Anti-mH2A1 antibody ab37264 from Abcam

Product Specs
ItemAnti-mH2A1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab37264
Antibody TypePrimary
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Quantity100 UG
ClonalityPolyclonal
IsotypeIgG
Conjugate/TagUnconjugated
Concentration0.4 mg/ml
Research AreaEpigenetics
Gene NameH2AFY
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetmH2A1

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Distinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functionseLifeMarch 8, 2022Giorgio Oliviero, Sergey Kovalchuk, Adelina Rogowska-Wrzesinska, Veit SchwÃ¤mmle, Ole N Jensenpolyclonal),Abcam,Cat# ab1791;RRID:AB_302613,"WB (1:20,000)" Antibody,Anti-macroH2A.1 (rabbit polyclonal),Abcam,Cat# ab37264;RRID:AB_883064,WB (1:1000) Antibody,Anti-macroH2A2.2 (rabbit polyclonal),Abcam,Cat# ab4173;RRID:AB_2115110,WB

(2) A balanced Oct4 interactome is crucial for maintaining pluripotencyScience advancesFebruary 18, 2022Han D, Wu G, Chen R, Drexler HCA, MacCarthy CM, Kim KP, Adachi K, Gerovska D, Mavrommatis L, Bedzhov I, et al.(RING1B) (Cell Signaling Technology, #5694), and rabbit polyclonal anti-H2afy (macroH2A.1) (Abcam, ab37264). The membranes were then washed three times with PBST and incubated with the following horseradish

(3) Mutant U2AF1-induced alternative splicing of H2afy (macroH2A1) regulates B-lymphopoiesis in miceCell reportsAugust 31, 2021Kim SP, Srivatsan SN, Chavez M, Shirai CL, White BS, Ahmed T, Alberti MO, Shao J, Nunley R, White LS, et al.digested by shearing with sonication, and immunoprecipitation was performed with anti-H2AFY antibody (Cat# ab37264, Abcam) or normal Rabbit IgG (12–370, Millipore Sigma) and Protein A Dynabeads (Cat# 10001D, Thermo Fisher

(4) Poly(ADP-ribose) binding and macroH2A mediate recruitment and functions of KDM5A at DNA lesionsThe Journal of cell biologyJuly 5, 2021Kumbhar R, Sanchez A, Perren J, Gong F, Corujo D, Medina F, Devanathan SK, Xhemalce B, Matouschek A, Buschbeck M, et al.Sigma-Aldrich), rabbit anti-MBP (Ab9084; Abcam), mouse anti-Flag (F1804; Sigma-Aldrich), rabbit anti-macroH2A1 (Ab37264; Abcam), rabbit anti-macroH2A1.1 (12455S [D5F6N]; Cell Signaling Technology), mouse anti-macroH2A1.2

(5) The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formationNature communicationsJune 10, 2021Xu X, Ni K, He Y, Ren J, Sun C, Liu Y, Aladjem MI, Burkett S, Finney R, Ding X, et al.System (Bio-Rad). CHIP was carried out using the indicated antibodies (3 μg/sample): macroH2A1 (Abcam, ab37264), macroH2A2 (Abcam, ab102126), H3K4me3 (Abcam, ab8580), gamma H2AX (Abcam, ab20669,), BRCA1 (Bethyl,

(6) Trans- and cis-acting effects of Firre on epigenetic features of the inactive X chromosomeNature communicationsNovember 27, 2020Fang H, Bonora G, Lewandowski JP, Thakur J, Filippova GN, Henikoff S, Shendure J, Duan Z, Rinn JL, Deng X, et al.for H3K27me3 (Upstate/Millipore, #07–449), H2AK119ubi (Cell Signaling, #8240 S), macroH2A (Abcam, #ab37264), or NPM1 (Abcam, #ab10530) overnight at 4 °C in a humidified chamber cells/tissue sections were washed

(7) MacroH2A1 Immunoexpression in Breast CancerFrontiers in OncologyAugust 21, 2020Giuseppe Broggi et al.pretreatment. Then the slides were incubated overnight at 4 °C with rabbit polyclonal anti-macroH2A1 antibody (ab37264; Abcam, Cambridge, UK) diluted 1:200 in PBS (Sigma, Milan, Italy). Sections were counterstained with

(8) SirT7 auto-ADP-ribosylation regulates glucose starvation response through mH2A1Science advancesJuly 1, 2020Simonet NG, Thackray JK, Vazquez BN, Ianni A, Espinosa-Alcantud M, Morales-Sanfrutos J, Hurtado-Bagès S, Sabidó E, Buschbeck M, Tischfield J, et al.anti-mH2A1.1 (D5F6N, Cell Signaling Technology), anti-mH2A1.2 (#4827, Cell Signaling Technology), anti-mH2A1 (ab37264, Abcam), anti-mH2A.2 (gift from M.B.), anti-histone H2A (#12349, Cell Signaling Technology), anti-HA

(9) Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamicsNature communicationsMay 21, 2020Yu B, Ma J, Li J, Wang D, Wang Z, Wang S.Santa Cruz, sc-377000), anti-MMP3 (1:1000, Chemicon International, MAB1339), anti-macroH2A (1:1000, Abcam, ab37264) anti-phosphor-4EBP1 (37/46) (1:1000, Cell Signaling, 28555), anti-Axin1 (1:1000, Cell Signaling, 2087S),

(10) Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitinationNature communicationsMay 18, 2020Kelliher JL, West KL, Gong Q, Leung JWC.(Santa Cruz, sc-1616; 1:5000 for blotting), H2AZ (Cell Signaling, 2718; 1:1000 for blotting), MacroH2A (Abcam, ab37264: 1:1000 for blotting) and MacroH2A2 (Sigma Aldrich, HPA035865; 1:1000 for blotting). For western blotting,

