lineage non-Hodgkin lymphomaTheranosticsMarch 4, 2021Qingzhu Jia et al.were stained with anti-mouse CD8b PE (BioLegend #126608) and anti-mouse INF-γ Pacific Blue (BioLegend #505818). Flow cytometry was conducted with a BD FACSCantoTM II analyzer (BD Biosciences, Franklin Lakes, NJ,

(9) GC-Derived EVs Enriched with MicroRNA-675-3p Contribute to the MAPK/PD-L1-Mediated Tumor Immune Escape by Targeting CXXC4Molecular Therapy - Nucleic AcidsDecember 4, 2020Ping Li, Xingdong Luo, Yue Xie, Pengfei Li, Fangyong Hu, Junfeng Chu, Xiaojun Chen, Wenbo Song, Ali Wang, Guangyu Tian, et al.staining buffer (BD Biosciences, San Jose, CA, USA). PE-CD3 and permeated Pacific blue-IFN-γ (BioLegend, #505817, 1:50 ratio) were added into T cells, which were detected by a BD FACSCanto II flow cytometer (BD Biosciences)

(10) SARS-CoV-2 RBD Neutralizing Antibody Induction is Enhanced by Particulate VaccinationAdvanced materials (Deerfield Beach, Fla.)December 1, 2020Huang WC, Zhou S, He X, Chiem K, Mabrouk MT, Nissly RH, Bird IM, Strauss M, Sambhara S, Ortega J, et al.

(11) Exosomal miRNA-16-5p Derived From M1 Macrophages Enhances T Cell-Dependent Immune Response by Regulating PD-L1 in Gastric CancerFrontiers in Cell and Developmental BiologyNovember 30, 2020Zhengtian Li, Bing Suo, Gang Long, Yue Gao, Jia Song, Mengzhe Zhang, Baiyu Feng, Ce Shang, Dawei Wangwere incubated with PerCP-CD3 (#100326, Armenian Hamster, 1:100 Biolegend) and Pacific blue-IFNγ (#505817, Rat, 1:50, Biolegend) that was pre-fixed and pre-permeabilized. Cell apoptosis was assessed using a

(12) Long non-coding RNA SNHG12 promotes immune escape of ovarian cancer cells through their crosstalk with M2 macrophagesAgingSeptember 14, 2020Qian M, Ling W, Ruan Z.cells were stained with PE-CD3 (130-117-139, 1:50, Miltenyi Biotec) and Pacific blue-IFNγ (BioLegend, #505817, Rat, 1:50). Samples were analyzed by flow cytometry (BD Immunocytometry System) and FlowJo software

(13) CXXC finger protein 4 inhibits the CDK18-ERK1/2 axis to suppress the immune escape of gastric cancer cells with involvement of ELK1/MIR100HG pathwayJournal of cellular and molecular medicineSeptember 1, 2020Li P, Ge D, Li P, Hu F, Chu J, Chen X, Song W, Wang A, Tian G, Gu X.with PerCP‐CD3 (#100326; Armenian Hamster; 1:100; BioLegend) and Pacific blue‐interferon‐γ (IFN‐γ; #505817, Rat; 1:50; BioLegend), and Pacific blue‐IFN‐γ was added after immobilization and permeabilization.

(14) Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and MalignancyImmunityMay 19, 2020Milner JJ, Toma C, He Z, Kurd NS, Nguyen QP, McDonald B, Quezada L, Widjaja CE, Witherden DA, Crowl JT, et al.,Thermo Fisher Scientific,"Cat# MHGB05, RRID:AB_10373420" Pacific Blue anti-IFNϒ (XMG1.2),BioLegend,"Cat# 505818, RRID:AB_893526" PE/Cy7 anti-IL-2 (JES6-5H4),Thermo Fisher Scientific,"Cat# 25-7021-82, RRID:AB_1235004" eFluor

(15) Long non-coding RNA HOTTIP enhances IL-6 expression to potentiate immune escape of ovarian cancer cells by upregulating the expression of PD-L1 in neutrophilsJournal of Experimental & Clinical Cancer ResearchDecember 1, 2019Anquan Shang et al.Germany) was used for the separation of T cells and pacific blue-labeled interferon (IFN)-γ antibody (#505817, 1:50; BioLegend, San Diego, CA, USA) was used for the measurement of IFN-γ content. The detection of

(16) IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasionThe Journal of clinical investigationJuly 15, 2019Chan LC, Li CW, Xia W, Hsu JM, Lee HH, Cha JH, Wang HL, Yang WH, Yen EY, Chang WC, et al.

(17) Goblet cell-produced retinoic acid suppresses CD86 expression and IL-12 production in bone marrow-derived cellsInternational immunologySeptember 25, 2018Xiao Y, de Paiva CS, Yu Z, de Souza RG, Li DQ, Pflugfelder SC.

(18) Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1Molecular cellAugust 16, 2018Cha JH, Yang WH, Xia W, Wei Y, Chan LC, Lim SO, Li CW, Kim T, Chang SS, Lee HH, et al.BioLegend) and permeabilization (421402, BioLegend), intracellular INFγ was stained using INFγ-Pacific Blue (505817, 1:20; BioLegend) antibody. Stained T cells were analyzed by BD FACSCanto II (BD Biosciences) cytometer.

(19) An M2 Rather than a T _H 2 Response Contributes to Better Protection against Latency Reactivation following Ocular Infection of Naive Mice with a Recombinant Herpes Simplex Virus 1 Expressing Murine Interleukin-4Journal of VirologyMay 15, 2018Dhong Hyun Lee, Homayon Ghiasiwith allophycocyanin (APC)–IL-4 (catalog no. 504105; Biolegend) and Paciﬁc Blue–IFN-␥ (catalog no. 505817; Biolegend) anti-mouse Abs at 4°C for 30 min. Stained cells were washed twice with wash buffer and ﬁxed

(20) STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasionNature communicationsMay 15, 2018Hsu JM, Xia W, Hsu YH, Chan LC, Yu WH, Cha JH, Chen CT, Liao HW, Kuo CW, Khoo KH, et al.(BioLegend, #100713, 1:100), then fixed, permeabilized, and stained with Pacific blue-IFNγ (BioLegend, #505817, 1:50). Stained samples were evaluated by BD FACS Canto II (BD Immunocytometry Systems) and analyzed

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(1)

9/26/2017Anti-Mouse IFN-gamma Antibody (Pacific Blue) Jeeho LeeThe aim was to study Th1/Th17 development. The specific goal was to examine Th1 cells by IFN-gamma expression. I selected this product because it was of high quality and is cost effective.

Pacific Blue(TM) anti-mouse IFN-gamma from BioLegend

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Pacific Blue(TM) anti-mouse IFN-gamma from BioLegend

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