Fig 1: Immunohistochemical analysis of ERG, PTEN, CRISP3 and SPINK1. Original magnification, ×200. (A) ERG expression is based on nuclear staining and classified as negative, weak, moderate and strong. (B) PTEN expression shows cytoplasmic staining classified as a 3-tiered system, and a mixed pattern is also noted. (C) CRISP3 expression is evaluated as cytoplasmic staining categorized as negative, weak, moderate and strong. (D) SPINK1 expression is assessed as negative and positive cytoplasmic staining. ERG, transcriptional regulator ERG; PTEN, phosphatase and tensin homolog; CRISP3, cysteine-rich secretory protein 3; SPINK1, serine protease inhibitor Kazal type I.