(11) Increase of circulating IGFBP-4 following genotoxic stress and its implication for senescenceeLifeMarch 30, 2020Nicola Alessio, Tiziana Squillaro, Giovanni Di Bernardo, Giovanni Galano, Roberto De Rosa, Mariarosa A B Melone, Gianfranco Peluso, Umberto Galderisifrom Cell Signaling (MA, USA); Ki-67 (sc7846) from SantaCruz Biotechology (CA, USA); while MacroH2A1 (ab37264), H1.2 (ab4086), and ATM (ab36810) were obtained from ABCAM (UK). All of the antibodies were used according

(12) Dynamic Incorporation of Histone H3 Variants into Chromatin Is Essential for Acquisition of Aggressive Traits and Metastatic ColonizationCancer cellOctober 14, 2019Gomes AP, Ilter D, Low V, Rosenzweig A, Shen ZJ, Schild T, Rivas MA, Er EE, McNally DR, Mutvei AP, et al.(61475, Active Motif), histone H4 (ab10158, Abcam), histone H2A (ab18255, Abcam) and histone macro H2A.1 (ab37264, Abcam). Blots for total histone H3 were performed in the same membrane as histone H3.1/H3.2 blots after

(13) Immunoexpression of Macroh2a in Uveal MelanomaApplied SciencesAugust 8, 2019Salvatorelli, Puzzo, Bartoloni, Palmucci, Longo, Russo, Reibaldi, Vinciguerra, Volti, CaltabianoThen the slides were incubated overnight at 4 ◦ C with rabbit polyclonal anti-macroH2A.1 antibody (ab37264; Abcam, Cambridge, UK) diluted 1:200 in PBS (Sigma, Milan, Italy). Sections were counterstained with

(14) ZFR coordinates crosstalk between RNA decay and transcription in innate immunityNature CommunicationsDecember 1, 2018Nazmul Haque, Ryota Ouda, Chao Chen, Keiko Ozato, J. Robert Hoggblotted with primary antibodies against ZFR (custom mouse monoclonal, see below; 1:500), mH2A (Abcam, ab37264, 1:1000), IRF3 (Cell Signaling, 4302, 1:1000), phospho-IRF3 (Cell Signaling, 11904, 1:2000). For loading

(15) PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loadingeLifeJuly 9, 2018Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, Busino L, Rothenberg E, Pagano M.mCherry (1:1000, LifeSpan BioSciences cat. No. LS-C204826), macroH2A.1 (2 μL (ChIP), Abcam cat No. ab37264, RRID:AB_883064), RAD51 (1:500 (IF), GeneTex cat. No. GTX70230, RRID:AB_372856), RAD51 (1:1000 (WB),

(16) Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival GeneMolecular cellNovember 16, 2017Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R, Agrawal P, Chowdhury A, Gantz M, de Pablos-Aragoneses A, Morgenstern A, Wu P, et al.,Bethyl Laboratories,A301-985A H3K4me1,Abcam,ab8895 H3K4me3,Abcam,ab1012 H3K27ac,Abcam,ab177178 H3K27me3,EMD,07-449 MED1,Bethyl Laboratories,A300-793A macroH2A2,"Bernstein Laboratory, ISMMS",in house macroH2A1,Abcam,ab37264 CTCF,EMD,07-729 FOSL2,Santa Cruz,SC-604 TEAD4,Santa Cruz,SC-101184 HSP90,Santa Cruz,SC-13119 GAPDH,Santa Cruz

(17) The histone variant macroH2A confers functional robustness to the intestinal stem cell compartmentPLOS ONESeptember 21, 2017Ryan James Cedeno, Angela Nakauka-Ddamba, Maryam Yousefi, Stephanie Sterling, Nicolae Adrian Leu, Ning Li, John R. Pehrson, Christopher Joachim LengneriVision software. Specific primary antibodies and dilutions used were as follows: macroH2A1 (Abcam Ab37264, 1:200), macroH2A1.1 (CST #12455, 1:200), tdTomato (ClonTech 632392, 1:200), Ki67 (Abcam Ab15580, 1:200),

(18) Ki-67 Contributes to Normal Cell Cycle Progression and Inactive X Heterochromatin in p21 Checkpoint-Proficient Human CellsMolecular and Cellular BiologySeptember 1, 2017Xiaoming Sun, Aizhan Bizhanova, Timothy D. Matheson, Jun Yu, Lihua Julie Zhu, Paul D. Kaufmanantibody (MoBu-1) (ab8039; Abcam), mouse anti-p21 antibody (ab109520; Abcam), rabbit anti-mcroH2A.1 (ab37264; Abcam), mouse anti-Rb antibody (4H1) (9309; Cell Signaling), mouse anti-H4K20me1 (39727; Active Motif),

(19) ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repairGenes & developmentFebruary 1, 2017Leung JW, Makharashvili N, Agarwal P, Chiu LY, Pourpre R, Cammarata MB, Cannon JR, Sherker A, Durocher D, Brodbelt JS, et al.H2AX (Millipore, 07-627), γH2AX (Millipore, 05-636), H2AZ (Cell Signaling, 2718S), macroH2A (Abcam, AB37264), H2A.Bbd (Millipore, 06-1319), ZMYM3 (Abcam, AB106626), ATM (Santa Cruz Biotechnology, SC-135663),

(20) Epithelial-mesenchymal transition and FOXA genes during tobacco smoke carcinogen induced transformation of human bronchial epithelial cellsToxicology in VitroApril 19, 2016Audun Bersaas, Yke Jildouw Arnoldussen, Mari Sjøberg, Aage Haugen, Steen Mollerup

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Anti-mH2A1 antibody from Abcam

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Anti-mH2A1 antibody from Abcam

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