Anti-Otoferlin antibody [13A9] ab53233 from Abcam

Product Specs
ItemAnti-Otoferlin antibody [13A9]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab53233
Antibody TypePrimary
IsotypeIgG1
ClonalityMonoclonal
Clone13A9
Quantity100 UG
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence
HostMouse, Nude mouse
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaNeuroscience
Gene Nameotof
ReactivityHuman, Mouse
TargetOtoferlin

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Hyperacusis in the Adult Fmr1-KO Mouse Model of Fragile X Syndrome: The Therapeutic Relevance of Cochlear Alterations and BKCa ChannelsInternational journal of molecular sciencesJuly 24, 2023Ferraguto C, Bouleau Y, Peineau T, Dulon D, Pietropaolo S.temperature. IHCs were labeled with a mouse monoclonal antibody (Anti-Otoferlin1/400, AbCam, cat # ab53233). Synaptic ribbons were labeled with a guinea pig polyclonal antibody (Anti-RIBEYE 1/200, SYSY, cat

(2) Peroxisome Deficiency in Cochlear Hair Cells Causes Hearing Loss by Deregulating BK ChannelsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2023Fu X, Wan P, Lu L, Wan Y, Liu Z, Hong G, Cao S, Bi X, Zhou J, Qiao R, et al.Biosciences, 1:200), KCNMA1 (APC‐107, Alomone Labs, 1:200), TOMM20 (ab56783, Abcam, 1:200), otoferlin (ab53233, Abcam, 1:200), Ctbp2 (612 044, BD Transduction Laboratories, 1:200), GluR2 (MAB397, Millipore, 1:200),

(3) Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoformNature communicationsMay 29, 2023Liu H, Liu H, Wang L, Song L, Jiang G, Lu Q, Yang T, Peng H, Cai R, Zhao X, et al.IgG2a (Merck-Millipore, Germany, no: MAB397, dilution:1:200), mouse anti-otoferlin (Abcam, USA, no: ab53233, dilution:1:200), rabbit anti-otoferlin (Synaptic Systems, Germany, no: 178003, dilution:1:200), mouse

(4) MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochleaLife science allianceFebruary 1, 2022Ikäheimo K, Herranen A, Iivanainen V, Lankinen T, Aarnisalo AA, Sivonen V, Patel KA, Demir K, Saarma M, Lindahl M, et al.(CtBP2; BD Biosciences Cat. no. 612044, RRID: AB_399431, 1:200), mouse monoclonal otoferlin (Cat. no. ab53233, RRID: AB_881807, used at 1:500; Abcam). Affinity purified rabbit polyclonal PTPRQ antibody was a kind

(5) Neuronal development in the cochlea of a nonhuman primate model, the common marmosetDevelopmental neurobiologyNovember 1, 2021Hosoya M, Fujioka M, Murayama AY, Ozawa H, Okano H, Ogawa K.Germany",1:200 anti‐SYP,mouse,IgG1,s5768,"Merck KGaA, Darmstadt, GERMANY",1:300 anti‐OTOF,mouse,IgG1,ab53233,"Abcam, Cambridge, UK",1:100 anti‐SYT2,mouse,IgG2a,znp1,"DSHB, Iowa City, IA, USA",1:100 anti‐SYT4,rabbit,IgG,18977,"IBL,

(6) Complete Elimination of Peripheral Auditory Input Before Onset of Hearing Causes Long-Lasting Impaired Social Memory in MiceFrontiers in NeuroscienceJuly 27, 2021Rui Guo, Yang Li, Jiao Liu, Shusheng Gong, Ke Liuin this study were rabbit anti-MAP2 (1:500, Abcam, ab32454) and mouse anti-otoferlin (1:100, Abcam, ab53233). Secondary antibodies used were goat anti-mouse Alexa Fluor 488 (1:500, Invitrogen, Waltham, MA, United

(7) Synaptic Release Potentiation at Aging Auditory Ribbon SynapsesFrontiers in aging neuroscienceJanuary 1, 2021Peineau T, Belleudy S, Pietropaolo S, Bouleau Y, Dulon D.BD Biosciences, cat # 612044). Otoferlin was labeled with a mouse monoclonal antibody (1/200 AbCam, cat # ab53233). Autophagy was detected by labeling LC3B protein with anti-Rabbit monoclonal antibody [1/200 LC3B(D11)

(8) AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cellsJournal of cell scienceJanuary 22, 2020Kroll J, Özçete ÖD, Jung S, Maritzen T, Milosevic I, Wichmann C, Moser T.protein ribeye, 1:200, BD Biosciences, cat. no. 612044), mouse antiotoferlin (1:300, Abcam, cat. no. ab53233), rabbit anti-Vglut3 (1:300, Synaptic Systems, cat. no. 135 203), rabbit anti-Homer1 (1:200, Synaptic Systems,

(9) Overloaded Adeno-Associated Virus as a Novel Gene Therapeutic Tool for Otoferlin-Related DeafnessFrontiers in molecular neuroscienceJanuary 1, 2020Rankovic V, Vogl C, Dörje NM, Bahader I, Duque-Afonso CJ, Thirumalai A, Weber T, Kusch K, Strenzke N, Moser T.following primary antibodies were applied in GSDB overnight at 4°C: mouse anti-otoferlin (Cat.-Nr. ab53233; Abcam) or rabbit anti-otoferlin (Vogl et al., 2015; Al-Moyed et al., 2019), chicken anti-calretinin

(10) Endophilin‐A regulates presynaptic Ca ²⁺ influx and synaptic vesicle recycling in auditory hair cellsThe EMBO JournalMarch 1, 2019Jana Kroll, Lina M Jaime Tobón, Christian Vogl, Jakob Neef, Ilona Kondratiuk, Melanie König, Nicola Strenzke, Carolin Wichmann, Ira Milosevic, Tobias Moserprotein RIBEYE, 1:200, BD Biosciences, Cat. No. 612044), mouse antiotoferlin (1:300, Abcam, Cat. No. ab53233), rabbit anti-vGlut3 (1:300, Synaptic Systems, Cat. No. 135 203), rabbit anti-CaV1.3 (1:30, Alomone Labs

(11) A dual-AAV approach restores fast exocytosis and partially rescues auditory function in deaf otoferlin knock-out miceEMBO molecular medicineJanuary 1, 2019Al-Moyed H, Cepeda AP, Jung S, Moser T, Kügler S, Reisinger E.Healthcare Life Sciences). Membranes were probed with primary antibodies mouse IgG1 anti‐otoferlin [13A9] (#ab53233, Abcam, 1:500) and rabbit anti‐GAPDH (#247002, Synaptic Systems, 1:1,000) followed by incubation with

(12) Establishment of a Gentamicin Cochlear Poisoning Model in Guinea Pigs and Cochlear Nerve Endings Recognition of Ultrasound SignalsMedical Science MonitorDecember 28, 2018Fusen Wang, Shusheng Gong, Yuee Zhou, Chengcheng Huang, Tiegang Li, Qian Li, Xinyu Ceng, Chaoyan Wangincluding rabbit anti-Prestin (1: 200, Santa Cruz, SC-30163) and mouse anti-Otoferlin (1: 200, Abcam, AB53233), and Alexa Fluor 568 conjugated goat anti-rabbit IgG secondary antibody (1: 1000, Molecular probe,

(13) Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editingDevelopmentJune 13, 2018He Zhang et al.135203, Synaptic System), antiparvalbumin (mouse, 1:1000, P3088, Sigma) and anti-otoferlin (mouse, 1:200, ab53233, Abcam). All secondary antibodies that were compatible with different combinations of primary antibodies

(14) Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopyThe EMBO journalJanuary 4, 2018Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, et al.PBS containing 0.5% Triton X‐100 and 2.5% BSA. The primary antibodies mouse anti‐otoferlin (Abcam #ab53233), diluted 1:350, and rabbit anti‐ribeye (Synaptic Systems #192003), diluted 1:1,500, were applied for

(15) The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearingEMBO reportsNovember 1, 2017Christian Vogl et al.612044), mouse monoclonal antiacetylated tubulin (Sigma-Aldrich, T7451), mouse anti-otoferlin (Abcam, ab53233), mouse anti-beta2 spectrin (BD Bioscience, 612562), mouse anti-pericentrin (Abcam, ab28144), rabbit

(16) Different Ca_V1.3 Channel Isoforms Control Distinct Components of the Synaptic Vesicle Cycle in Auditory Inner Hair CellsThe Journal of neuroscience : the official journal of the Society for NeuroscienceMarch 15, 2017Vincent PF, Bouleau Y, Charpentier G, Emptoz A, Safieddine S, Petit C, Dulon D.polyclonal; Synaptic Systems, catalog #135 203) and anti-otoferlin (mouse monoclonal; Abcam, catalog #ab53233) antibodies, respectively. Actin-F was also used to visualize hair cells (1:100, Phalloidin Fluoprobe

(17) Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca²⁺ Affinity of the Multi-C₂-Domain Protein OtoferlinFrontiers in synaptic neuroscienceJanuary 1, 2017Meese S, Cepeda AP, Gahlen F, Adams CM, Ficner R, Ricci AJ, Heller S, Reisinger E, Herget M.(1% BSA and 0.1% Triton X-100 in PBS) for 1 h at RT. Antibodies to otoferlin (mouse, 1:400; Abcam, ab53233), myosin VI (rabbit, 1:400; Sigma, M5187), parvalbumin (1:2000, raised against bullfrog parvalbumin-3,

(18) Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearingThe EMBO journalDecember 1, 2016Christian Vogl, Iliana Panou, Gulnara Yamanbaeva, Carolin Wichmann, Sara J Mangosing, Fabio Vilardi, Artur A Indzhykulian, Tina Pangršic, Rosamaria Santarelli, Montserrat Rodriguez-Ballesteros, Thomas Weber, Sangyong Jung, Elena Cardenas, Xudong Wu, Sonja M Wojcik, Kelvin Y Kwan, Ignacio Del Castillo, Blanche Schwappach, Nicola Strenzke, David P Corey, Shuh-Yow Lin, Tobias Moser

(19) Hair cells use active zones with different voltage dependence of Ca2+ influx to decompose sounds into complementary neural codesProceedings of the National Academy of Sciences of the United States of AmericaAugust 9, 2016Ohn TL, Rutherford MA, Jing Z, Jung S, Duque-Afonso CJ, Hoch G, Picher MM, Scharinger A, Strenzke N, Moser T.

(20) Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing LossThe Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 6, 2016Carrott L, Bowl MR, Aguilar C, Johnson SL, Chessum L, West M, Morse S, Dorning J, Smart E, Hardisty-Hughes R, et al.Primary antibodies: goat anti-Np65 (1:60, R&D Systems, AF5360), mouse antiOtoferlin (1:100, Abcam, ab53233), rabbit anti-NF-200 (1:500, SigmaAldrich, N4142), mouse anti-NF-200 (1:150, Sigma-Aldrich, N0142), rabbit

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Anti-Otoferlin antibody [13A9] from Abcam

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Anti-Otoferlin antibody [13A9] from Abcam

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